Recent advances in polyamine research

Kumar, Amar; Taylor, Mark A.; Altabella, Teresa; Tiburcio, Antonio F.

doi:10.1016/s1360-1385(97)01013-3

Cited by 356 publications

(225 citation statements)

References 41 publications

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“…Because of the pharmaceutical importance of polyaminederived alkaloids, biotechnological approaches aim to modify the accumulation and distribution of polyamines using overexpression and antisense inhibition of ADC, ODC, SPDS, SPMS, and SAMDC genes in various species (for review, see Kumar et al, 1997;Malmberg et al, 1998). These approaches revealed that the altered expression of polyamine biosynthesis genes often results in growth retardation, sterility, and other developmental defects.…”

Section: Discussionmentioning

confidence: 99%

“…Polyamines are abundant DNA and RNA binding organic cations that are implicated in the regulation of morphogenesis, stress responses, senescence, and other basic processes in higher plants (Tiburcio et al, 1990;Kumar et al, 1997;Malmberg et al, 1998). The diamine putrescine is synthesized by either Orn or Arg decarboxylase (ODC and ADC, respectively) and converted to spermidine and spermine through the sequential addition of aminopropyl residues by spermidine and spermine synthases (SPDS and SPMS, respectively) (Tabor and Tabor, 1984;Pegg, 1986;Cohen, 1998).…”

Section: Introductionmentioning

confidence: 99%

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A Polyamine Metabolon Involving Aminopropyl Transferase Complexes in Arabidopsis

Panicot

Minguet

Ferrando³

et al. 2002

Plant Cell

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162

126

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The conversion of putrescine to spermidine in the biosynthetic pathway of plant polyamines is catalyzed by two closely related spermidine synthases, SPDS1 and SPDS2, in Arabidopsis. In the yeast two-hybrid system, SPDS2 was found to interact with SPDS1 and a novel protein, SPMS (spermine synthase), which is homologous with SPDS2 and SPDS1. SPMS interacts with both SPDS1 and SPDS2 in yeast and in vitro. Unlike SPDS1 and SPDS2, SPMS failed to suppress the spe ⌬ 3 deficiency of spermidine synthase in yeast. However, SPMS was able to complement the spe ⌬ 4 spermine deficiency in yeast, indicating that SPMS is a novel spermine synthase. The SPDS and SPMS proteins showed no homodimerization but formed heterodimers in vitro. Pairwise coexpression of hemagglutinin-and c-Myc epitope-labeled proteins in Arabidopsis cells confirmed the existence of coimmunoprecipitating SPDS1-SPDS2 and SDPS2-SPMS heterodimers in vivo. The epitope-labeled SPDS and SPMS proteins copurified with protein complexes ranging in size from 650 to 750 kD. Our data demonstrate the existence of a metabolon involving at least the last two steps of polyamine biosynthesis in Arabidopsis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Polyamine Metabolon Involving Aminopropyl Transferase Complexes in Arabidopsis

Panicot

Minguet

Ferrando³

et al. 2002

Plant Cell

Self Cite

162

126

View full text Add to dashboard Cite

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“…4B, the SPDS2 transcript level was increased by treatment of seedlings with cytokinin (100 WM kinetin) for 2 h. Cytokinin is essential for plant cell division and interacts synergistically or antagonistically with auxin to control many aspects of plant growth [23]. Taken together with the proposed functions of plant polyamines such as the control of cell division, transcription of genes, organ development and fruit ripening [3,4,7], our results suggest that cytokinin and auxin exert their e¡ects at least in part by the production of spermidine and spermine. It has recently been reported that exogenous spermine prevents cytokinin-induced expression of ARR5, one of the response regulator genes in Arabidopsis [24].…”

Section: Expression Analysesmentioning

confidence: 98%

Characterization of the spermidine synthase‐related gene family in Arabidopsis thaliana

Hanzawa¹,

Imai²,

Michael³

et al. 2002

FEBS Letters

114

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The Arabidopsis genome contains four genes that encode proteins similar to both spermidine synthase and spermine synthase of other organisms. Our previous study revealed that one of these genes, designated ACAULIS5 (ACL5), encodes spermine synthase and that its null mutation results in a severe defect in the elongation of stem internodes. Here we report the characterization of the other three genes, designated SPDS1, SPDS2 and SPDS3. Our results showed that SPDS1 and SPDS2 possess spermidine synthase activity in yeast spermidine synthase-de¢cient mutants, but the enzyme activity of SPDS3 remained to be determined. RNA gel blot analysis revealed that all of these genes are expressed in all plant organs but show di¡erent responses to exogenous plant hormones, suggesting that they are involved in di¡erent aspects of growth by modulating the contents of polyamines in plant cells. ß

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“…A function for an upregulated SAMDC could be in polyamine synthesis, in which this enzyme catalyzes a ratelimiting step (Kumar et al, 1997). Polyamines, which play important roles in plant development, are known to increase under abiotic stress (Evans and Malmberg, 1989).…”

Section: The Time-specific Response Of Transcripts In Pokkalimentioning

confidence: 99%

Gene Expression Profiles during the Initial Phase of Salt Stress in Rice

et al. 2001

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Transcript regulation in response to high salinity was investigated for salt-tolerant rice (var Pokkali) with microarrays including 1728 cDNAs from libraries of salt-stressed roots. NaCl at 150 mM reduced photosynthesis to one tenth of the prestress value within minutes. Hybridizations of RNA to microarray slides probed for changes in transcripts from 15 min to 1 week after salt shock. Beginning 15 min after the shock, Pokkali showed upregulation of transcripts. Approximately 10% of the transcripts in Pokkali were significantly upregulated or downregulated within 1 hr of salt stress. The initial differences between control and stressed plants continued for hours but became less pronounced as the plants adapted over time. The interpretation of an adaptive process was supported by the similar analysis of salinity-sensitive rice (var IR29), in which the immediate response exhibited by Pokkali was delayed and later resulted in downregulation of transcription and death. The upregulated functions observed with Pokkali at different time points during stress adaptation changed over time. Increased protein synthesis and protein turnover were observed at early time points, followed by the induction of known stress-responsive transcripts within hours, and the induction of transcripts for defenserelated functions later. After 1 week, the nature of upregulated transcripts (e.g., aquaporins) indicated recovery.

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Recent advances in polyamine research

Cited by 356 publications

References 41 publications

A Polyamine Metabolon Involving Aminopropyl Transferase Complexes in Arabidopsis

A Polyamine Metabolon Involving Aminopropyl Transferase Complexes in Arabidopsis

Characterization of the spermidine synthase‐related gene family in Arabidopsis thaliana

Gene Expression Profiles during the Initial Phase of Salt Stress in Rice

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