Recent advances in the production of platform chemicals using metabolically engineered microorganisms

Kim, Ji Yeon; Ahn, Yeah-Ji; Lee, Jong An; Lee, Sang Yup

doi:10.1016/j.cogsc.2023.100777

Cited by 8 publications

(4 citation statements)

References 52 publications

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“… 165 In recent years, great progress has been made in the production of bio-based chemicals with the help of advanced tools and strategies for systems metabolic engineering. 166 …”

Section: Genetic Engineering For Microalgal Hydrogen Production Using...mentioning

confidence: 99%

Genetic engineering for biohydrogen production from microalgae

Zhang¹,

Xue²,

Wang³

et al. 2023

iScience

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“… 165 In recent years, great progress has been made in the production of bio-based chemicals with the help of advanced tools and strategies for systems metabolic engineering. 166 …”

Section: Genetic Engineering For Microalgal Hydrogen Production Using...mentioning

confidence: 99%

Genetic engineering for biohydrogen production from microalgae

Zhang¹,

Xue²,

Wang³

et al. 2023

iScience

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“…Nevertheless, there is mounting pressure to shift towards the production of bio-based polymers using monomers sourced from renewable resources [ 34 ]. This growing impetus has spurred considerable efforts towards the production of SA and BD through fermentation processes, leading to successful commercial production initiatives that have been announced and are currently expanding [ 35 , 36 , 37 ].…”

Section: Introductionmentioning

confidence: 99%

Ductile Copolyesters Prepared Using Succinic Acid, 1,4-Butanediol, and Bis(2-hydroxyethyl) Terephthalate with Minimizing Generation of Tetrahydrofuran

Park,

Seo,

Seo

et al. 2024

Polymers

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Poly(1,4-butylene succinate) (PBS) is a promising sustainable and biodegradable synthetic polyester. In this study, we synthesized PBS-based copolyesters by incorporating 5–20 mol% of –O2CC6H4CO2– and –OCH2CH2O– units through the polycondensation of succinic acid (SA) with 1,4-butanediol (BD) and bis(2-hydroxyethyl) terephthalate (BHET). Two different catalysts, H3PO4 and the conventional catalyst (nBuO)4Ti, were used comparatively in the synthesis process. The copolyesters produced using the former were treated with M(2-ethylhexanoate)2 (M = Mg, Zn, Mn) to connect the chains through ionic interactions between M2+ ions and either –CH2OP(O)(OH)O− or (–CH2O)2P(O)O− groups. By incorporating BHET units (i.e., –O2CC6H4CO2– and –OCH2CH2O–), the resulting copolyesters exhibited improved ductile properties with enhanced elongation at break, albeit with reduced tensile strength. The copolyesters prepared with H3PO4/M(2-ethylhexanoate)2 displayed a less random distribution of –O2CC6H4CO2– and –OCH2CH2O– units, leading to a faster crystallization rate, higher Tm value, and higher yield strength compared to those prepared with (nBuO)4Ti using the same amount of BHET. Furthermore, they displayed substantial shear-thinning behavior in their rheological properties due to the presence of long-chain branches of (–CH2O)3P=O units. Unfortunately, the copolyesters prepared with H3PO4/M(2-ethylhexanoate)2, and hence containing M2+, –CH2OP(O)(OH)O−, (–CH2O)2P(O)O− groups, did not exhibit enhanced biodegradability under ambient soil conditions.

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“…Microorganisms offer numerous advantages over higher eukaryotic organisms for FFA production, including a simpler metabolism, being more amenable to genetic engineering, and demonstrating faster growth rates and safe production, making them well-suited for large-scale industrial applications. Furthermore, the enhanced accessibility of reaction/pathway and omics databases, genetic engineering tools, and high-throughput screening methods has facilitated the development of synthetic microbial cell factories proficient in the synthesis of diverse industrially relevant compounds with remarkable efficiency [7,8]. Nonetheless, the efficient microbial production of FFAs remains a major challenge due to the tight control and regulation of FA metabolism, as most native microorganisms do not support the biosynthesis of excess FAs beyond their metabolic demands through a set of regulatory mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Tuning Fatty Acid Profile and Yield in Pichia pastoris

Kobalter,

Voit,

Bekerle-Bogner

et al. 2023

Bioengineering

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Fatty acids have been supplied for diverse non-food, industrial applications from plant oils and animal fats for many decades. Due to the massively increasing world population demanding a nutritious diet and the thrive to provide feedstocks for industrial production lines in a sustainable way, i.e., independent from food supply chains, alternative fatty acid sources have massively gained in importance. Carbohydrate-rich side-streams of agricultural production, e.g., molasses, lignocellulosic waste, glycerol from biodiesel production, and even CO2, are considered and employed as carbon sources for the fermentative accumulation of fatty acids in selected microbial hosts. While certain fatty acid species are readily accumulated in native microbial metabolic routes, other fatty acid species are scarce, and host strains need to be metabolically engineered for their high-level production. We report the metabolic engineering of Pichia pastoris to produce palmitoleic acid from glucose and discuss the beneficial and detrimental engineering steps in detail. Fatty acid secretion was achieved through the deletion of fatty acyl-CoA synthetases and overexpression of the truncated E. coli thioesterase ‘TesA. The best strains secreted >1 g/L free fatty acids into the culture medium. Additionally, the introduction of C16-specific ∆9-desaturases and fatty acid synthases, coupled with improved cultivation conditions, increased the palmitoleic acid content from 5.5% to 22%.

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Recent advances in the production of platform chemicals using metabolically engineered microorganisms

Cited by 8 publications

References 52 publications

Genetic engineering for biohydrogen production from microalgae

Genetic engineering for biohydrogen production from microalgae

Ductile Copolyesters Prepared Using Succinic Acid, 1,4-Butanediol, and Bis(2-hydroxyethyl) Terephthalate with Minimizing Generation of Tetrahydrofuran

Tuning Fatty Acid Profile and Yield in Pichia pastoris

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