Recent advances in the biochemistry and physiology of the insulin-like growth factor/somatomedin family

Herington, Adrian C.; Cornell, Hugh J.; Kuffer, A.D.

doi:10.1016/0020-711x(83)90208-2

Cited by 58 publications

(18 citation statements)

References 92 publications

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“…This hypothesis has evolved to include the concept that somatomedins are produced primarily by the liver in response to GH (2)(3)(4). Although most of the early evidence in support of this theory was derived from in vitro work, Schoenle et al (5) recently demonstrated a dose-related growth-promoting effect of systemically infused human somatomedin (hSm) on the proximal tibial epiphyseal plate of hypophysectomized rats.…”

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confidence: 99%

Evidence suggesting that the direct growth-promoting effect of growth hormone on cartilage in vivo is mediated by local production of somatomedin.

Schlechter¹,

Russell²,

Spencer³

et al. 1986

Proc. Natl. Acad. Sci. U.S.A.

291

125

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We have used a catheterization system that permits chronic infusion into the arterial supply of one hindlimb of rats to study the direct effects of rat growth hormone and human somatomedin C on growth of the tibial epiphyseal cartilage plate in hypophysectomized rats. Rat growth hormone (0.4 ,.g per day) or human somatomedin C (0.25, 1, or 4 ,ug per day) stimulated growth of the epiphyseal plate of the infused limb but not of that of the contralateral noninfused limb. The somatomedin C had a dose-related effect. Rabbit antiserum to human somatomedin C, but not normal rabbit serum, completely abolished the direct growth effect of the rat growth hormone when it was co-infused with the hormone. These results support the concept that growth hormone stimulates long bone growth by inducing local production of somatomedin, which in turn stimulates cell proliferation in an autocrine or paracrine fashion. However, they do not exclude the possibility that serum somatomedin may also play a role.The somatomedin hypothesis of Salmon and Daughaday (1) maintains that the growth-promoting actions of growth hormone (GH) are indirect and act via GH-dependent plasma factors named somatomedins or insulin-like growth factors. This hypothesis has evolved to include the concept that somatomedins are produced primarily by the liver in response to GH (2-4). Although most of the early evidence in support of this theory was derived from in vitro work, Schoenle et al. (5) recently demonstrated a dose-related growth-promoting effect of systemically infused human somatomedin (hSm) on the proximal tibial epiphyseal plate of hypophysectomized rats. However, a local effect of GH on tibial cartilage growth has also been demonstrated in hypophysectomized rats in vivo by direct injection into the proximal tibial epiphyseal plate (6, 7), by intraarticular injection into the knee joint or administration into the epiphysis by implanted cannula (8), and by chronic infusion into the arterial supply of one hindlimb (9). In addition, numerous cells and tissues have been shown to produce somatomedins in vitro, either spontaneously or in response to GH (10-18). Thus, it is possible that GH exerts its direct effects by stimulating local production of somatomedins.To investigate this possibility we used the system of Schlechter et al. (9), which permits chronic infusion into the arterial supply of one hindlimb of a rat. With this procedure, we have demonstrated a direct growth-promoting effect of human somatomedin C (hSm-C) on the proximal tibial epiphyseal plate of hypophysectomized rats. In addition, we have found that the local effect of rat GH (rGH) could be completely abolished by co-infusing an antiserum raised against hSm-C. MATERIALS AND METHODSMale Long-Evans rats (125-150 g) were obtained from our breeding colony. Husbandry conditions were as described (9). The animals were hypophysectomized by the transauricular approach (19) 14 days prior to catheterization. On the day of catheterization, they were reanesthetized (9) and the right superi...

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mentioning

confidence: 99%

Evidence suggesting that the direct growth-promoting effect of growth hormone on cartilage in vivo is mediated by local production of somatomedin.

Schlechter¹,

Russell²,

Spencer³

et al. 1986

Proc. Natl. Acad. Sci. U.S.A.

291

125

View full text Add to dashboard Cite

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“…The importance of synthetic oligodeoxyribonucleotides in molecular biology (1)(2)(3)(4)(5) has long attracted attention to the I refining of chemical synthetic methods. The most important aspect of chemical syntheses of oligodeoxyribonucleotides is the selective protection of different nucleophilic sites of the monomer building blocks, and amino protection is one of them.…”

Section: Introductionmentioning

confidence: 99%

“…On n'a pas observC d'effets contraires ni sur la liaison glycosidique (dipurination) ni sur la liaison intemucliotidique lors de la diprotection. On a caractCrisC les unitis monornkres par ultraviolet, par risonance magnitique nuclCaire et par analyse ClCmentaire tandis que l'hydrolyse enzymatique par le phosphodiestirase du venin de serpent suivie d'une phosphatase alcaline ont permis de caractiriser les titramkres.[Traduit par la revue] Introduction The importance of synthetic oligodeoxyribonucleotides in molecular biology (1)(2)(3)(4)(5) has long attracted attention to the …”

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Naphthaloyl group: a new selective amino protecting group for deoxynucleosides in oligonucleotide synthesis

Dikshit¹,

Chaddha²,

Singh³

et al. 1988

Can. J. Chem.

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ARCHANA DIKSHIT, MANJULA CHADDHA, R. K. SINGH, and KRISHNA MISRA. Can. J . Chem. 66, 2989 (1988). The naphthaloyl group has been found to be a selective amino protecting group for deoxycytidine, deoxyadenosine, and deoxyguanosine in oligodeoxyribonucleotide synthesis. All three protected monomers obtained (78-85%), being six-membered cyclic imides, were fairly stable. These protected monomers were used successfully for the preparation of dimers (phosphodiester approach) and tetramers (phosphotriester approach) in solution as well as solid phase, respectively. The group acted as a purification tool due to its high lipophilicity. No adverse effect has been observed either on the glycosidic bond (depurination) or the intemucleotidic bond during its removal. The monomeric units were characterized by UV, NMR, and elemental analyses whereas the tetramers were characterized by enzymatic hydrolyses with snake venom phosphodiesterase followed by alkaline phosphatase.ARCHANA DIKSHIT, MANJULA CHADDHA, R. K. SINGH, et KRISHNA MISRA. Can. J . Chem. 66, 2989 (1988). On a trouvC que le groupe naphtaloyle constitue un groupe protecteur selectif de la fonction amine de la dCoxycytidine, de la dCoxyadCnosine et de la dkoxyguanosine dans la synthkse de 1'oligodCoxyribonuclCotide. Les trois monomkres protCgCs obtenus (78-85%) sont des imides cycliques i six chainons assez stables. On a~u t i l i s~ avantageusement ces trois monomkres protCgCs dans la prkparation des dimkres (approche phosphodiester) et des titramkres (approche phosphotriesters) en solution aussi bien qu'en phase solide respectivement. La grande IipophilicitC de ce groupe en fait un outil de purification. On n'a pas observC d'effets contraires ni sur la liaison glycosidique (dipurination) ni sur la liaison intemucliotidique lors de la diprotection. On a caractCrisC les unitis monornkres par ultraviolet, par risonance magnitique nuclCaire et par analyse ClCmentaire tandis que l'hydrolyse enzymatique par le phosphodiestirase du venin de serpent suivie d'une phosphatase alcaline ont permis de caractiriser les titramkres.[Traduit par la revue] Introduction The importance of synthetic oligodeoxyribonucleotides in molecular biology (1)(2)(3)(4)(5) has long attracted attention to the

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“…Έχει βρεθεί ότι ο IGF-Ι είναι ο πιο ισχυρός (πολυπεπτίδιο 70 αμινοξέων) παρόλο που ο IGF-ΙΙ είναι ο πιο άφθονος αυξητικός παράγοντας στα οστά. Οι IGFs συνδέονται με υποδοχείς τύπου τυροσίνης -κινάσης (IGFRs) 71 . Η σύνδεση του IGF-Ι με τον υποδοχέα IGF τύπου Ι (IGFΙR) ενεργοποιεί σηματοδοτικά μονοπάτια κατά την ενδοκυττάρια μεταγωγή του σήματος, τα οποία προκαλούν μεταβολές στην έκφραση των γονιδίων και επάγουν θετικά τη ρύθμιση της αναβολικής απάντησης στο κύτταρο 72 .…”

Section: ινσουλινοειδής αυξητικός παράγοντας-ι (Igf-ι)unclassified

Εφαρμογή Συστήματος Διοστικών Υπέρηχων Στη Διεπιφάνεια Οστού-Τένοντα Και Στα Κατάγματα Μακρών Οστών. Πειραματική Και Κλινική Μελέτη

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Recent advances in the biochemistry and physiology of the insulin-like growth factor/somatomedin family

Cited by 58 publications

References 92 publications

Evidence suggesting that the direct growth-promoting effect of growth hormone on cartilage in vivo is mediated by local production of somatomedin.

Evidence suggesting that the direct growth-promoting effect of growth hormone on cartilage in vivo is mediated by local production of somatomedin.

Naphthaloyl group: a new selective amino protecting group for deoxynucleosides in oligonucleotide synthesis

Εφαρμογή Συστήματος Διοστικών Υπέρηχων Στη Διεπιφάνεια Οστού-Τένοντα Και Στα Κατάγματα Μακρών Οστών. Πειραματική Και Κλινική Μελέτη

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