Recent Advances in Recognition Receptors for Electrochemical Biosensing of Mycotoxins—A Review

Kaur, Manpreet; Gaba, Jyoti; Singh, Komal; Bhatia, Yashika; Singh, Anoop; Singh, Narinder

doi:10.3390/bios13030391

Cited by 3 publications

(5 citation statements)

References 125 publications

(156 reference statements)

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“…Chemical and electrochemical biosensors, including immuno, electrochemical, and optical types, based on nanoparticles or not, are effective tools for OTA detection. In fact, antibodies, aptamers, imprinted polymers, peptides, and DNAzymes have been employed as selective recognition receptors for the electrochemical biosensing of mycotoxins, including OTA [160]. Nanoparticles are utilized in colorimetric, fluorometric, enzymatic, and electrochemical assays, contributing to the precision and sensitivity of mycotoxin detection [160,161].…”

Section: Ota Analytical Methodsmentioning

confidence: 99%

“…In fact, antibodies, aptamers, imprinted polymers, peptides, and DNAzymes have been employed as selective recognition receptors for the electrochemical biosensing of mycotoxins, including OTA [160]. Nanoparticles are utilized in colorimetric, fluorometric, enzymatic, and electrochemical assays, contributing to the precision and sensitivity of mycotoxin detection [160,161]. Biosensors are easy to use, require a minimal volume of sample, and are characterized by fast response, a limit of detection (LOD) up to 0.31 fg/mL, and a linear range of 1-50 ng/mL [162], or an LOD of 0.18 ng/mL and a linear range of 0.78-200 ng/mL for OTA [163].…”

Section: Ota Analytical Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Comprehensive Insights into Ochratoxin A: Occurrence, Analysis, and Control Strategies

Ben Miri,

Benabdallah,

Chentir

et al. 2024

Foods

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Ochratoxin A (OTA) is a toxic mycotoxin produced by some mold species from genera Penicillium and Aspergillus. OTA has been detected in cereals, cereal-derived products, dried fruits, wine, grape juice, beer, tea, coffee, cocoa, nuts, spices, licorice, processed meat, cheese, and other foods. OTA can induce a wide range of health effects attributable to its toxicological properties, including teratogenicity, immunotoxicity, carcinogenicity, genotoxicity, neurotoxicity, and hepatotoxicity. OTA is not only toxic to humans but also harmful to livestock like cows, goats, and poultry. This is why the European Union and various countries regulate the maximum permitted levels of OTA in foods. This review intends to summarize all the main aspects concerning OTA, starting from the chemical structure and fungi that produce it, its presence in food, its toxicity, and methods of analysis, as well as control strategies, including both fungal development and methods of inactivation of the molecule. Finally, the review provides some ideas for future approaches aimed at reducing the OTA levels in foods.

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Section: Ota Analytical Methodsmentioning

confidence: 99%

Section: Ota Analytical Methodsmentioning

confidence: 99%

Comprehensive Insights into Ochratoxin A: Occurrence, Analysis, and Control Strategies

Ben Miri,

Benabdallah,

Chentir

et al. 2024

Foods

View full text Add to dashboard Cite

show abstract

“…Graphene-coated electrodes represent effective electrochemical transducers. Chronoamperometry allows fast assay, and it is considered as appropriate for assessing the repeatability and stability of electrochemical biosensors [190]. Majer-Baranyi and collaborators [191] also emphasize that electrochemical biosensors are the most broadly used due to the selectivity and sensitivity of the determinations and the advantages imparted by low cost, miniaturization, simplicity, and portability.…”

Section: Comparative Overview and Future Perspectivementioning

confidence: 99%

“…As in the case of other potential step techniques, one shortcoming is the duration of the experiment, which is reported as being longer than in amperometry or ramping techniques such as cyclic voltammetry [201]. Given the low detection limits imparted and excellent sensitivity, the square-wave voltammetric technique is reported as prone to simultaneous multiple analyte biosensing [190]. Nevertheless, some authors consider that electrochemical sensors, mostly those using aptamers as bioelements, though simple, sensitive, fast, and cost-effective, still require signal amplification protocols for sensitivity increase.…”

Section: Comparative Overview and Future Perspectivementioning

confidence: 99%

Biosensors for Food Mycotoxin Determination: A Comparative and Critical Review

Pisoschi,

Iordache,

Stanca

et al. 2024

Chemosensors

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The need for performant analytical methodologies to assess mycotoxins is vital, given the negative health impact of these compounds. Biosensors are analytical devices that consist of a biological element for recognizing the analyte and a transducer, which translates the biorecognition event into a signal proportional to the analyte concentration. The biorecognition elements can be enzymes, antibodies, or DNA fragments. The modalities of detection can be optical, electrochemical, thermal, or mass-sensitive. These analytical tools represent viable alternatives to laborious, expensive traditional methods and are characterized by specificity given by the biorecognition element, sensitivity, fast response, portability, multi-modal detection, and the possibility of in situ application. The present paper focuses on a comprehensive view, enriched with a critical, comparative perspective on mycotoxin assay using biosensors. The use of different biorecognition elements and detection modes are discussed comparatively. Nanomaterials with optical and electrochemical features can be exploited in association with a variety of biorecognition elements. Analytical parameters are reviewed along with a broad range of applications.

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“…The core principle of fabricated biosensors for mycotoxin detection relies on the dynamic molecular interaction between mycotoxins and bioreceptors, among which Abs and aptamers (Apts) are the most well‐accepted bioreceptors (Kaur et al., 2023). The properties of the bioreceptors are the decisive factor in determining whether the mycotoxin can be specifically “fished” from the complex matrices.…”

Section: Recognition Elements (Ab and Aptamer) In Preparing Mycotoxin...mentioning

confidence: 99%

Magnetic nanoparticle‐based immunosensors and aptasensors for mycotoxin detection in foodstuffs: An update

Gao,

Zhou,

Zhang

et al. 2023

Comp Rev Food Sci Food Safe

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Mycotoxin contamination of food crops is a global challenge due to their unpredictable occurrence and severe adverse health effects on humans. Therefore, it is of great importance to develop effective tools to prevent the accumulation of mycotoxins through the food chain. The use of magnetic nanoparticle (MNP)‐assisted biosensors for detecting mycotoxin in complex foodstuffs has garnered great interest due to the significantly enhanced sensitivity and accuracy. Within such a context, this review includes the fundamentals and recent advances (2020–2023) in the area of mycotoxin monitoring in food matrices using MNP‐based aptasensors and immunosensors. In this review, we start by providing a comprehensive introduction to the design of immunosensors (natural antibody or nanobody, random or site‐oriented immobilization) and aptasensors (techniques for aptamer selection, characterization, and truncation). Meanwhile, special attention is paid to the multifunctionalities of MNPs (recoverable adsorbent, versatile carrier, and signal indicator) in preparing mycotoxin‐specific biosensors. Further, the contribution of MNPs to the multiplexing determination of various mycotoxins is summarized. Finally, challenges and future perspectives for the practical applications of MNP‐assisted biosensors are also discussed. The progress and updates of MNP‐based biosensors shown in this review are expected to offer readers valuable insights about the design of MNP‐based tools for the effective detection of mycotoxins in practical applications.

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Recent Advances in Recognition Receptors for Electrochemical Biosensing of Mycotoxins—A Review

Cited by 3 publications

References 125 publications

Comprehensive Insights into Ochratoxin A: Occurrence, Analysis, and Control Strategies

Comprehensive Insights into Ochratoxin A: Occurrence, Analysis, and Control Strategies

Biosensors for Food Mycotoxin Determination: A Comparative and Critical Review

Magnetic nanoparticle‐based immunosensors and aptasensors for mycotoxin detection in foodstuffs: An update

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