Recent Advances in Single Cell Analysis Methods Based on Mass Spectrometry

Wang, Ju-Duo; Song, Jia-Feng; Li, Chang; Dai, Xinhua; Fang, Xiang; Gong, Xiaoyun; Ye, Zihong

doi:10.1016/s1872-2040(20)60038-x

Cited by 5 publications

(2 citation statements)

References 60 publications

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“…Cells are the foundational biological blocks that comprise every living entity. Due to the significant heterogeneity among cells, the analysis of individual organisms or biological tissues can hardly reflect the patterns of life activities at the level of individual cells ( Wang et al, 2020 ). To understand the essence of microscopic life activities more profoundly and acquire a more comprehensive and precise understanding of cytological information, related research must be conducted on a unicellular scale ( Zenobi, 2013 ; Xiong et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Recent developments in ionization techniques for single-cell mass spectrometry

Zeng,

Xia,

Yin

et al. 2023

Front. Chem.

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The variation among individual cells plays a significant role in many biological functions. Single-cell analysis is advantageous for gaining insight into intricate biochemical mechanisms rarely accessible when studying tissues as a whole. However, measurement on a unicellular scale is still challenging due to unicellular complex composition, minute substance quantities, and considerable differences in compound concentrations. Mass spectrometry has recently gained extensive attention in unicellular analytical fields due to its exceptional sensitivity, throughput, and compound identification abilities. At present, single-cell mass spectrometry primarily concentrates on the enhancement of ionization methods. The principal ionization approaches encompass nanoelectrospray ionization (nano-ESI), laser desorption ionization (LDI), secondary ion mass spectrometry (SIMS), and inductively coupled plasma (ICP). This article summarizes the most recent advancements in ionization techniques and explores their potential directions within the field of single-cell mass spectrometry.

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Section: Introductionmentioning

confidence: 99%

Recent developments in ionization techniques for single-cell mass spectrometry

Zeng,

Xia,

Yin

et al. 2023

Front. Chem.

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“…This mode has been employed with ablation techniques such as secondary ion mass spectrometry (SIMS) and laser ablation electrospray ionization (LAESI) [13,14]. Notably, dynamic SIMS operated in this mode allows subcellular 3D imaging by acquiring a series of images interleaved with etching [15,16]; however, data interpretation has proved challenging due to the low ion fluxes and fragmentation of biomolecules [17]. Additionally, depending on the nature of the primary ion beam used, it would take tens of layers ion beam ablation to sputter away a single cell, thus not practical for tissue imaging [18].…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional (3D) imaging of lipids in skin tissues with infrared matrix-assisted laser desorption electrospray ionization (MALDESI) mass spectrometry

2021

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Three-dimensional (3D) mass spectrometry imaging (MSI) has become a growing frontier as it has the potential to provide a 3D representation of analytes in a label-free, untargeted, and chemically specific manner. The most common 3D MSI is accomplished by the reconstruction of 2D MSI from serial cryosections; however, this presents significant challenges in image alignment and registration. An alternative method would be to sequentially image a sample by consecutive ablation events to create a 3D image. In this study, we describe the use of infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) in ablation-based 3D MSI for analyses of lipids within fresh frozen skin tissue. Depth resolution using different laser energy levels was explored with a confocal laser scanning microscope to establish the imaging parameters for skin. The lowest and highest laser energy level resulted in a depth resolution of 7 μm and 18 μm, respectively. A total of 594 lipids were putatively detected and detailed lipid profiles across different skin layers were revealed in a 56-layer 3D imaging experiment. Correlated with histological information, the skin structure was characterized with differential lipid distributions with a lateral resolution of 50 μm and a z resolution of 7 μm.

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