Recent Advances in Nanomaterials with Inherent Optical and Magnetic Properties for Bioimaging and Imaging-Guided Nucleic Acid Therapy

Li, Yujing; Wang, Xudong; Zhang, Yinlong; Nie, Guangjun

doi:10.1021/acs.bioconjchem.0c00126

Cited by 13 publications

(3 citation statements)

References 118 publications

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“…We therefore set out to identify fluorescence labeled analogs to perform FRET based proximity studies and track the localization of the dendrimers during transfection. Indeed, various labeling studies reported on siRNA − or transfection reagents ,− have been shown to provide useful insight into the transfection process. Considering that a variety of dendrimer core modifications were compatible with siRNA transfection if the dendrimer branches were kept constant, we designed our fluorescently labeled dendrimers by modifying the dendrimer core with hydrophobic fluorescent labels.…”

Section: Introductionmentioning

confidence: 99%

Fluorescent Peptide Dendrimers for siRNA Transfection: Tracking pH Responsive Aggregation, siRNA Binding, and Cell Penetration

et al. 2020

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Transfecting nucleic acids into various cells is a key procedure in biological research also envisioned for therapeutic applications. In our effort to obtain simple reagents that would be readily accessible from commercial building blocks, we recently reported peptide dendrimers as single component siRNA transfection reagents accessible in pure form by solid-phase peptide synthesis. Here, we extend our studies of these dendrimers by identifying analogs bearing a coumarin or BODIPY fluorescent label in their core and displaying comparable siRNA transfection efficiencies, pH dependent aggregation, siRNA binding, and secondary structures. Fluorescence resonance energy transfer (FRET) studies show that the dendrimers are tightly associated with siRNA within the formed nanoparticles at pH 7.4 but are released into solution at pH 5.0 and can participate in endosome escape by destabilizing the membrane at this pH value. Colocalization studies furthermore suggest that peptide dendrimers and siRNA remain tightly associated throughout the transfection process.

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Section: Introductionmentioning

confidence: 99%

Fluorescent Peptide Dendrimers for siRNA Transfection: Tracking pH Responsive Aggregation, siRNA Binding, and Cell Penetration

et al. 2020

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“…[158] With the merging of dual-function performance, nanomaterials with intrinsic optical and magnetic characteristics were employed for both fluorescence bioimaging and imaging-guided nucleic acid therapy. [159] Additionally, nanomaterials were adopted for reducing the background signals and increasing the cellular uptake efficiency. For example, Wang et al designed an aptamer-carboxyfluorescein/graphene oxide nanocomplex, achieving the molecular probing in living cells.…”

Section: Integrated Nucleic Acids For Imagingmentioning

confidence: 99%

Materialistic Interfaces with Nucleic Acids: Principles and Their Impact

Zhang

et al. 2022

Adv Funct Materials

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Mendelian genetics spark the century‐old revolution in genetic engineering. DNA structural engineering may well spark a similar revolution in chemistry. The first few man‐made DNA structures are purely DNA but increased interest in hybrid DNA with other molecules is regaining popularity. Nucleic acids can interact with non‐nucleic acid‐based materials, showing their materialistic flexibility and potential and the prospect of forming new future materials. This review provides a deep materialistic background based on the interaction of nucleic acids with these non‐nucleic acid‐based materials covering comprehensive topics. The non‐nucleic acid materials are broadly divided into biological materials, for example, small‐biomolecules, peptides, proteins, and lipids, and non‐biological materials consisting of metal ions, chemical molecules, low‐dimensional nanomaterials, and micro‐scale polymers. Of significance, combining the unique properties of nucleic acids with these non‐genetic materials can bring about surprising synergistic characteristics and drive attractive applications in bio‐imaging, chem/bio‐sensing, disease therapies, antimicrobial works, and even information storage.

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“…The incorporation of siRNA into polymer-or lipid-coated iron oxide NPs generates multifunctional theranostic platforms that are suitable for simultaneous imaging and gene therapy (Figure 8E). 145,146 Since iron oxide NPs are capable of remotely controlled targeting and heating via the application of an external magnetic field, targeted transfection and combination therapy can be achieved. 147 Metal-organic frameworks (MOFs), formed by sensitive coordination bonds between inorganic metal ions and organic ligands, are another interesting vector due to the high surface area, porosity, and biodegradability (Figure 8F).…”

Section: Llmentioning

confidence: 99%