Recent advances in nucleic acid-based methods for detection of helminth infections and the perspective of biosensors for future development

Ullah, Hanif; Qadeer, Abdul; Rashid, Muhammad Imran; Cheng, Guofeng

doi:10.1017/s0031182019001665

Cited by 14 publications

(8 citation statements)

References 119 publications

(130 reference statements)

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“…Moreover, diagnostic assays commonly used for detecting many STHs infections are considered invasive and risky as they involve collecting samples such as body fluids (e.g., stool and blood) and tissues through biopsy, requiring both special equipment and specialist, which is often a big challenge in developing countries. There has been continued effort toward developing more sensitive, low-cost, and easy-to-operate molecular diagnostics, which can be used in both endemic and lowendemicity settings, and this topic is reviewed elsewhere (Ullah et al, 2020;Khurana et al, 2021). Recent findings about the immunomodulatory roles of helminth-derived ESPs, including proteins, microRNAs, and extracellular vesicles (Hoy et al, 2014;Kammoun-Rebai et al, 2016;Bi et al, 2021), is a paradigm shift in diagnosis/prognosis of helminthiasis and more about proteins/protein microarrays, microRNAs, and extracellular vesicles are available in these reviews (Sotillo et al, 2020;Li et al, 2021;Mu et al, 2021).…”

Section: Potential Application Of Small Biomarker Molecules In Diagno...mentioning

confidence: 99%

Immunomodulatory and biological properties of helminth-derived small molecules: Potential applications in diagnostics and therapeutics

et al. 2022

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Parasitic helminths secrete and excrete a vast array of molecules known to help skew or suppress the host’s immune response, thereby establishing a niche for sustained parasite maintenance. Indeed, the immunomodulatory potency of helminths is attributed mainly to excretory/secretory products (ESPs). The ESPs of helminths and the identified small molecules (SM) are reported to have diverse biological and pharmacological properties. The available literature reports only limited metabolites, and the identity of many metabolites remains unknown due to limitations in the identification protocols and helminth-specific compound libraries. Many metabolites are known to be involved in host-parasite interactions and pathogenicity. For example, fatty acids (e.g., stearic acid) detected in the infective stages of helminths are known to have a role in host interaction through facilitating successful penetration and migration inside the host. Moreover, excreted/secreted SM detected in helminth species are found to possess various biological properties, including anti-inflammatory activities, suggesting their potential in developing immunomodulatory drugs. For example, helminths-derived somatic tissue extracts and whole crude ESPs showed anti-inflammatory properties by inhibiting the secretion of proinflammatory cytokines from human peripheral blood mononuclear cells and suppressing the pathology in chemically-induced experimental mice model of colitis. Unlike bigger molecules like proteins, SM are ideal candidates for drug development since they are small structures, malleable, and lack immunogenicity. Future studies should strive toward identifying unknown SM and isolating the under-explored niche of helminth metabolites using the latest metabolomics technologies and associated software, which hold potential keys for finding new diagnostics and novel therapeutics.

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Section: Potential Application Of Small Biomarker Molecules In Diagno...mentioning

confidence: 99%

Immunomodulatory and biological properties of helminth-derived small molecules: Potential applications in diagnostics and therapeutics

et al. 2022

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“…Biosensors are an influential and creative analytical tool with admirable classifications, very sensitive, quick, simple, and low-cost. It can be used in the field of drug finding, diagnosis, biomedicine, food safety, and environmental monitoring (Ullah et al, 2020c). As a result, these devices have become extensively established and employed in a variety of biological disciplines, including cancer detection, diagnostic reagent creation, and gene therapy.…”

Section: Advantages and Disadvantages Of Nanotechnologymentioning

confidence: 99%

“…The relatively high cost and adverse environmental impact of the most often utilized NPs, such as gold, iron oxide, or ZnO-like NPs, severely limit their agro-biotechnological uses (Sun et al, 2008;Tang et al, 2012). The biosensor is also not commercially available for the majority of infections; further research is needed to evolve the technology (Janissen et al, 2017;Ullah et al, 2020c).…”

Section: Advantages and Disadvantages Of Nanotechnologymentioning

confidence: 99%

Potential application of nanotechnology in the treatment, diagnosis, and prevention of schistosomiasis

Qadeer

Ullah²,

Sohail³

et al. 2022

Front. Bioeng. Biotechnol.

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Schistosomiasis is one of the neglected tropical diseases that affect millions of people worldwide. Globally, it affects economically poor countries, typically due to a lack of proper sanitation systems, and poor hygiene conditions. Currently, no vaccine is available against schistosomiasis, and the preferred treatment is chemotherapy with the use of praziquantel. It is a common anti-schistosomal drug used against all known species of Schistosoma. To date, current treatment primarily the drug praziquantel has not been effective in treating Schistosoma species in their early stages. The drug of choice offers low bioavailability, water solubility, and fast metabolism. Globally drug resistance has been documented due to overuse of praziquantel, Parasite mutations, poor treatment compliance, co-infection with other strains of parasites, and overall parasitic load. The existing diagnostic methods have very little acceptability and are not readily applied for quick diagnosis. This review aims to summarize the use of nanotechnology in the treatment, diagnosis, and prevention. It also explored safe and effective substitute approaches against parasitosis. At this stage, various nanomaterials are being used in drug delivery systems, diagnostic kits, and vaccine production. Nanotechnology is one of the modern and innovative methods to treat and diagnose several human diseases, particularly those caused by parasite infections. Herein we highlight the current advancement and application of nanotechnological approaches regarding the treatment, diagnosis, and prevention of schistosomiasis.

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“…This demonstrates their utility in both clinical and personalized medicine. However, it has several limitations, such as the high cost of the instrumentation required for thermal cycling and signal detection, and the need for well-equipped laboratories ( 7 ). miRNAs biomarkers has the potential to address the shortcomings of other diagnostic tests, including cross-corrections, false negatives, low sensitivity, and specificity ( 111 , 112 ).…”

Section: Limitation Of Circulatory Mirna Biomarkermentioning

confidence: 99%

“…The World Health Organization (WHO) has already devised a strategy for controlling and eliminating helminth-related neglected tropical diseases. The most important helminth infection control strategy is mass drug administration ( 7 ).…”

Section: Introductionmentioning

confidence: 99%

Circulatory microRNAs in helminthiases: Potent as diagnostics biomarker, its potential role and limitations

Ullah

Tian²,

Arbab³

et al. 2022

Front. Vet. Sci.

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Infections caused by helminths are responsible for severe public health problems and economic burden on continental scale. Well-timed and precise diagnosis of helminth infections is critical for taking by appropriate approaches for pathogen control. Circulating miRNAs are stable diagnostic tool for different diseases found in a variety of body fluid. As diagnostic biomarkers in infectious diseases, miRNAs detection in body fluids of helminth infected hosts is growing promptly. Uncovering miRNAs is a relatively new tool, used for early-stage detection of helminth infection from experimental or non-invasive clinical samples. miRNAs can be detected in body fluids such as serum, saliva, urine, and tissues of helminth infected host, mainly blood offering important benefits for diagnosis accurately. In this review, we discuss different characteristics of helminth parasite-derived circulating and EV miRNAs, supporting its potential uses in for helminth diagnosis and treatment efficiency.

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Recent advances in nucleic acid-based methods for detection of helminth infections and the perspective of biosensors for future development

Cited by 14 publications

References 119 publications

Immunomodulatory and biological properties of helminth-derived small molecules: Potential applications in diagnostics and therapeutics

Immunomodulatory and biological properties of helminth-derived small molecules: Potential applications in diagnostics and therapeutics

Potential application of nanotechnology in the treatment, diagnosis, and prevention of schistosomiasis

Circulatory microRNAs in helminthiases: Potent as diagnostics biomarker, its potential role and limitations

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