Recent Advances in Oxygen Measurements Using Phosphorescence Quenching

Wilson, David F.; Vinogradov, Sergei A.

doi:10.1007/978-1-4615-1875-4_8

Cited by 8 publications

(4 citation statements)

References 27 publications

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“…Similar to previously published investigations (13,14), decrease in the environment increases the phosphorescence intensity (I) and lengthens the phosphorescence decay () of PdTCPPNa 4 , and inversely. Here, I and  were followed, as markers of the PO 2 variations, in acellular solutions or in PdTCPPNa 4 -loaded endothelial cells.…”

Section: Methodssupporting

confidence: 90%

NO Synthesis, Unlike Respiration, Influences Intracellular Oxygen Tension

Coste

Vial

Faury

et al. 2002

Biochemical and Biophysical Research Communications

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International audienceWe have developed a new phosphorescent probe, PdTCPPNa 4 , whose luminescence properties are affected by local variations of intracellular oxygen tension (PO 2). Spectrofluorometric measurements on living human umbilical venous endothelial cells loaded with this molecule show that a decrease in extracellular oxygen tension induces a decrease of PO 2 , illustrating the phenomenon of oxygen diffusion and validating the use of this probe in living cells. Moreover, KCN-or 2,4-dinitrophenol-induced modifications of respiration do not lead to detectable PO 2 variations, probably because O 2 diffusion is sufficient to allow oxygen supply. On the contrary, activation by acetylcholine or endothelial nitric oxide synthase (eNOS), which produces NO while consuming oxygen, induces a significant decrease in PO 2 , whose amplitude is dependent on the acetylcho-line dose, i.e., the eNOS activity level. Hence, activated cytosolic enzymes could consume high levels of oxygen which cannot be supplied by diffusion, leading to PO 2 decrease. Other cell physiology mechanisms leading to PO 2 variations can now be studied in living cells with this probe

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Section: Methodssupporting

confidence: 90%

NO Synthesis, Unlike Respiration, Influences Intracellular Oxygen Tension

Coste

Vial

Faury

et al. 2002

Biochemical and Biophysical Research Communications

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“…Various methods are available to assess tissue oxygenation, ranging from noninvasive and indirect methods to invasive and direct methods [28][29][30][31]. In this study, a polarographic pO 2 needle electrode was used to obtain 100 intrahepatic pO 2 values according to the pilgrim step method [32].…”

Section: Discussionmentioning

confidence: 99%

Intrahepatic Tissue pO<sub>2</sub> during Continuous or Intermittent Vascular Inflow Occlusion in a Pig Liver Resection Model

et al. 1998

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Background: Temporary vascular inflow occlusion of the liver (clamping of the hepatic pedicle) can prevent massive blood loss during liver resections. In this study, intrahepatic tissue pO₂ was assessed as parameter of microcirculatory disturbances induced by ischemia and reperfusion (I/R) in the liver following continuous (Cnt) or intermittent (Int) clamping in a hemihepatectomy model in the pig. Methods: Pigs (20–34 kg) were divided into 2 groups: I/R without hemihepatectomy (–HH; n = 10) and I/R with hemihepatectomy (+HH; n = 8). Ischemia during 90 min was Cnt or Int (6 sequential periods of 12 min of ischemia and 3 min of reperfusion), followed by 120 min of reperfusion. Intrahepatic pO₂ histograms (polarographic pO₂ needle electrode) were constructed before ischemia, at the end of 90 min of ischemia and after 120 min of reperfusion, along with assessment of plasma AST, ALT and LDH. Bile production was monitored continuously. Results: Cumulative frequency distribution curves (CFDC) after 120 min of reperfusion in the Cnt–HH group were not different from preischemic CFDC (means ± SEM), whereas in the Int–HH group a left shift occurred indicating more hypo(non)perfused liver areas (pO₂ < 10 mm Hg: 2.6 ± 1.2 and 41.0 ± 17.5% in Cnt–HH and Int–HH; p < 0.01). In the Cnt+HH group, a left shift in the CFDC occurred. In the Int+HH group, a left and a right shift occurred simultaneously, indicating both hypo(non)- and hyperperfused (shunting) liver areas (pO₂ < 10 mm Hg: 4.0 ± 2.7 and 9.6 ± 8.5%, n.s., and pO₂ > 60 mm Hg: 2.0 ± 2.0 and 17.3 ± 6.4%, p = 0.015, in Cnt+HH and Int+HH). Plasma AST, ALT and LDH levels were not increased after 120 min of reperfusion, except for AST in Cnt+HH and Int+HH (from 54.6 ± 14.0 to 270.4 ± 42.8 U/l, p < 0.01, and from 47.8 ± 9.4 to 176.5 ± 55.9 U/l, n.s.). Bile production (percentage of mean preischemic value) during 120 min of reperfusion was significantly reduced in the Int–HH group, as compared to the Cnt–HH group (57.0 and 117.0% after 120 min of reperfusion, p = 0.002). In Cnt+HH and Int+HH, bile production was significantly reduced (33.3 ± 20.0%, p = 0.05, and 38.5 ± 7.9%, p = 0.007); however it was not different between the two groups. Conclusions: (1) Intrahepatic tissue pO₂ as indicator of microvascular perfusion is a parameter of early I/R injury; (2) continuous vascular inflow occlusion resulted in less microcirculatory disturbances, when compared to intermittent occlusion.

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“…However, the phosphorescence lifetime imaging method has proven very useful for window chamber studies. The basis for this measurement relies on phosphorescence that is emitted from a metal porphyrin complex after being illuminated with visible light [115][116][117][118][119]. The decay time for the phosphorescence is oxygen dependent, which forms the basis for the measurement [120].…”

Section: Tumor Oxygenation and Extracellular Phmentioning

confidence: 99%

Intravital Fluorescence Facilitates Measurement of Multiple Physiologic Functions and Gene Expression in Tumors of Live Animals

et al. 2002

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The purpose of this report is to present an overview of the use of fluorescence imaging in vivo, with particular emphasis on oncology. It is important to note, however, that many of the methods described herein have been applied to the study of non-malignant tissues as well. Modern medicine and biology research has benefited greatly from an ever-expanding assortment of fluorescent markers and labels. These markers and labels have allowed investigators to observe the behavior and properties of cell and molecular entities of interest in the context of complicated biological systems such as a mammalian cell or a whole mouse. Methods developed to image fluorescence in whole mice have been valuable in studying patterns of tumor growth and metastases. Alternatively, more detailed information and a wide variety of endpoints can be obtained using “intravital” preparations. This review focuses on use of fluorescence imaging for intravital preparations. For detail on fluorescence imaging of whole animals, refer to reviews on this subject [1,2]. For oncologic applications, studies have focused primarily on window chamber preparations that allow for real-time visualization of tumor growth, vascularity, vascular responses to stimulation, vascular permeability, vascular orientation, flow instability, and the like. These endpoints have been used to show that there are functional differences between tumor and normal tissues with respect to these functions under baseline conditions and after therapeutic manipulation. Examples of some of these differences are provided in this review as a means to illustrate how they can be used.

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Recent Advances in Oxygen Measurements Using Phosphorescence Quenching

Cited by 8 publications

References 27 publications

NO Synthesis, Unlike Respiration, Influences Intracellular Oxygen Tension

NO Synthesis, Unlike Respiration, Influences Intracellular Oxygen Tension

Intrahepatic Tissue pO<sub>2</sub> during Continuous or Intermittent Vascular Inflow Occlusion in a Pig Liver Resection Model

Intravital Fluorescence Facilitates Measurement of Multiple Physiologic Functions and Gene Expression in Tumors of Live Animals

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