Recent advances in research for potential utilization of unexplored lichen metabolites

Kalra, Rishu; Conlan, Xavier A.; Goel, Mayurika

doi:10.1016/j.biotechadv.2022.108072

Cited by 5 publications

(1 citation statement)

References 171 publications

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“…With the development of bioinformatics and the applying next-generation sequencing data, there has indeed been more focus on natural product discovery based on genomics (Garima et al, 2022b ; Luo et al, 2022 ). Genome mining has become a powerful tool to discover compounds, identify cryptic biosynthetic gene clusters, characterize the potential biosynthetic pathways, and predict the skeletal structure of the relative products (Liu Q. et al, 2022 ; Liu T. et al, 2022 ; Kalra et al, 2023 ). An increasing understanding of high-quality genome sequencing and genome mining techniques coupled with the introduction of powerful computational toolkits facilitates the process of connecting these gene clusters with key compounds (Li et al, 2016 ).…”

Section: Strategy To Discover Lichen Natural Productsmentioning

confidence: 99%

Discovery and excavation of lichen bioactive natural products

et al. 2023

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Lichen natural products are a tremendous source of new bioactive chemical entities for drug discovery. The ability to survive in harsh conditions can be directly correlated with the production of some unique lichen metabolites. Despite the potential applications, these unique metabolites have been underutilized by pharmaceutical and agrochemical industries due to their slow growth, low biomass availability, and technical challenges involved in their artificial cultivation. At the same time, DNA sequence data have revealed that the number of encoded biosynthetic gene clusters in a lichen is much higher than in natural products, and the majority of them are silent or poorly expressed. To meet these challenges, the one strain many compounds (OSMAC) strategy, as a comprehensive and powerful tool, has been developed to stimulate the activation of silent or cryptic biosynthetic gene clusters and exploit interesting lichen compounds for industrial applications. Furthermore, the development of molecular network techniques, modern bioinformatics, and genetic tools is opening up a new opportunity for the mining, modification, and production of lichen metabolites, rather than merely using traditional separation and purification techniques to obtain small amounts of chemical compounds. Heterologous expressed lichen-derived biosynthetic gene clusters in a cultivatable host offer a promising means for a sustainable supply of specialized metabolites. In this review, we summarized the known lichen bioactive metabolites and highlighted the application of OSMAC, molecular network, and genome mining-based strategies in lichen-forming fungi for the discovery of new cryptic lichen compounds.

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Section: Strategy To Discover Lichen Natural Productsmentioning

confidence: 99%

Discovery and excavation of lichen bioactive natural products

et al. 2023

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Evaluation of Phenazine Derivatives from the Lichen-Associated Streptomyces flavidovirens as Potent Antineuroinflammatory Agents In Vitro and In Vivo

Gao,

Liu

et al. 2024

J. Nat. Prod.

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Eighteen nitrogen-containing compounds (1–18) were isolated from cultures of the lichen-associated Streptomyces flavidovirens collected from the Qinghai-Tibet Plateau, including seven phenazine derivatives with three new ones, named subphenazines A–C (2–4), two new furan pyrrolidones (8–9), and nine known alkaloids. The structures were elucidated by spectroscopic data analysis, and absolute configurations were determined by single-crystal X-ray diffraction and ECD calculations. The phenazine-type derivatives, in particular compound 3, exhibited significantly better antineuroinflammatory activity than other isolated compounds (8–18). Compound 3 inhibited the release of proinflammatory cytokines including IL-6, TNF-α, and PGE2, and the nuclear translocation of NF-κB; it also reduced the oxidative stress and activated the Nrf2 signaling pathway in LPS-induced BV2 microglia cells. In vivo anti-inflammatory activity in zebrafish indicated that 3 inhibited LPS-stimulated ROS generation. These findings suggested that compound 3 might be a potent antineuroinflammatory agent through the regulation of the NF-κB/Nrf2 signaling pathways.

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Chemical composition and antioxidant, enzyme inhibition and cytoprotective activity of two Antarctic lichens of the genus Psoroma (Pannariaceae)

Torres-Benítez,

Ortega-Valencia,

Hillmann-Eggers

et al. 2024

Natural Product Research

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Lichens contain different types of chemical compounds with multiple biological activities that demonstrate their potential pharmacological use. This research aims to report the metabolomic identification of the ethanolic extracts of P. antarcticum and P. hypnorum, their antioxidant, enzyme inhibitory, and their cytoprotection activity. Sixteen metabolites were identified in P. antarcticum and twelve in P. hypnorum; the extracts reported variable antioxidant activity with IC50 >350 µg/mL in DPPH·, values >18 µmol Trolox/g in ORAC and >40 µmol Trolox/g in FRAP and a phenolic compound content >10 mg GAE/g, as well as significant results in cholinesterases, α-glucosidase, pancreatic lipase, α-amylase, and tyrosinase enzyme inhibition activities with IC50 ranging from 18 to 510 µg/mL, and which were complemented by molecular docking experiments; also both extracts showed improved cytoprotection at the concentrations of 0.5 to 1.0 μg/mL. This study contributes to the knowledge of the chemical richness of Antarctic lichen extracts and their effectiveness in the evaluation of biological activities related to neurodegenerative diseases and metabolic syndrome. Experimental ChemicalsUltrapure water (˂5 μg/L TOC) (water purification system Mili-Q Merck Milli-pore, Chile) was used. For mass spectrometry analysis, HPLC-grade methanol and MS-grade formic acid were used, obtained from J.T. Baker

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Recent advances in research for potential utilization of unexplored lichen metabolites

Cited by 5 publications

References 171 publications

Discovery and excavation of lichen bioactive natural products

Discovery and excavation of lichen bioactive natural products

Evaluation of Phenazine Derivatives from the Lichen-Associated Streptomyces flavidovirens as Potent Antineuroinflammatory Agents In Vitro and In Vivo

Chemical composition and antioxidant, enzyme inhibition and cytoprotective activity of two Antarctic lichens of the genus Psoroma (Pannariaceae)

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