Recent advances in research on vine tea, a potential and functional herbal tea with dihydromyricetin and myricetin as major bioactive compounds

Zhang, Qili; Zhao, Yanfang; Zhang, Meiyan; Zhang, Yalu; Ji, Hong‐Fang; Shen, Liang

doi:10.1016/j.jpha.2020.10.002

Cited by 49 publications

(37 citation statements)

References 98 publications

(140 reference statements)

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“…e results showed that DMY had a general inhibitory effect on all wild strains but a higher inhibition against P. antarctica with the MIC of 2.0 mg/mL and MBC of 6.4 mg/mL. e results indicated that DMY had a significant inhibitory effect on bacteria, which was consistent with the results of other researches [23,24].…”

Section: Mic and Mbcsupporting

confidence: 89%

Antibacterial Effect of Dihydromyricetin on Specific Spoilage Organisms of Hybrid Grouper

Huang

Xie

2021

Journal of Food Quality

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This study aimed to investigate the mechanism of antibacterial activity level inhibition of dihydromyricetin (DMY) against specific spoilage bacteria of grouper. Firstly, the specific spoilage bacteria of grouper in the cold storage process are Pseudomonas antarctica (P. antarctica), which are selected by calculating the spoilage metabolite yield factor. It was determined that the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of DMY against grouper spoilage bacteria were 2.0 mg/mL and 6.4 mg/mL, respectively. DMY was added to the matrix of chitosan and sodium alginate, and DMY emulsions of different concentrations (0 MIC, 1 MIC, 2 MIC, 4 MIC) were prepared and characterized by differential calorimetry methods. Through analyzing cell permeability, enzyme activity, and images of the confocal laser scanning microscope (CLSM), we further studied the antibacterial mechanism of DMY emulsion on specific spoilage bacteria. The results showed that, with the increase of DMY concentration in the treatment group, the leakage of nucleic acid and protein increased significantly, the activity of ATPase and three critical enzymes in the Embden-Meyerhof-Parnas (EMP) pathway decreased significantly, and the activity of AKPase did not decrease significantly, . The metabolic activity and viability are reduced considerably. Analysis of the above results shows that DMY inhibits the growth and reproduction of P. antarctica by interfering with the metabolic activity of bacteria and destroying the function of bacterial cell membranes but has no inhibitory effect on the activity of AKPase. This study proves that DMY could be an effective and natural antibacterial agent against specific spoilage bacteria in aquatic products.

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Section: Mic and Mbcsupporting

confidence: 89%

Antibacterial Effect of Dihydromyricetin on Specific Spoilage Organisms of Hybrid Grouper

Huang

Xie

2021

Journal of Food Quality

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“…Moreover, extracts from vine tea have been proved to have significant anti-inflammatory properties in vitro and in vivo , and have been recommended as a potential therapeutic agent for inflammation-related diseases ( Chen et al, 2015 ). Importantly, the leaves and stems of vine tea are rich in a large number of natural active substance dihydromyricetin, which has become an important plant resource for the development and research of functional products ( Gao et al, 2009 ; Kou and Chen, 2012 ; Zhao et al, 2013 ; Zheng et al, 2014 ; Ye et al, 2015 ; Zhang et al, 2020 ; Carneiro et al, 2021 ). It is worth mentioning that as a natural active substance, dihydromyricetin from vine tea has been approved by the Food and Drug Administration (FDA) as a nutritional supplement and can be added in food industry.…”

Section: Introductionmentioning

confidence: 99%

Dihydromyricetin Reverses Thioacetamide-Induced Liver Fibrosis Through Inhibiting NF-κB-Mediated Inflammation and TGF-β1-Regulated of PI3K/Akt Signaling Pathway

et al. 2021

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As a natural active substance, dihydromyricetin (DHM) has been proven to have good hepatoprotective activity. However, the therapeutic effect of DHM on liver fibrosis, which has become a liver disease threatening the health of people around the world, has not been studied to date. The purpose of this study was to investigate the effect of DHM as a new nutritional supplement on thioacetamide (TAA)-induced liver fibrosis. The liver fibrosis model was established by intraperitoneal injection of TAA (200 mg/kg, every 3 days) for 8 weeks, and oral administration of DHM (20 mg/kg and 40 mg/kg, daily) after 4 weeks of TAA-induced liver fibrosis. The results showed that DHM treatment significantly inhibited the activities of alanine aminotransferase (ALT) (37.81 ± 7.62 U/L) and aspartate aminotransferase (AST) (55.18 ± 10.94 U/L) in serum of liver fibrosis mice, and increased the levels of superoxide dismutase (SOD) and glutathione (GSH) while reversed the level of malondialdehyde (MDA). In addition, histopathological examination illustrated that TAA induced the inflammatory infiltration, apoptosis and fibroatherosclerotic deposition in liver, which was further confirmed by western-blot and immunofluorescence staining. Moreover, DHM inhibited hepatocyte apoptosis by regulating the phosphorylation level of phosphatidylinositol 3-kinase (PI3K), protein kinase-B (AKT) and its downstream apoptotic protein family. Interestingly, immunofluorescence staining showed that DHM treatment significantly inhibited alpha smooth muscle actin (α-SMA), which was a marker of hepatic stellate cell activation, and regulated the expression of transforming growth factor (TGF-β1). Importantly, supplementation with DHM significantly inhibited the release of nuclear factor kappa-B (NF-κB) signaling pathway and pro-inflammatory factors in liver tissue induced by TAA, and improved liver fiber diseases, such as tumor necrosis factor alpha (TNF-α) and recombinant rat IL-1β (IL-1β). In conclusion, the evidence of this study revealed that DHM is a potential hepatoprotective and health factor, and which also provides the possibility for the treatment of liver fibrosis.

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“…The six phytoconstituents were identified as follows: (1) myricetin (C 15 H 10 O 8 ), (2) myricetin 3‐O‐rhamnoside (C 21 H 20 O 12 ), (3) 5,7,8,3,4‐pentahydroxyisoflavone (C 15 H 10 O 7 ), (4) dihydroquercetin (C 15 H 12 O 7 ), (5) 6,8‐dihydroxykaempferol (C 15 H 10 O 8 ), and (6) ellagic acid glucoside (C 20 H 16 O 13 ) as mentioned in Table 1. Previous reports have published and reported some of these compounds from vine tea but not all six in the same plant, such as C 15 H 10 O 8 (Fan et al., 2012; Zhang et al., 2020; Zhao et al., 2021; Long et al., 2019), C 21 H 20 O 12 (Ma et al., 2020; Zhang et al., 2020), C 15 H 10 O 7 (Cui et al., 2021; Hosny & Rosazza, 1999), C 15 H 12 O 7 (Gao et al., 2017), C 15 H 10 O 8 (Kim et al., 2019; Zhou et al., 2013), and C 20 H 16 O 13 (Kuba‐Miyara et al., 2012; Yu et al., 2021). The chemical structure of all six isolated compounds is enlisted in Figure 2.…”

Section: Resultsmentioning

confidence: 99%

LC‐ESI‐QTOF/MS characterization of antimicrobial compounds with their action mode extracted from vine tea (Ampelopsis grossedentata) leaves

Umair

Sultana

Zhu

et al. 2022

Food Science & Nutrition

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Vine tea (Ampelopsis grossedentata) is a tea plant cultivated south of the Chinese Yangtze River. It has anti‐inflammatory properties and is used to normalize blood circulation and detoxification. The leaves of vine tea are the most abundant source of flavonoids, such as dihydromyricetin and myricetin. However, as the main bioactive flavonoid in vine tea, dihydromyricetin was the main focus of previous research. This study aimed to explore the antibacterial activities of vine tea against selected foodborne pathogens. The antimicrobial activity of vine tea extract was evaluated by the agar well diffusion method. Cell membrane integrity and bactericidal kinetics, along with physical damage to the cell membrane, were also observed. The extract was analyzed using a high‐performance liquid chromatography‐diode array detector (HPLC‐DAD), and the results were confirmed using a modified version of a previously published method that combined liquid chromatography and electrospray‐ionized quadrupole time‐of‐flight mass spectrometry (LC‐ESI‐QTOF/MS). Cell membrane integrity and bactericidal kinetics were determined by releasing intracellular material in suspension and monitoring it at 260 nm using an ultraviolet (UV) spectrophotometer. A scanning electron microscope (SEM) was used to detect morphological alterations and physical damage to the cell membrane. Six compounds were isolated successfully: (1) myricetin (C15H10O8), (2) myricetin 3‐O‐rhamnoside (C21H20O12), (3) 5,7,8,3,4‐pentahydroxyisoflavone (C15H10O7), (4) dihydroquercetin (C15H12O7), (5) 6,8‐dihydroxykaempferol (C15H10O8), and (6) ellagic acid glucoside (C20H16O13). Among these bioactive compounds, C15H10O7 was found to have vigorous antimicrobial activity against Bacillus cereus (AS11846) and Staphylococcus aureus (CMCCB26003). A dose‐dependent bactericidal kinetics with a higher degree of absorbance at optical density 260 (OD260) was observed when the bacterial suspension was incubated with C15H10O7 for 8 h. Furthermore, a scanning electron microscope study revealed physical damage to the cell membrane. In addition, the action mode of C15H10O7 was on the cell wall of the target microorganism. Together, these results suggest that C15H10O7 has vigorous antimicrobial activity and can be used as a potent antimicrobial agent in the food processing industry.

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Recent advances in research on vine tea, a potential and functional herbal tea with dihydromyricetin and myricetin as major bioactive compounds

Cited by 49 publications

References 98 publications

Antibacterial Effect of Dihydromyricetin on Specific Spoilage Organisms of Hybrid Grouper

Antibacterial Effect of Dihydromyricetin on Specific Spoilage Organisms of Hybrid Grouper

Dihydromyricetin Reverses Thioacetamide-Induced Liver Fibrosis Through Inhibiting NF-κB-Mediated Inflammation and TGF-β1-Regulated of PI3K/Akt Signaling Pathway

LC‐ESI‐QTOF/MS characterization of antimicrobial compounds with their action mode extracted from vine tea (Ampelopsis grossedentata) leaves

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