Recent Advances in Plant–Insect Interactions

Maffei, Massimo E.

doi:10.3390/ijms241411338

Cited by 5 publications

(2 citation statements)

References 19 publications

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“…Host plant damage by phytophagous insects alters plant nutrient content, production of toxic secondary metabolites, the activities of defense proteins and enzymes, and upregulates the expression of various defense-associated genes ( Badenes-Pérez, 2022 ; Beran and Petschenka, 2022 ). The redistribution of certain nutrients and rapid synthesis of secondary metabolites in plants after pest infestation affects the feeding, growth, and development of pests, which in turn stimulates insect resistance ( Erb and Reymond, 2019 ; Barbero and Maffei, 2023 ). Levels of soluble sugars, free amino acids, and soluble proteins in bean leaves decreased with increasing population density and feeding time of F. occidentalis , and were lower than those in control levels ( Qian et al., 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Physiological responses and transcriptome analysis of Hemerocallis citrina Baroni exposed to Thrips palmi feeding stress

Sun,

Shen,

Chen

et al. 2024

Front. Plant Sci.

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Thrips are serious pests of Hemerocallis citrina Baroni (daylily), affecting crop yield and quality. To defend against pests, daylily has evolved a set of sophisticated defense mechanisms. In the present study, induction of systemic resistance in Hemerocallis citrina ‘Datong Huanghua’ by Thrips palmi feeding was investigated at both biochemical and molecular levels. The soluble sugar content of daylily leaves was significantly lower than that in control check (CK) at all time points of feeding by T. palmi, whereas the amino acid and free fatty acid contents started to be significantly lower than those in CK after 7 days. Secondary metabolites such as tannins, flavonoids, and total phenols, which are harmful to the growth and reproduction of T. palmi, were increased significantly. The activities of defense enzymes such as peroxidase (POD), phenylalanine ammonia lyase (PAL), and polyphenol oxidase (PPO) were significantly increased, and the degree of damage to plants was reduced. The significant increase in protease inhibitor (PI) activity may lead to disrupted digestion and slower growth in T. palmi. Using RNA sequencing, 1,894 differentially expressed genes (DEGs) were identified between control and treatment groups at five timepoints. DEGs were mainly enriched in secondary metabolite synthesis, jasmonic acid (JA), salicylic acid (SA), and other defense hormone signal transduction pathways, defense enzyme synthesis, MAPK signaling, cell wall thickening, carbohydrate metabolism, photosynthesis, and other insect resistance pathways. Subsequently, 698 DEGs were predicted to be transcription factors, including bHLH and WRKY members related to biotic stress. WGCNA identified 18 hub genes in four key modules (Purple, Midnight blue, Blue, and Red) including MYB-like DNA-binding domain (TRINITY_DN2391_c0_g1, TRINITY_DN3285_c0_g1), zinc-finger of the FCS-type, C2-C2 (TRINITY_DN21050_c0_g2), and NPR1 (TRINITY_DN13045_c0_g1, TRINITY_DN855_c0_g2). The results indicate that biosynthesis of secondary metabolites, phenylalanine metabolism, PIs, and defense hormones pathways are involved in the induced resistance to T. palmi in daylily.

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Section: Introductionmentioning

confidence: 99%

Physiological responses and transcriptome analysis of Hemerocallis citrina Baroni exposed to Thrips palmi feeding stress

Sun,

Shen,

Chen

et al. 2024

Front. Plant Sci.

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“…DNA barcoding system for taxonomic identification is based on the assumption that sequence conservation is normally much higher intraspecifically than interspecifically [6]. Plant-insect molecular interaction, including plant defense signal production, insect elicitor perception and effector secretion for efficient infection, remains a perennial issue [7]. Taking advantage of high-throughput sequencing platforms, many insect genome maps have been drawn [8,9].…”

Section: Introductionmentioning

confidence: 99%

A Fast, Simple and Low-cost DNA Extraction Protocol from Common Ants and Beetles for Multiple Molecular Applications

Jiaying,

Junxia,

et al. 2024

BIO

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The rapid development of molecular biology tools in insect systematics, invasion research, evolutionary ecology and biodiversity analysis has led to faster and greater progress in understanding insect behavior and biology. Efficient DNA extraction is the foremost step and serves as the vital foundation. Several rapid DNA extraction methods have been established, which are often time-consuming and labour-intensive. Here, a simple, fast, low-cost DNA extraction protocol for common insect samples was developed basing on 28 specimens of 16 insect species (7 ants, 9 bark and ambrosia beetles). The new protocol was shown to be feasible and highly efficient by comparison with commercial kit in terms of DNA yield, purity and PCR sensitivity. The concentration of DNA through the new rapid method was higher than that through commercial kit, whether in ant or beetle samples. A better quality of DNA extracted via kit was indicated by A<sub>260</sub>/A<sub>280</sub> mostly ranging from 1.80 to 2.00. There was little difference between DNA extracted from adult and nymphal insects. PCR sensitivity of extracted DNA using both protocols was comparable. For nested PCR, amplification after two rounds yielded a bright signal using template DNA through both methods. But for PCR using primers of LCO1490 and HCO2198, the success ratio was lower (85.18%). Through BLAST, these amplicons were matched to related data with high identity. By combining this protocol with variable analysis platforms such as common PCR, loop-mediated isothermal amplification, and high throughput sequencing, it could assist insect diagnostics, biological surveys and invasion researches.

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Phytochemical strategies for combating Spodoptera litura (Fab.): a review of botanicals and their metabolites

Vasantha-Srinivasan,

Han,

Karthi

et al. 2024

Toxin Reviews

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Recent Advances in Plant–Insect Interactions

Abstract: Plant–insect interaction is a fast-developing research field that continues to increase the interest of numerous scientists, many of whom come from heterogeneous backgrounds [...]

Cited by 5 publications

References 19 publications

Physiological responses and transcriptome analysis of Hemerocallis citrina Baroni exposed to Thrips palmi feeding stress

Physiological responses and transcriptome analysis of Hemerocallis citrina Baroni exposed to Thrips palmi feeding stress

A Fast, Simple and Low-cost DNA Extraction Protocol from Common Ants and Beetles for Multiple Molecular Applications

Phytochemical strategies for combating Spodoptera litura (Fab.): a review of botanicals and their metabolites

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