Recent Advances in the Study of Protein Imprinting

Zhou, Xin; Li, Wen‐You; He, Xiwen; Chen, Langxing; Zhang, Yukui

doi:10.1080/15422110701771425

Cited by 38 publications

(8 citation statements)

References 57 publications

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“…This dynamic range offers a LSPR sensitivity of 0.25 nm/nM and a detection limit of 13 nM or 0.32 μg/mL of NGAL (the wavelength precision of the optical detection system is ± 0.1 nm). This performance surpasses that afforded by the molecular imprinting of other traditional techniques such as quartz crystal microbalance and liquid chromatography, [40] and is very suitable for the detection of many proteins at clinical concentrations including NGAL biomarkers after kidney injury. [21] However, the challenge remains to approach the performance of natural antibodies in a comparable detection platform.…”

Section: Resultsmentioning

confidence: 99%

Hot Spot‐Localized Artificial Antibodies for Label‐Free Plasmonic Biosensing

Abbas

Tian

Morrissey

et al. 2012

Adv Funct Materials

101

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The development of biomolecular imprinting over the last decade has raised promising perspectives in replacing natural antibodies with artificial antibodies. A significant number of reports have been dedicated to imprinting of organic and inorganic nanostructures, but very few were performed on nanomaterials with a transduction function. Herein we describe a relatively fast and efficient plasmonic hot spot-localized surface imprinting of gold nanorods using reversible template immobilization and siloxane co-polymerization. The technique enables a fine control of the imprinting process at the nanometer scale and provides a nanobiosensor with high selectivity and reusability. Proof of concept is established by the detection of neutrophil gelatinase-associated lipocalin (NGAL), a biomarker for acute kidney injury, using localized surface plasmon resonance spectroscopy. The work represents a valuable step towards plasmonic nanobiosensors with synthetic antibodies for label-free and cost-efficient diagnostic assays. We expect that this novel class of surface imprinted plasmonic nanomaterials will open up new possibilities in advancing biomedical applications of plasmonic nanostructures.

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Section: Resultsmentioning

confidence: 99%

Hot Spot‐Localized Artificial Antibodies for Label‐Free Plasmonic Biosensing

Abbas

Tian

Morrissey

et al. 2012

Adv Funct Materials

101

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“…The number of reviews on the topic of molecular imprinting has continued to increase, which is a direct reflection of the rapidly expanding primary literature covering the area. The increasing incidence of molecular imprinting‐related material in reviews from other fields indicates that molecular imprinting science and technology has established itself within the broader scientific community…”

Section: Reviews and Monographsmentioning

confidence: 99%

Molecular imprinting science and technology: a survey of the literature for the years 2004-2011

2014

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SummaryHerein we present a survey of the literature covering the development of molecular imprinting science and technology over the years 2004 to 2011. In total, 3779 references to the original papers, reviews, edited volumes and monographs from this period are included, along with recently identified uncited materials from prior to 2004 which were omitted in the first instalment of this series covering the years 1930 to 2003. 1 In the presentation of the assembled references, a section presenting reviews and monographs covering the area is followed by sections describing fundamental aspects of molecular imprinting including the development of novel polymer formats. Thereafter, literature describing efforts to apply these polymeric materials to a range of application areas is presented. Current trends and areas of rapid development are discussed.2

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“…MI polymers (MIPs) have been used for specific recognition of different molecules, such as drugs and peptides. However, protein imprinting had a limited success due to their large molecular size [9]. Different studies used conventional methacrylate chemistries to prepare protein MIPs [10].…”

Section: Introductionmentioning

confidence: 99%

Soft-molecular imprinted electrospun scaffolds to mimic specific biological tissues

et al. 2018

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The fabrication of bioactive scaffolds able to mimic the in vivo cellular microenvironment is a challenge for regenerative medicine. The creation of sites for the selective binding of specific endogenous proteins represents an attractive strategy to fabricate scaffolds able to elicit specific cell response. Here, electrospinning (ESP) and soft-molecular imprinting (soft-MI) techniques were combined to fabricate a soft-molecular imprinted electrospun bioactive scaffold (SMIES) for tissue regeneration. Scaffolds functionalized using different proteins and growth factors (GFs) arranged onto the surface were designed, fabricated and validated with different polyesters, demonstrating the versatility of the developed approach. The scaffolds bound selectively each of the different proteins used, indicating that the soft-MI method allowed fabricating high affinity binding sites on ESP fibers compared to non-imprinted ones. The imprinting of ESP fibers with several GFs resulted in a significant effect on cell behavior. FGF-2 imprinted SMIES promoted cell proliferation and metabolic activity. BMP-2 and TGF-β3 imprinted SMIES promoted cellular differentiation. These scaffolds hold the potential to be used in a cell-free approach to steer endogenous tissue regeneration in several regenerative medicine applications.

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Recent Advances in the Study of Protein Imprinting

Cited by 38 publications

References 57 publications

Hot Spot‐Localized Artificial Antibodies for Label‐Free Plasmonic Biosensing

Hot Spot‐Localized Artificial Antibodies for Label‐Free Plasmonic Biosensing

Molecular imprinting science and technology: a survey of the literature for the years 2004-2011

Soft-molecular imprinted electrospun scaffolds to mimic specific biological tissues

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