Recent advances in miniaturized microfluidic flow cytometry for clinical use

Chung, Taek Dong; Kim, Hee Chan

doi:10.1002/elps.200700620

Cited by 121 publications

(112 citation statements)

References 77 publications

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“…For other nonfluorescence-activated cell sorting mechanisms, such as magnetophoresis 77 and acoustophoresis, 78 readers can refer to a number of recently published articles. 12,[79][80][81] …”

Section: Microfluidic Fluorescence-activated Cell Sorter " Facs…mentioning

confidence: 99%

Review Article: Recent advancements in optofluidic flow cytometer

Cho¹,

Godin²,

Chen³

et al. 2010

Biomicrofluidics

141

105

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There is an increasing need to develop optofluidic flow cytometers. Optofluidics, where optics and microfluidics work together to create novel functionalities on a small chip, holds great promise for lab-on-a-chip flow cytometry. The development of a low-cost, compact, handheld flow cytometer and microfluorescence-activated cell sorter system could have a significant impact on the field of point-of-care diagnostics, improving health care in, for example, underserved areas of Africa and Asia, that struggle with epidemics such as HIV/AIDS. In this paper, we review recent advancements in microfluidics, on-chip optics, novel detection architectures, and integrated sorting mechanisms.

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Section: Microfluidic Fluorescence-activated Cell Sorter " Facs…mentioning

confidence: 99%

Review Article: Recent advancements in optofluidic flow cytometer

Cho¹,

Godin²,

Chen³

et al. 2010

Biomicrofluidics

141

105

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“…Indeed, the microfluidic approach favors analysis at the single-cell level, a reduced sample contamination, an increased analysis sensitivity and a higher processing automation 1,2 . To be truly competitive with standard instrumentation, these microfluidic devices should satisfy several challenging constraints: (i) a high throughput, necessary to process large amounts of samples in a reasonable amount of time; (ii) a low pressure drop over the microfluidic device, which basically means low fluidic resistance, thus allowing vacuum driven flows; (iii) optical accessibility inside the microchannel to allow high quality optical detection 3 .…”

Section: Introductionmentioning

confidence: 99%

Particle focusing by 3D inertial microfluidics

et al. 2017

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Three-dimensional (3D) particle focusing in microfluidics is a fundamental capability with a wide range of applications, such as on-chip flow cytometry, where high-throughput analysis at the single-cell level is performed. Currently, 3D focusing is achieved mainly in devices with complex layouts, additional sheath fluids, and complex pumping systems. In this work, we present a compact microfluidic device capable of 3D particle focusing at high flow rates and with a small footprint, without the requirement of external fields or lateral sheath flows, but using only a single-inlet, single-outlet microfluidic sequence of straight channels and tightly curving vertical loops. This device exploits inertial fluidic effects that occur in a laminar regime at sufficiently high flow rates, manipulating the particle positions by the combination of inertial lift forces and Dean drag forces. The device is fabricated by femtosecond laser irradiation followed by chemical etching, which is a simple two-step process enabling the creation of 3D microfluidic networks in fused silica glass substrates. The use of tightly curving three-dimensional microfluidic loops produces strong Dean drag forces along the whole loop but also induces an asymmetric Dean flow decay in the subsequent straight channel, thus producing rapid cross-sectional mixing flows that assist with 3D particle focusing. The use of out-of-plane loops favors a compact parallelization of multiple focusing channels, allowing one to process large amounts of samples. In addition, the low fluidic resistance of the channel network is compatible with vacuum driven flows. The resulting device is quite interesting for high-throughput on-chip flow cytometry.

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“…In this application, increased analysis throughput is a secondary concern compared to increased sensitivity and specificity. Portable, miniaturized cytometry devices may also be in high demand during outbreaks of influenza for virus typing (9-12), and are currently available for HIV, tuberculosis, or malaria screening, complete blood cell count measurements, and cell screening assays (13,14). Increased throughput will also benefit cell screening for drug discovery (15).…”

mentioning

confidence: 99%

“…Conventional flow cytometry systems are not portable and require skilled operators. Microfluidic FACS systems have also been developed (25), incorporating on-chip optical elements including waveguides (13,26). Recently developed microfluidic optical systems have also used a DVD pickup head to detect beads and erythrocytes (27).…”

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confidence: 99%

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Microfluidic impedance‐based flow cytometry

Berardino²,

et al. 2010

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Microfabricated flow cytometers can detect, count, and analyze cells or particles using microfluidics and electronics to give impedance-based characterization. Such systems are being developed to provide simple, low-cost, label-free, and portable solutions for cell analysis. Recent work using microfabricated systems has demonstrated the capability to analyze micro-organisms, erythrocytes, leukocytes, and animal and human cell lines. Multifrequency impedance measurements can give multiparametric, high-content data that can be used to distinguish cell types. New combinations of microfluidic sample handling design and microscale flow phenomena have been used to focus and position cells within the channel for improved sensitivity. Robust designs will enable focusing at high flowrates while reducing requirements for control over multiple sample and sheath flows. Although microfluidic impedance-based flow cytometers have not yet or may never reach the extremely high throughput of conventional flow cytometers, the advantages of portability, simplicity, and ability to analyze single cells in small populations are, nevertheless, where chip-based cytometry can make a large impact. ' 2010International Society for Advancement of Cytometry Key terms microfluidics; impedance characterization; label free; single cell analysis; hydrodynamic focusing; sorting MICROFLUIDIC flow cytometers can bring many advantages to the field of flow cytometry (FCM). Compared to typical flow cytometry channel sizes, the miniaturized dimensions permit microfluidic systems to analyze single cells, to identify cellular variability in gene expression, or drug response within a cell population. Chipbased cytometers can have lower size and costs than conventional benchtop instruments, and may be portable. Today, the developmental aim for microfluidic systems is to reach the same sensitivity and capability for multiparametric analyses as delivered by conventional flow cytometers. Many efforts have been made to improve existing devices and to create new miniaturized high-end instruments. Microfluidic chips can incorporate on-chip cell preparation, cell culture, lysis, and modules for optical, electrophoretic, or genomic analysis (1). They are also suitable for analysis of cells in suspension as well as adherent cells.Miniaturized cytometry devices will have high impact in the development of point-of-care devices in developing countries. Accurate CD4 1 T-cell counts are used to monitor human immunodeficiency virus (HIV)-infected patients, and various thresholds of the number of CD4 1 T lymphocytes per ll of whole blood are used to start antiretroviral therapy (2-5). A simple, single-purpose CD4 cell counting device, which does not require standard laboratory equipment or trained laboratory personnel, could help some of the 33 million HIV-infected people worldwide monitor the stage of infection (6)(7)(8). In this application, increased analysis throughput is a secondary concern compared to increased sensitivity and specificity. Portable, miniatu...

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Recent advances in miniaturized microfluidic flow cytometry for clinical use

Cited by 121 publications

References 77 publications

Review Article: Recent advancements in optofluidic flow cytometer

Review Article: Recent advancements in optofluidic flow cytometer

Particle focusing by 3D inertial microfluidics

Microfluidic impedance‐based flow cytometry

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