Recent advances in single-cell analysis by inductively coupled plasma-mass spectrometry: A review

Yu, Xiaoxiao; He, Man; Chen, Beibei; Hu, Bin

doi:10.1016/j.aca.2020.07.041

Cited by 41 publications

(23 citation statements)

References 103 publications

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“…Our results showed that the transport efficiency of the system for single cells was ~12%, which is better than many reports in the literature. 17 Under these circumstances, the optimal number of cells in suspension was ~10 5 mL -1 for SC-ICP-MS.…”

Section: Resultsmentioning

confidence: 99%

“…15 The above methods, however, are still difficult for the analysis of NPs in single cells mainly due to their high detection limits. Inductively coupled plasma mass spectrometry (ICP-MS), using a time-resolved mode, has already been developed for the analysis of intracellular elements, named single cell (SC)-ICP-MS. 16,17 Li et al studied the signals of 238 U in single Bacillus subtilis cells by time-resolved ICP-MS and found that the individual cells behave in ICP more like solid particles rather than wet droplets. 18 Ho et al determined the Mg content in individual algae cells by time-resolved ICP-MS. 19 Zheng et al determined quantum dots, Gd@C82(OH)22, and cisplatin with time-resolved ICP-MS and studied their uptake behavior at a single cell level.…”

Section: Introductionmentioning

confidence: 99%

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Quantitative Analysis of Gold Nanoparticles in Single Cells with Time-resolved ICP-MS

Liu¹,

Zheng²,

Shi³

et al. 2021

At.Spectrosc.

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Single-cell inductively coupled plasma mass spectrometry (SC-ICP-MS) has been introduced for the analysis of intracellular essential elements and nanoparticles (NPs) at the single cell level. However, it is still quite challenging for the accurate and reliable determination. In this work, a high-efficiency sample introduction system was used for single cell analysis with ICP-MS. The system includes a microconcentric nebulizer, a low-volume single pass spray chamber, and a syringe pump. The transport efficiency of single cells was greatly improved to ~12%. In addition, 197 Au signals in individual HepG2 cells, after incubation with gold nanoparticles (AuNPs) at the concentrations of 0.1, 0.5, and 1 μM for 12 h, were analyzed by time-resolved ICP-MS with dwell times of 100 μs and 5 ms, respectively. The 197 Au signal-tobackground ratio (S/B) at 100 μs dwell time was much higher than at 5 ms. For quantitative analysis, AuNP standard reference materials and Au standard solutions were used for calibration. The SC-ICP-MS data using NP calibration were in good agreement with those using solution ICP-MS analysis, validating the developed SC-ICP-MS method.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Quantitative Analysis of Gold Nanoparticles in Single Cells with Time-resolved ICP-MS

Liu¹,

Zheng²,

Shi³

et al. 2021

At.Spectrosc.

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“…Preparation. An appropriate amount of standard working solution of lamotrigine, oxcarbazepine, lacosamide, and topiramate was added to the rat plasma to prepare standard curves (5,20,100,500,1000,2000, and 5000 ng/mL of the standard solution). e quality control (QC) samples were prepared in the same way as the plasma standard curve to obtain low, medium, and high concentrations (10, 900, and 4500 ng/mL) solutions.…”

Section: Standard Curvementioning

confidence: 99%

“…e methods for the determination of antiepileptic drugs mainly include high-performance liquid chromatography (HPLC) [11][12][13][14], immunoassay [15,16], and gas chromatography-mass spectrometer (GC-MS) [17,18]. Liquid chromatography with tandem mass spectrometry (LC-MS/ MS) can obtain strong adduct ion peaks of the compounds under first-order mass spectrometry [19][20][21]. Although methods for measuring individual antiepileptic drug concentration have been widely reported, some patients with severe conditions require multiple drugs simultaneously.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Lamotrigine, Oxcarbazepine, Lacosamide, and Topiramate in Rat Plasma by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry

Qiu

Liang

et al. 2022

International Journal of Analytical Chemistry

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This study established an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method to study the pharmacokinetics of four antiepileptic drugs, lamotrigine, oxcarbazepine, lacosamide, and topiramate, in rats after oral administration. The gradient elution was performed on a UPLC HSS T3 (2.1 mm × 100 mm, 1.8 μm) column with acetonitrile-0.1% formic acid as the mobile phase at a flow rate of 0.4 mL/min. Protein precipitation by acetonitrile was adopted for plasma sample pretreatment. Electrospray- (ESI-) positive/negative ion switching and multiple reaction monitoring (MRM) modes were adopted for ion quantitative determination of antiepileptic drugs. UPLC-MS/MS detection and Drug and Statistics (DAS) software fitting were performed to blood samples collected from rats after oral administration of lamotrigine, oxcarbazepine, lacosamide, and topiramate (5 mg/kg). All drugs examined showed linearity within 5–5000 ng/ml (R2 > 0.9987), the intraday accuracy was within 92%–108%, and the interday accuracy was within 93%–109%. The relative standard deviations (RSD) of intraday and interday were less than 15%. The matrix effect was within 91%–105%, and the recovery was better than 88%. The established UPLC-MS/MS method was successfully applied to the pharmacokinetic study of lamotrigine, oxcarbazepine, lacosamide, and topiramate in rats.

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“…More recently, an ICP-OES method was also used for silver nanoparticle speciation after sequential extraction of the solid materials [ 30 ]. Comprehensive reviews and comparisons of different techniques and their applications in single-cell analyses have been reviewed extensively [ 13 , 14 , 31 – 35 ].…”

Section: Introductionmentioning

confidence: 99%

Quantification of silver nanoparticle interactions with yeast Saccharomyces cerevisiae studied using single-cell ICP-MS

et al. 2022

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Silver nanoparticles (AgNPs) have been used in many fields due to their anticancer, antimicrobial, and antiviral potential. Single-cell ICP-MS (SC-ICP-MS) is an emerging technology that allows for the rapid characterization and quantification of a metal analyte across a cell population in a single analysis. In this study, a new rapid and sensitive SC-ICP-MS method was developed to quantitatively study the interactions of AgNPs with yeast Saccharomyces cerevisiae . The method can quantify the cell concentration, silver concentration per cell, and profile the nanoparticle distribution in a yeast cell population. AgNP dosing time, concentration, and AgNP size were quantitatively evaluated for their effects on AgNP-yeast cell interactions. The results showed that the initial uptake of AgNPs was rapid and primarily driven by the mass of Ag per cell. The optimal dosing particle concentrations for highest uptake were approximately 1820, 1000, and 300 AgNPs/cell for 10, 20, and 40 nm AgNPs, respectively. Furthermore, this study also validated a washing method for the application to a microorganism for the first time and was used to quantitatively determine the amount of cell surface–adsorbed AgNPs and intracellular AgNPs. These results indicated that the mass (Ag in ag/cell) ratios of intracelluar vs cell surface-adsorbed AgNPs were similar for different AgNP sizes. This high throughput and ultrasensitive SC-ICP-MS method is expected to have many potential applications, such as optimization of methods for green synthesis of AgNPs, nanotoxicity studies, and drug delivery. This is the first quantification study on the interactions of AgNPs and S. cerevisiae using SC-ICP-MS. Graphical abstract Supplementary Information The online version contains supplementary material available at 10.1007/s00216-022-03937-4.

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Recent advances in single-cell analysis by inductively coupled plasma-mass spectrometry: A review

Cited by 41 publications

References 103 publications

Quantitative Analysis of Gold Nanoparticles in Single Cells with Time-resolved ICP-MS

Quantitative Analysis of Gold Nanoparticles in Single Cells with Time-resolved ICP-MS

Simultaneous Determination of Lamotrigine, Oxcarbazepine, Lacosamide, and Topiramate in Rat Plasma by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry

Quantification of silver nanoparticle interactions with yeast Saccharomyces cerevisiae studied using single-cell ICP-MS

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