Recent Advances in Nanomaterials for Gene Delivery—A Review

Riley, Michael K; Vermerris, Wilfred

doi:10.3390/nano7050094

Cited by 305 publications

(193 citation statements)

References 121 publications

(199 reference statements)

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“…(Gao, Kim, & Liu, 2007;Karimi et al, 2015). Considering the induction of high immune response and carcinogenicity risks after viral vector exposure, recent efforts have shifted toward nonviral methods (Nayak & Herzog, 2010;Ramamoorth & Narvekar, 2015;Riley & Vermerris, 2017). However, some of the nonviral approaches might have lower ability to transverse nuclear membrane, lower efficiency and poor transgene expression, and/or some biosafety issues (Ramamoorth & Narvekar, 2015;T.…”

Section: Anticancer and Other Drugs' Deliverymentioning

confidence: 99%

Biomedical applications of carbon nanomaterials: Drug and gene delivery potentials

Mohajeri

Behnam

Sahebkar

2018

Journal Cellular Physiology

209

100

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One of the major components in the development of nanomedicines is the choice of the right biomaterial, which notably determines the subsequent biological responses. The popularity of carbon nanomaterials (CNMs) has been on the rise due to their numerous applications in the fields of drug delivery, bioimaging, tissue engineering, and biosensing. Owing to their considerably high surface area, multifunctional surface chemistry, and excellent optical activity, novel functionalized CNMs possess efficient drug-loading capacity, biocompatibility, and lack of immunogenicity. Over the past few decades, several advances have been made on the functionalization of CNMs to minimize their health concerns and enhance their biosafety. Recent evidence has also implied that CNMs can be functionalized with bioactive peptides, proteins, nucleic acids, and drugs to achieve composites with remarkably low toxicity and high pharmaceutical efficiency. This review focuses on the three main classes of CNMs, including fullerenes, graphenes, and carbon nanotubes, and their recent biomedical applications.

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Section: Anticancer and Other Drugs' Deliverymentioning

confidence: 99%

Biomedical applications of carbon nanomaterials: Drug and gene delivery potentials

Mohajeri

Behnam

Sahebkar

2018

Journal Cellular Physiology

209

100

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“…Gene therapy affords a promising and efficient way to treat congenital and acquired diseases by delivering therapeutic genes into target cells to promote or rectify the expression of specific genes . In recent years, with the rapid development of nanotechnology, many DNA and RNA delivery systems such as non‐viral gene carriers have become available that can be used for gene therapy instead of viral carriers .…”

Section: The Remaining Percent Of the Fluorescence Intensity In Fluormentioning

confidence: 99%

Multifunctional Gene Carriers Labeled by Perylene Diimide Derivative as Fluorescent Probe for Tracking Gene Delivery

Hao

Guo

et al. 2019

Macromol. Rapid Commun.

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Multifunctional carriers with both gene transfection property and fluorescent tracking function have attracted significant attention in recent years. Herein, a kind of perylene diimide derivative (PDI‐C10C8) is conjugated onto the polyethylenimine‐g‐poly(lactide‐co‐glycolide)‐g‐polyethylenimine (PLGA‐PEI) polymer to obtain fluorescent multifunctional polymer and micelles (abbreviated as MP). Then, the REDV‐G‐TAT‐G‐NLS (TP‐G) peptide sequence is grafted onto this MP to obtain multifunctional micelles labeled by perylene diimide derivative (MP‐TP‐G). These micelles exhibit enhanced photobleaching stability compared with the reference Cy5‐labeled micelles, and the fluorescent images of cellular uptake show bright red emission without any background noise. Confocal laser scanning microscope (CLSM) experiments show that gene complexes can deliver gene into nucleus. MP‐TP‐G carriers do not enter into the cell nucleus, which proves that the nuclear localization signal sequence may not exert its nucleus accumulation ability via conjugating to the amphiphilic polymers. The high transfection efficiency and the enhanced photobleaching stability, combined with the ability to monitor the detailed process of cellular uptake and gene delivery, make these multifunctional micelles have great potential application for endothelialization of artificial blood vessels and gene delivery process study.

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“…The delivery of oligonucleotide or genetic material to specific cells has been a long‐term goal for treatment of intractable diseases such as cancer, cystic fibrosis, retinal disorders, and cardiovascular disease. A range of potential gene delivery systems, both viral and non‐viral, have been used for the delivery of pDNA, siRNA, mRNA, and miRNA . Viral gene delivery systems have high efficiencies and generally show good transfection properties in vitro and in vivo, with several in clinical trials, and one (Glybera) recently approved in the EU .…”

Section: Introductionmentioning

confidence: 99%

“…Despite the advantages of non‐viral gene delivery vectors, in the past they have been slow to progress to clinical use due to their generally lower efficiency of gene delivery. Recent understanding of the barriers to efficient non‐viral vector delivery, such as nanoparticle instability in vivo, poor targeting to specific cells, and inefficient transport through biological barriers such as the cell membrane, has led to an increased number of candidate vectors currently in clinical trials . However, further improvements in these areas are still needed to realise the potential of gene‐based therapies, in particular in the treatment of cancers, where approaches such as suicide gene therapy, regulation of gene expression by delivery of miRNA, p53 replacement gene therapy, and redirection of T‐cell specificity towards cancer cells have recently shown promise.…”

Section: Introductionmentioning

confidence: 99%

Development of lipopolyplexes for gene delivery: A comparison of the effects of differing modes of targeting peptide display on the structure and transfection activities of lipopolyplexes

Bofinger

Thin

Mitchell

et al. 2018

Journal of Peptide Science

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The design, synthesis and formulation of non‐viral gene delivery vectors is an area of renewed research interest. Amongst the most efficient non‐viral gene delivery systems are lipopolyplexes, in which cationic peptides are co‐formulated with plasmid DNA and lipids. One advantage of lipopolyplex vectors is that they have the potential to be targeted to specific cell types by attaching peptide targeting ligands on the surface, thus increasing both the transfection efficiency and selectivity for disease targets such as cancer cells. In this paper, we have investigated two different modes of displaying cell‐specific peptide targeting ligands at the surface of lipopolyplexes. Lipopolyplexes formulated with bimodal peptides, with both receptor binding and DNA condensing sequences, were compared with lipopolyplexes with the peptide targeting ligand directly conjugated to one of the lipids. Three EGFR targeting peptide sequences were studied, together with a range of lipid formulations and maleimide lipid structures. The biophysical properties of the lipopolyplexes and their transfection efficiencies in a basal‐like breast cancer cell line were investigated using plasmid DNA bearing genes for the expression of firefly luciferase and green fluorescent protein. Fluorescence quenching experiments were also used to probe the macromolecular organisation of the peptide and pDNA components of the lipopolyplexes. We demonstrated that both approaches to lipopolyplex targeting give reasonable transfection efficiencies, and the transfection efficiency of each lipopolyplex formulation is highly dependent on the sequence of the targeting peptide. To achieve maximum therapeutic efficiency, different peptide targeting sequences and lipopolyplex architectures should be investigated for each target cell type.

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Recent Advances in Nanomaterials for Gene Delivery—A Review

Cited by 305 publications

References 121 publications

Biomedical applications of carbon nanomaterials: Drug and gene delivery potentials

Biomedical applications of carbon nanomaterials: Drug and gene delivery potentials

Multifunctional Gene Carriers Labeled by Perylene Diimide Derivative as Fluorescent Probe for Tracking Gene Delivery

Development of lipopolyplexes for gene delivery: A comparison of the effects of differing modes of targeting peptide display on the structure and transfection activities of lipopolyplexes

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