Recent advances in the use of DNA vaccines for the treatment of diseases of farmed animals

Hurk, Sylvia van Drunen Littel‐van den; Gerdts, Volker; Loehr, B. I.; Pontarollo, Reno; Rankin, Robert; Uwiera, Richard R. E.; Babiuk, Lorne A.

doi:10.1016/s0169-409x(00)00074-0

Cited by 41 publications

(19 citation statements)

References 59 publications

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“…Currently, DNA vaccines against BHV-1 induce an Ab response of low amplitude and short duration (1). One reason for the limited immune response induced by DNA vaccines might be the inability of plasmid-encoded Ag to be effectively acquired by APC.…”

Section: Discussionmentioning

confidence: 99%

Targeting with Bovine CD154 Enhances Humoral Immune Responses Induced by a DNA Vaccine in Sheep

Manoj

Griebel²,

Babiuk³

et al. 2003

The Journal of Immunology

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CD40-CD154 interactions play an important role in regulating humoral and cell-mediated immune responses. Recently, these interactions have been exploited for the development of therapeutic and preventive treatments. The objective of this study was to test the ability of bovine CD154 to target a plasmid-encoded Ag to CD40-expressing APCs. To achieve this, a plasmid coding for bovine CD154 fused to a truncated secreted form of bovine herpesvirus 1 glycoprotein D (tgD), pSLIAtgD-CD154, was constructed. The chimeric tgD-CD154 was expressed in vitro in COS-7 cells and reacted with both glycoprotein D- and CD154-specific Abs. Both tgD and tgD-CD154 were capable of binding to epithelial cells, whereas only tgD-CD154 bound to B cells. Furthermore, dual-labeling of ovine PBMCs revealed that tgD-CD154 was bound by primarily B cells. The functional integrity of the tgD-CD154 chimera was confirmed by the induction of both IL-4-dependent B cell proliferation and tgD-specific lymphoproliferative responses in vitro. Finally, sheep immunized with pSLIAtgD-CD154 developed a more rapid primary tgD-specific Ab response and a significantly stronger tgD-specific secondary response when compared with animals immunized with pSLIAtgD and control animals. Similarly, virus-neutralizing Ab titers were significantly higher after secondary immunization with pSLIAtgD-CD154. These results demonstrate that using CD154 to target plasmid-expressed Ag can significantly enhance immune responses induced by a DNA vaccine.

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Section: Discussionmentioning

confidence: 99%

Targeting with Bovine CD154 Enhances Humoral Immune Responses Induced by a DNA Vaccine in Sheep

Manoj

Griebel²,

Babiuk³

et al. 2003

The Journal of Immunology

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“…This elegant concept has been demonstrated successfully for a wide variety of vaccine antigens and has been effective in preventing both infectious diseases and cancer in mouse models [2]. Unfortunately, translating the success of DNA vaccines from mice to agriculturally important species [3] and humans [4] has not been very successful. This is illustrated by the fact that the only licensed DNA vaccine currently available is a vaccine for West Nile virus in horses [5].…”

Section: Introductionmentioning

confidence: 99%

A single HBsAg DNA vaccination in combination with electroporation elicits long-term antibody responses in sheep

Babiuk

Tsang

Hurk

et al. 2007

Bioelectrochemistry

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“…In addition, the ability of DNA vaccines to induce both humoral and cellular immune responses has been demonstrated in a number of human clinical trials and experimental models of infectious human diseases caused by viruses (4,25,39), intracellular bacteria (11,36), and parasites (20,32,38). The potential of DNA vaccination in domestic livestock and pet animals has also been explored (8,9,13,22), and several vaccines have now been licensed for veterinary use (2,3).…”

mentioning

confidence: 99%

A DNA Fusion Vaccine Induces Bactericidal Antibodies to a Peptide Epitope from the PorA Porin of Neisseria meningitidis

Zhu¹,

Williams

Rice³

et al. 2008

Infect Immun

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An experimental DNA plasmid vaccine was developed based on a well-characterized and protective peptide epitope derived from a bacterial porin protein. For this study, we used the P1.16b serosubtype epitope, located in variable region (VR)2 in loop 4 of the PorA outer membrane (OM) porin from Neisseria meningitidis serogroup B strain MC58. A plasmid that encoded the entire loop (pPorAloop4) was prepared, as well as a fusion plasmid that encoded the loop in tandem with the fragment C (FrC) immunostimulatory sequence from tetanus toxin (pPorAloop4-FrC). The constructs were used for intramuscular immunization without exogenous adjuvant. Murine antisera raised to the pPorAloop4-FrC DNA fusion plasmid reacted significantly with OMs in enzyme-linked immunosorbent assay and with whole bacteria by immunofluorescence, whereas antisera raised to the pPorAloop4 DNA plasmid and to control plasmid showed little or no reactivity. Significantly, only the pPorALoop4-FrC plasmid induced bactericidal antibodies, demonstrating that the intrinsic immunostimulatory sequence was essential for inducing a protective immune response. The antibodies raised to the P1.16b pPorALoop4-FrC plasmid were serosubtype specific, showing no significant immunofluorescence reactivity or bactericidal activity against other PorA variants. These data provide proof of principle for a DNA fusion plasmid strategy as a novel approach to preparing vaccines based on defined, protective epitopes.

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Recent advances in the use of DNA vaccines for the treatment of diseases of farmed animals

Cited by 41 publications

References 59 publications

Targeting with Bovine CD154 Enhances Humoral Immune Responses Induced by a DNA Vaccine in Sheep

Targeting with Bovine CD154 Enhances Humoral Immune Responses Induced by a DNA Vaccine in Sheep

A single HBsAg DNA vaccination in combination with electroporation elicits long-term antibody responses in sheep

A DNA Fusion Vaccine Induces Bactericidal Antibodies to a Peptide Epitope from the PorA Porin of Neisseria meningitidis

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