Recent advances in the encapsulation of bioactive ingredients using galactomannans-based as delivery systems

Santos, Monique Barreto; Garcia‐Rojas, Edwin Elard

doi:10.1016/j.foodhyd.2021.106815

Cited by 27 publications

(14 citation statements)

References 57 publications

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“…Pectinase enzymes that could work at high temperatures and suitable pHs conditions speed up the previous processes. Speed of processing shows an economic gain in industrial and commercial applications [4]. To further purify the enzyme, a combination of one or more techniques is applied, viz.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Aspergillus niger NRC1ami Pectinase Using Citrus Peel Pectin, Purification, and Thermodynamic Characterization of the Free and Modified Enzyme

Esawy

Gamal

Kamel

2022

Waste Biomass Valor

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Enzyme cost and stability are the main problems facing industrial applications. Consequently, Aspergillus nigerNRC1ami was isolated from rotten orange and recorded a promising pectinase activity (13.8 U/ml). The enzyme was optimized using citrus peel pectin as the sole carbon source and recorded (40 U/ml). It was purified by two steps purifications and recorded 632 purification folds. The pure enzyme showed 14.7% carbohydrate content and consists of 15 amino acids. Glutamic acid was the major (22%) followed by leucine (10.67%) and threonine was the minor (2.70%). A. niger NRC1ami pectinase was conjugated by covalent coupling to sodium periodate (NaIO4) activated polysaccharides. Galactomannan showed the highest recovered activity (85%) and 94.34% reduction in viscosity. The optimum temperature for the pure enzyme shifted from 40 to 45 °C after the conjugation process. Also, the free enzyme showed its optimum activity at pH 5 compared to pH 4, 5 in the conjugated form case. The thermal stability of the free enzyme greatly improved after the modification process. The conjugated process reduced the activation energy to 36%, prolonged the enzyme half-life 5.6-fold at 60 °C, and increase the deactivation energy (Ed) by about 19% in comparison to the free form. The D value of the conjugated enzyme increased to 13.2-fold at 50 °C compared to the free form. Gibbs's free energy (ΔG) of the enzyme increased after the modification process, while the enthalpy (ΔH) and entropy (ΔS) decreased. Na+ and Zn2+ had a stimulating effect on both enzyme forms. Graphical Abstract

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Section: Introductionmentioning

confidence: 99%

Optimization of Aspergillus niger NRC1ami Pectinase Using Citrus Peel Pectin, Purification, and Thermodynamic Characterization of the Free and Modified Enzyme

Esawy

Gamal

Kamel

2022

Waste Biomass Valor

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“…HSPS is significantly better than HSPP in scavenging ABTS, superoxide ions, and hydroxyl radicals. Though present research data suggested that carboxymethylated polysaccharides were generally nontoxic in cell and animal models (Santos & Garcia-Rojas, 2021;Wang et al, 2015). carboxymethylation and phosphorylation are chemical modification, and are still worth considering whether all carboxymethylated and phosphorylated polysaccharides together with their products are nontoxic and safe.…”

Section: Discussionmentioning

confidence: 74%

Preparation and application of carboxymethylated and phosphatised Melaleuca polysaccharide

Yan

Yuan

et al. 2023

Food Sci. Technol

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Polysaccharides are a class of biological macromolecules made up of sugar chains with multiple glycosidic bonds. To explore the extracted, purified, carboxymethylated and phosphorylated modified to obtained carboxymethylated polysaccharides (HSPS) and phosphorylated polysaccharides (HSPP), and the HSPS and HSPP were characterized and analyzed, and their antioxidant activity measured. Response surface design was used for the synthesis process of HSPS and HSPP, the structure characterization and antioxidant activities of HSPS and HSPP were evaluated. The results showed that the Mw and Mn of HSP were 14.66 kDa and 8.39 kDa, respectively. The monosaccharides were mainly galactose, glucose, xylose, and arabinose in a molar ratio of 37.68%: 24.43%: 13.63%: 9.18%. The optimal synthesis conditions for HSPS were as follows: reaction time of 2.01 h, reaction temperature of 44.61°C and the amount of monochloroacetic acid necessary was 1.18 g. The optimal synthesis conditions for HSPP were: reaction time of 4.28 h, reaction temperature of 80.78°C, and reaction pH of 8.98. HSPS and HSPP have scavenging effects on ABTS, super oxideanion and hydroxyl radicals. The HSPS and HSPP were characterized and analyzed, and their antioxidant activity measured.

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“…As specified by Barreto Santos et al and by Yadav and Maiti, galactomannan polysaccharides can easily be chemically derivatized by functions such as sulfation, carboxylation, or acetylation, for example [ 13 , 144 ]. This derivatization usually employs hazardous chemicals and solvents but produces non-toxic and biodegradable matrices.…”

Section: Lbg Derivativesmentioning

confidence: 99%

Locust Bean Gum, a Vegetable Hydrocolloid with Industrial and Biopharmaceutical Applications

Petitjean

Isasi

2022

Molecules

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Locust bean gum (LBG), a vegetable galactomannan extracted from carob tree seeds, is extensively used in the food industry as a thickening agent (E410). Its molecular conformation in aqueous solutions determines its solubility and rheological performance. LBG is an interesting polysaccharide also because of its synergistic behavior with other biopolymers (xanthan gum, carrageenan, etc.). In addition, this hydrocolloid is easily modified by derivatization or crosslinking. These LBG-related products, besides their applications in the food industry, can be used as encapsulation and drug delivery devices, packaging materials, batteries, and catalyst supports, among other biopharmaceutical and industrial uses. As the new derivatized or crosslinked polymers based on LBG are mainly biodegradable and non-toxic, the use of this polysaccharide (by itself or combined with other biopolymers) will contribute to generating greener products, considering the origin of raw materials used, the modification procedures selected and the final destination of the products.

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Recent advances in the encapsulation of bioactive ingredients using galactomannans-based as delivery systems

Cited by 27 publications

References 57 publications

Optimization of Aspergillus niger NRC1ami Pectinase Using Citrus Peel Pectin, Purification, and Thermodynamic Characterization of the Free and Modified Enzyme

Optimization of Aspergillus niger NRC1ami Pectinase Using Citrus Peel Pectin, Purification, and Thermodynamic Characterization of the Free and Modified Enzyme

Preparation and application of carboxymethylated and phosphatised Melaleuca polysaccharide

Locust Bean Gum, a Vegetable Hydrocolloid with Industrial and Biopharmaceutical Applications

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