Recent advances in the pretreatment of microalgal and lignocellulosic biomass: A comprehensive review

Sankaran, Revathy; Parra-Cruz, Ricardo; Pakalapati, Harshini; Show, Pau Loke; Ling, Tau Chuan; Chen, Wei‐Hsin; Tao, Yang

doi:10.1016/j.biortech.2019.122476

Cited by 221 publications

(71 citation statements)

References 130 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Comparing to chemical pretreatment, biological pretreatment utilizing enzyme has massive research potential given the advantages such as low energy consumption, environmentally friendliness, and lower production cost. Different lignocellulosic biomass needs different types of pretreatments because the structural features of cellulose plays an important role in enzyme hydrolysis rate that affects outcomes such as polymerization degree, cellulose crystallization arrangement, surface area accessibility, particles size, and the existence of hemicelluloses and lignin [2,43]. We show that TlSWO directly caused the alteration of the cellulose structure, consequentially increasing its hydrolysis rate.…”

Section: Discussionmentioning

confidence: 84%

A novel Swollenin from Talaromyces leycettanus JCM12802 enhances cellulase hydrolysis towards various substrates

Zhang

Wang

Brunecky

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Swollenins exist within some fungal species such as Trichoderma reesei and Aspergillus niger, and are candidate accessory enzymes for the biodegradation of cellulosic substrates. Swollenins theorized role in biological processes is as promoters in the rearrangement of non-covalent interaction networks formed by cell wall polysaccharides. Such network rearrangements though to be responsible for expanding accessibility for hydrolytic enzyme degradation processes. We hereby report in detail the characterization of a recombinant swollenin and its disruptive activity on cellulosic substrates and its synergistic effects while interacting with cellulases.Results: We have expressed a novel swollenin gene Tlswo consists of an open reading frame encoding 503 amino acids to be identified from Talaromyces leycettanus JCM12802 and successfully expressed it in both Trichoderma reesei and Pichia pastoris. Similar to other fungal swollenins, TlSWO is composed of a N-terminal family 1 carbohydrate binding module (CBM1) followed by a Ser/Thr rich linker connected to an expansin-like domain, followed by a family of 45 endoglucanase-like domains and a group-2 grass pollen allergen domain. From our experimental results, TlSWO demonstrated disruptive activity on Avicel and displayed a synergistic effect with cellobiohydrolases, enhancing its hydrolytic performance up to 132%. We also explored the activity of TlSWO on several model substrates as well as pretreated biomass. Our results indicate that TlSWO is capable of releasing reducing sugars from lichenan, barley β-glucan, carboxymethyl cellulose sodium (CMC-Na) and laminarin. The specific activity of TlSWO towards the substates mentioned above is 9.0 ± 0.100 U/mg, 8.9 ± 0.100U/mg, 2.3 ± 0.002 U/mg and 0.79 ± 0.002 U/mg, respectively. Moreover, further experimentation confirms the fungal swollenin TlSWO exhibits maximum activity at pH 4.0 and 50 ℃.Conclusion: This study reports on a novel swollenin protein capable of enhancing biomass conversion. Experimental data reveals the functional diversity of this swollenin with considerable activity on various substrates. Although the exact catalytic mechanism of swollenin’s remains unknown, the functional diversity of TlSWO broadens its applicability under experimental settings, and suggests it may be a promising candidate for future industrial applications.

show abstract

Section: Discussionmentioning

confidence: 84%

A novel Swollenin from Talaromyces leycettanus JCM12802 enhances cellulase hydrolysis towards various substrates

Zhang

Wang

Brunecky

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…The technical challenge of lignocellulose biomass remains at the recalcitrance structure which composes of interwoven polymetric components (ie, cellulose, hemicellulose, and lignin). Many review studies have reviewed various technologies to overcome the challenges regarding the pre‐treatment process of lignocellulose biomass 91‐93 . Aside from that, the biofuel production cost is estimated to be around US$1.65/gal, which is two to three times higher than petroleum fuels 94 .…”

Section: Second Generation Biofuelsmentioning

confidence: 99%

“…One of the main challenges in utilizing the second generation biofuels is the high energy consumption during the process of biofuel conversion. Second generation biomass requires a pre‐treatment step to break down the rigid recalcitrance structure of the lignocellulose biomass and this influences the cost difference between the first and second generation biofuels 92,95 . An evaluation of the cost reduction should be done for each of the pre‐treatment steps to improve the overall production process.…”

Section: Second Generation Biofuelsmentioning

confidence: 99%

Sustainability of the four generations of biofuels – A review

et al. 2020

Self Cite

View full text Add to dashboard Cite

Biofuel has emerged as an alternative source of energy to reduce the emissions of greenhouse gases in the atmosphere and combat global warming. Biofuels are classified into first, second, third and fourth generations. Each of the biofuel generations aims to meet the global energy demand while minimizing environmental impacts. Sustainability is defined as meeting the needs of the current generations without jeopardizing the needs of future generations. The aim of sustainability is to ensure continuous growth of the economy while protecting the environment and societal needs. Thus, this paper aims to evaluate the sustainability of these four generations of biofuels. The objectives are to compare the production of biofuel, the net greenhouse gases emissions, and energy efficiency. This study is important in providing information for the policymakers and researchers in the decision-making for the future development of green energy. Each of the biofuel generations shows different benefits and drawbacks. From this study, we conclude that the first generation biofuel has the highest biofuel production and energy efficiency, but is less effective in meeting the goal of reducing the greenhouse gases emission. The third generation biofuel shows the lowest net greenhouse gases emissions, allowing the reduction of greenhouse gases in the atmosphere. However, the energy required for the processing of the third generation biofuel is higher and, this makes it less environmentally friendly as fossil fuels are used to generate electricity. The third and fourth generation feedstocks are the potential sustainable source for the future production of biofuel. However, more studies need to be done to find an alternative low cost for biofuel production while increasing energy efficiency.

show abstract

“…Direct solvent extraction or other methods to extract oil are also less efficient. Microalgae cell crushing methods are comprised of mechanical and non-mechanical methods (Supplementary Figure S4) (Grimi et al, 2014;Günerken et al, 2015;Onumaegbu et al, 2018;Goh et al, 2019;Hu Y. et al, 2019;Li X. et al, 2019;Mathimani and Mallick, 2019;bin Azmi et al, 2020;Katiyar and Arora, 2020;Sankaran et al, 2020). Commonly used mechanical cells broken pretreatment methods are mainly physical methods, such as ball mill, homogeneous and cavitation, but non-mechanical methods include chemical method and biological method.…”

Section: Extraction and Preparation Of Edible Microalgae Oil Cell Dismentioning

confidence: 99%

Development Prospect and Preparation Technology of Edible Oil From Microalgae

Xue

et al. 2020

Front. Mar. Sci.

View full text Add to dashboard Cite

Microalgae are a valuable and rich source of oil. By the means of using microalgae oil as raw materials or additives for edible oil will improve the nutritional and edible value of the latter. This paper compares the lipid content and fatty acid composition of microalgae and traditional oil crops, elaborates the lipid synthesis mechanism of microalgae cells and the strategies of promoting lipid accumulation both from environmental pressure and from molecular pressure, summarizes the methods of breaking microalgae cell walls and extracting oil, analyzes the nutritional value, toxicity, antioxidant, and economic feasibility issues to be considered in microalgae edible oil. It is hoped that this study could provide theoretical basis for the actual production of microalgae edible oil, and provide inspiration for the research on the synthesis, accumulation, extraction and preparation of microalgae oils.

show abstract

Recent advances in the pretreatment of microalgal and lignocellulosic biomass: A comprehensive review

Cited by 221 publications

References 130 publications

A novel Swollenin from Talaromyces leycettanus JCM12802 enhances cellulase hydrolysis towards various substrates

A novel Swollenin from Talaromyces leycettanus JCM12802 enhances cellulase hydrolysis towards various substrates

Sustainability of the four generations of biofuels – A review

Development Prospect and Preparation Technology of Edible Oil From Microalgae

Contact Info

Product

Resources

About