Recent advances in microfluidics for single-cell functional proteomics

Gebreyesus, Sofani Tafesse; Muneer, Gul; Huang, Chih-Cheng; Siyal, Asad Ali; Anand, Mihir; Chen, Yu‐Ju; Tu, Hsiung‐Lin

doi:10.1039/d2lc01096h

Cited by 2 publications

(1 citation statement)

References 272 publications

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“…This is only one application out of several that require efficient pumping for quick PoC testing by leveraging cost-effective passive microfluidics technology, eliminating the need for external carrier fluids or cumbersome pumps [40]. An efficient capillary pump offers a versatile platform for a wide range of diagnostic possibilities, including optical, electrical, biological, and antibody-based assays, enabling the comprehensive measurement of various blood and health parameters including cells at the PoC [41,42].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Microfluidics for Point-of-Care Blood Sensing

Tavakolidakhrabadi,

Stark,

Bacher

et al. 2024

Biosensors

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Blood tests are widely used in modern medicine to diagnose certain illnesses and evaluate the overall health of a patient. To enable testing in resource-limited areas, there has been increasing interest in point-of-care (PoC) testing devices. To process blood samples, liquid mixing with active pumps is usually required, making PoC blood testing expensive and bulky. We explored the possibility of processing approximately 2 μL of whole blood for image flow cytometry using capillary structures that allowed test times of a few minutes without active pumps. Capillary pump structures with five different pillar shapes were simulated using Ansys Fluent to determine which resulted in the fastest whole blood uptake. The simulation results showed a strong influence of the capillary pump pillar shape on the chip filling time. Long and thin structures with a high aspect ratio exhibited faster filling times. Microfluidic chips using the simulated pump design with the most efficient blood uptake were fabricated with polydimethylsiloxane (PDMS) and polyethylene oxide (PEO). The chip filling times were tested with 2 μL of both water and whole blood, resulting in uptake times of 24 s for water and 111 s for blood. The simulated blood plasma results deviated from the experimental filling times by about 35% without accounting for any cell-induced effects. By comparing the flow speed induced by different pump pillar geometries, this study offers insights for the design and optimization of passive microfluidic devices for inhomogenous liquids such as whole blood in sensing applications.

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Section: Introductionmentioning

confidence: 99%