Recent Advances in Scaffold Design and Material for Vascularized Tissue‐Engineered Bone Regeneration

Yin, Shi; Zhang, Wenjie; Zhang, Zhiyuan; Jiang, Xinquan

doi:10.1002/adhm.201801433

Cited by 201 publications

(139 citation statements)

References 120 publications

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“…EC-specific deletion of VEGFR2 results in reduction and disorganization of blood vessels in the metaphyseal region closed to the growth plate. (75) VEGFA exists in multiple isoforms-VEGF 120 , VEGF 164 , and VEGF 188 in mouse and VEGF 121 , VEGF 145 , VEGF 165 , VEGF 183 , VEGF 186 , VEGF 189 , and VEGF 206 in human, which elicit diverse biological processes. (76,77) Nevertheless, reduced endochondral angiogenesis and mineralization occurs in mice expressing only VEGF 120 isoform.…”

Section: Role Of Vegfa During Bone Developmentmentioning

confidence: 99%

“…Several strategies have been reported to enhance vascularization in bone tissue engineering, including the delivery of angiogenic growth factor, incorporation of optimal seed cells, and in vivo prevascularization approaches for bone regeneration ( Table 2). (188,189) Because of the coupling of angiogenesis and osteogenesis, angiogenic growth factors have gained great attention as a target for improving the success rate of bone tissue engineering. Most notably, the incorporation of VEGF from bone scaffolds can promote neovascularization and endochondral ossification as observed in a rat defect model in the presence of a bioactive glass coating with an additive VEGF release effect.…”

Section: Angiogenesis and Vascularization In Bone Tissue Engineeringmentioning

confidence: 99%

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Bone Vasculature and Bone Marrow Vascular Niches in Health and Disease

Chen

Hendriks

Chatzis

et al. 2020

Journal of Bone and Mineral Research

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Bone vasculature and bone marrow vascular niches supply oxygen, nutrients, and secrete angiocrine factors required for the survival, maintenance, and self-renewal of stem and progenitor cells. In the skeletal system, vasculature creates nurturing niches for bone and blood-forming stem cells. Blood vessels regulate hematopoiesis and drive bone formation during development, repair, and regeneration. Dysfunctional vascular niches induce skeletal aging, bone diseases, and hematological disorders. Recent cellular and molecular characterization of the bone marrow microenvironment has provided unprecedented insights into the complexity, heterogeneity, and functions of the bone vasculature and vascular niches. The bone vasculature is composed of distinct vessel subtypes that differentially regulate osteogenesis, hematopoiesis, and disease conditions in bones. Further, bone marrow vascular niches supporting stem cells are often complex microenvironments involving multiple different cell populations and vessel subtypes. This review provides an overview of the emerging vascular cell heterogeneity in bone and the new roles of the bone vasculature and associated vascular niches in health and disease.

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Section: Role Of Vegfa During Bone Developmentmentioning

confidence: 99%

Section: Angiogenesis and Vascularization In Bone Tissue Engineeringmentioning

confidence: 99%

Bone Vasculature and Bone Marrow Vascular Niches in Health and Disease

Chen

Hendriks

Chatzis

et al. 2020

Journal of Bone and Mineral Research

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“…On the other hand, of significant importance in the design of scaffolds are the final architecture, excellent mechanical properties, and biocompatibility, in addition to cell adhesion, proliferation, and differentiation abilities [2]. Chemical composition and structural modifications of the materials selected for scaffold design are the ultimate aspects to determine the success in tissue-engineering applications [3,4].…”

Section: Introductionmentioning

confidence: 99%

Synthesis, Characterization, and Histological Evaluation of Chitosan-Ruta Graveolens Essential Oil Films

et al. 2020

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The development of new biocompatible materials for application in the replacement of deteriorated tissues (due to accidents and diseases) has gained a lot of attention due to the high demand around the world. Tissue engineering offers multiple options from biocompatible materials with easy resorption. Chitosan (CS) is a biopolymer derived from chitin, the second most abundant polysaccharide in nature, which has been highly used for cell regeneration applications. In this work, CS films and Ruta graveolens essential oil (RGEO) were incorporated to obtain porous and resorbable materials, which did not generate allergic reactions. An oil-free formulation (F1: CS) and three different formulations containing R. graveolens essential oil were prepared (F2: CS-RGEO 0.5%; F3: CS+RGEO 1.0%; and F4: CS+RGEO 1.5%) to evaluate the effect of the RGEO incorporation in the mechanical and thermal stability of the films. Infrared spectroscopy (FTIR) analyses demonstrated the presence of RGEO. In contrast, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analysis showed that the crystalline structure and percentage of CS were slightly affected by the RGEO incorporation. Interesting saturation phenomena were observed for mechanical and water permeability tests when RGEO was incorporated at higher than 0.5% (v/v). The results of subdermal implantation after 30 days in Wistar rats showed that increasing the amount of RGEO resulted in greater resorption of the material, but also more significant inflammation of the tissue surrounding the materials. On the other hand, the thermal analysis showed that the RGEO incorporation almost did not affect thermal degradation. However, mechanical properties demonstrated an understandable loss of tensile strength and Young’s modulus for F3 and F4. However, given the volatility of the RGEO, it was possible to generate a slightly porous structure, as can be seen in the microstructure analysis of the surface and the cross-section of the films. The cytotoxicity analysis of the CS+RGEO compositions by the hemolysis technique agreed with in vivo results of the low toxicity observed. All these results demonstrate that films including crude essential oil have great application potential in the biomedical field.

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“…Various scaffolds have been investigated for bone tissue engineering (BTE) and synthesized using both inorganic and organic materials [38]. Generally, an ideal scaffold should have precise features in order to have an optimal integration and provide the correct stability [39].…”

Section: Scaffolds and Adipose Stem Cellsmentioning

confidence: 99%

Adipose-Derived Stem Cells in Bone Tissue Engineering: Useful Tools with New Applications

Storti

Scioli

Kim

et al. 2019

Stem Cells International

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Adipose stem cells (ASCs) are a crucial element in bone tissue engineering (BTE). They are easy to harvest and isolate, and they are available in significative quantities, thus offering a feasible and valid alternative to other sources of mesenchymal stem cells (MSCs), like bone marrow. Together with an advantageous proliferative and differentiative profile, they also offer a high paracrine activity through the secretion of several bioactive molecules (such as growth factors and miRNAs) via a sustained exosomal release which can exert efficient conditioning on the surrounding microenvironment. BTE relies on three key elements: (1) scaffold, (2) osteoprogenitor cells, and (3) bioactive factors. These elements have been thoroughly investigated over the years. The use of ASCs has offered significative new advancements in the efficacy of each of these elements. Notably, the phenotypic study of ASCs allowed discovering cell subpopulations, which have enhanced osteogenic and vasculogenic capacity. ASCs favored a better vascularization and integration of the scaffolds, while improvements in scaffolds' materials and design tried to exploit the osteogenic features of ASCs, thus reducing the need for external bioactive factors. At the same time, ASCs proved to be an incredible source of bioactive, proosteogenic factors that are released through their abundant exosome secretion. ASC exosomes can exert significant paracrine effects in the surroundings, even in the absence of the primary cells. These paracrine signals recruit progenitor cells from the host tissues and enhance regeneration. In this review, we will focus on the recent discoveries which have involved the use of ASCs in BTE. In particular, we are going to analyze the different ASCs' subpopulations, the interaction between ASCs and scaffolds, and the bioactive factors which are secreted by ASCs or can induce their osteogenic commitment. All these advancements are ultimately intended for a faster translational and clinical application of BTE.

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Recent Advances in Scaffold Design and Material for Vascularized Tissue‐Engineered Bone Regeneration

Cited by 201 publications

References 120 publications

Bone Vasculature and Bone Marrow Vascular Niches in Health and Disease

Bone Vasculature and Bone Marrow Vascular Niches in Health and Disease

Synthesis, Characterization, and Histological Evaluation of Chitosan-Ruta Graveolens Essential Oil Films

Adipose-Derived Stem Cells in Bone Tissue Engineering: Useful Tools with New Applications

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