Recent advances in quantum dots for biomedical applications

Lee, Jae Seo; Youn, Yun Hee; Kwon, Il Keun; Ko, Na Re

doi:10.1007/s40005-018-0387-3

Cited by 61 publications

(25 citation statements)

References 35 publications

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“…They are currently used in many applications, such as drug transport, DANN-sensing, bioimaging and tissue engineering. In addition, the use of these materials with drugs has produced positive results for their use with drug carriers, particular regarding their ability to reduce cell viability in diseases such as cancer (Zhang et al, 2017, Islam et al, 2017, Lee et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

Graphene quantum dots: Synthesis, characterization, cell viability, genotoxicity for biomedical applications

Şenel

Demir

Büyükköroğlu

et al. 2019

Saudi Pharmaceutical Journal

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We report the synthesis and applications of a novel N-doped graphene quantum dots (GQDs) using hydrothermal reaction between citric acid and p-aminophenol. The synthesized N-doped GQDs have been characterized physico-chemically and evaluated its antioxidant, antimicrobial, DNA binding and cleavage activities. siRNA loading studies were performed and their effects on cells were evaluated. Obtained results indicate that monodisperse solution of N-doped GQDs has been obtained with particles size ca. ∼10.9 ± 1.3 nm. UV–Vis spectroscopy studies of the interactions between the N-doped GQDs and calf thymus DNA (CT-DNA) showed that the compound interact with CT-DNA via both intercalative and electrostatic binding. The DNA cleavage study showed that the N-doped GQDs cleaved DNA without any external agents. The antioxidant activity of N-doped GQDS was very active when compared to BHT. As the concentration of the compound increased, the antioxidant activity also increased. Cell viability assay demonstrated that the Ndoped GQDs showed cell viability (70%) when the concentration reached 200 μg/mL for A549 and also MDA-MB-231, 150 μg/mL for NIH-3T3 cell lines at 24 h incubation. N-doped GQDs were coated with Eudragit RS 100 and EphA2-siRNA was loaded. As a result of the studies on these formulations, it was concluded that there may be significant effects on A549 cells. The microscopy results revealed that N-doped GQDs was quickly internalized into the cell. Our novel N-doped-GQDs with siRNA are candidate for in situ tumor suppression via DNA and mRNA breakage.

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Section: Resultsmentioning

confidence: 99%

Graphene quantum dots: Synthesis, characterization, cell viability, genotoxicity for biomedical applications

Şenel

Demir

Büyükköroğlu

et al. 2019

Saudi Pharmaceutical Journal

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“…Despite their versatility and large scope of possibilities for biosensing, one major disadvantage of QDs fabricated with inorganic materials is their toxicity, which limits their clinical applications. Graphene and other carbon-based QDs, unlike the conventional heavy-metals DQs, are biocompatible, environment friendly, and easier to prepare [7,8], and for that reason, they have gained considerable attention. Among the most recent examples of CQD-based is a fluorescent CQD biosensor for hydrogen peroxide detection and simultaneous monitoring of the acetylcholinesterase reaction system [7].…”

Section: Principles and Evolution Of Biosensing Techniquesmentioning

confidence: 99%

Dynamic Luminescent Biosensors Based on Peptides for Oxygen Determination

Souza¹,

Martins²,

Colmati³

2019

Biosensors for Environmental Monitoring

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This chapter summarizes the fundamentals of biosensing techniques based on fluorescence spectroscopy and the protagonism of state-of-the-art luminescent biosensors in a wide range of scientific areas, from environmental monitoring to diagnostics and decease treatment, focusing on the paramount contribution of biosensors based on the Förster Resonance Energy Transfer (FRET) transducing mechanism. State-of-the-art FRET biosensors are specially characterized by outstanding sensitivity toward a number of environmental pollutants and dissolved oxygen in aquatic ecosystems, capable of detecting concentrations in the nano and picomolar scales. These biosensors have also been showing impressive performance over other methods in the study of real-time biological processes in vivo relevant to help understanding decease progression like cancer.

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“…Nanoscale luminescent materials have attracted a special interest in numerous biological applications including biological tissue imaging, super-resolution imaging, sensitive optical sensing, optogenetic (brain studies), and drug delivery. These include organic dyes [ 1 , 2 , 3 ], quantum dots (QDs) [ 4 , 5 ], and fluorescent polymers [ 6 , 7 , 8 ]; however, they are limited by either photostability [ 9 ], toxicity [ 10 , 11 ], or chemical environment sensitivity [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Engineering Red-Enhanced and Biocompatible Upconversion Nanoparticles

et al. 2021

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The exceptional optical properties of lanthanide-doped upconversion nanoparticles (UCNPs) make them among the best fluorescent markers for many promising bioapplications. To fully utilize the unique advantages of the UCNPs for bioapplications, recent significant efforts have been put into improving the brightness of small UCNPs crystals by optimizing dopant concentrations and utilizing the addition of inert shells to avoid surface quenching effects. In this work, we engineered bright and small size upconversion nanoparticles in a core–shell–shell (CSS) structure. The emission of the synthesized CSS UCNPs is enhanced in the biological transparency window under biocompatible excitation wavelength by optimizing dopant ion concentrations. We also investigated the biosafety of the synthesized CSS UCNP particles in living cell models to ensure bright and non-toxic fluorescent probes for promising bioapplications.

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Recent advances in quantum dots for biomedical applications

Cited by 61 publications

References 35 publications

Graphene quantum dots: Synthesis, characterization, cell viability, genotoxicity for biomedical applications

Graphene quantum dots: Synthesis, characterization, cell viability, genotoxicity for biomedical applications

Dynamic Luminescent Biosensors Based on Peptides for Oxygen Determination

Engineering Red-Enhanced and Biocompatible Upconversion Nanoparticles

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