Recent advances in microextraction by packed sorbent for bioanalysis

Abdel‐Rehim, Mohamed

doi:10.1016/j.chroma.2009.09.053

Cited by 267 publications

(122 citation statements)

References 29 publications

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“…The usage of less amounts or no solvent, increasing sensitivity of analysis and user-friendly systems, should be pointed out. In this context, the microextraction by packed sorbent (MEPS) has emerged as an attractive alternative for sample preparation due to its simplicity, little solvent consumption (as small as 10 L), the need of very small sample volumes, and can be easily interfaced to LC-MS [49] and GC-MS to provide a completely automated MEPS/LC-MS or MEPS/GC-MS system [50][51][52]. The commercially available MEPS uses the same sorbents as conventional SPE columns and so is suitable for use with most existing methods by scaling the reagent and sample volumes.…”

Section: Introductionmentioning

confidence: 99%

A sensitive microextraction by packed sorbent-based methodology combined with ultra-high pressure liquid chromatography as a powerful technique for analysis of biologically active flavonols in wines

Silva

Gonçalves

Câmara

2012

Analytica Chimica Acta

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Section: Introductionmentioning

confidence: 99%

A sensitive microextraction by packed sorbent-based methodology combined with ultra-high pressure liquid chromatography as a powerful technique for analysis of biologically active flavonols in wines

Silva

Gonçalves

Câmara

2012

Analytica Chimica Acta

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“…Among many advantages, usage of little or no solvent, increasing sensitivity of analysis, and user-friendly system, should be pointed out. Another miniaturized technique is the microextraction by packed sorbent (MEPS), developed from the conventional SPE packed bed devices from mL bed volumes to mL volumes [29,30]. Briefly, when the sample passed through the solid support, the analytes are adsorbed to the solid phase packed in a barrel insert and needle (BIN) [24,25].…”

mentioning

confidence: 99%

New method for determination of (E)‐resveratrol in wine based on microextraction using packed sorbent and ultra‐performance liquid chromatography

Gonçalves

Câmara

2011

J of Separation Science

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An ultra-fast and improved analytical methodology based on microextraction by packed sorbent (MEPS) combined with ultra-performance LC (UPLC) was developed and validated for determination of (E)-resveratrol in wines. Important factors affecting the performance of MEPS such as the type of sorbent material (C2, C8, C18, SIL, and M1), number of extraction cycles, and sample volume were studied. The optimal conditions of MEPS extraction were obtained using C8 sorbent and small sample volumes (50-250 mL) in one extraction cycle (extract-discard) and in a short time period (about 3 min for the entire sample preparation step). (E)-Resveratrol was eluted by 1 Â 250 mL of the mixture containing 95% methanol and 5% water, and the separation was carried out on a highstrength silica HSS T3 analytical column (100 mm Â 2.1 mm, 1.8 mm particle size) using a binary mobile phase composed of aqueous 0.1% formic acid (eluent A) and methanol (eluent B) in the gradient elution mode (10 min of total analysis). The method was fully validated in terms of linearity, detection (LOD) and quantification (LOQ) limits, extraction yield, accuracy, and inter/intra-day precision, using a Madeira wine sample (ET) spiked with (E)-resveratrol at concentration levels ranging from 5 to 60 mg/mL. Validation experiments revealed very good recovery rate of 9575.8% RSD, good linearity with r 2 values 40.999 within the established concentration range, excellent repeatability (0.52%), and reproducibility (1.67%) values (expressed as RSD), thus demonstrating the robustness and accuracy of the MEPS C8 /UPLC-photodiode array (PDA) method. The LOD of the method was 0.21 mg/mL, whereas the LOQ was 0.68 mg/mL. The validated methodology was applied to 30 commercial wines (24 red wines and six white wines) from different grape varieties, vintages, and regions. On the basis of the analytical validation, the MEPS C8 /UPLC-PDA methodology shows to be an improved, sensitive, and ultra-fast approach for determination of (E)-resveratrol in wines with high resolving power within 6 min.

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“…Also, the MEPS technique is well designed to be directly coupled with liquid or gas chromatographic systems. The direct elution of sample components to the GC system is possible but usually requires a large volume injector to handle the solvent volume in the range of 10 to 100 μL [16]. Thus, MEPS extraction-fractionation technique may be a useful alternative for the classical separation procedures in a routine fractionation of cuticular wax extracts of plant origin.…”

Section: Analysis Of Plant Extractsmentioning

confidence: 99%

“…1 mL of organic solvents. The most frequent uses of the MEPS technique cover the analyses of drugs and their metabolites in biological samples [16][17][18] but also the analyses of fresh water contamination [19,20].…”

Section: Introductionmentioning

confidence: 99%

“…Several new extraction-separation techniques suitable for the use with low amounts of the sample were designed recently. Among them, microextraction by packed sorbent (MEPS™; SGE Analytical Science, Australia), which is a small-scale format of a classic solid-phase extraction, offers both polar and hydrophobic sorbents and is well suited to be used with chromatographic systems [16][17][18]. Reusable sorbent bed is integrated into a syringe, which may be used either manually or automatically to process the small volume (10 μL or more) of the liquid sample.…”

Section: Introductionmentioning

confidence: 99%

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Fractionation of Cuticular Waxes from the Leaves of Solanaceae Plant Species Using Microextraction by Packed Sorbent

Haliński

Stepnowski

2015

Acta Chromatographica

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Summary.The microextraction by packed sorbent (MEPS) was applied to a fractionation of cuticular wax extracts from several solanaceous plant species. A procedure developed requires only 0.6 mL of organic solvents and may be completed in less than 10 min. Hydrocarbons, which are frequently used in chemotaxonomy of Solanaceae, were almost exclusively eluted in one fraction. The amounts of most commonly detected polar wax components (alcohols, sterols, triterpenes) in the same fraction were reduced to ca. 34-46% of the total amounts in the whole extract. Despite the contamination of the hydrocarbon fraction with other wax components, the results obtained using MEPS and standard column chromatography on silica gel were similar when compared using cluster analysis based on the hydrocarbon profiles. However, the method was far less successful in removing the sucrose esters from extracts of Nicotiana rustica leaves. Thus, MEPS fractionation of plant cuticular waxes may be a fast and reliable alternative for the standard liquid chromatography techniques as long as no sucrose esters are present in the extract.

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Recent advances in microextraction by packed sorbent for bioanalysis

Cited by 267 publications

References 29 publications

A sensitive microextraction by packed sorbent-based methodology combined with ultra-high pressure liquid chromatography as a powerful technique for analysis of biologically active flavonols in wines

A sensitive microextraction by packed sorbent-based methodology combined with ultra-high pressure liquid chromatography as a powerful technique for analysis of biologically active flavonols in wines

New method for determination of (E)‐resveratrol in wine based on microextraction using packed sorbent and ultra‐performance liquid chromatography

Fractionation of Cuticular Waxes from the Leaves of Solanaceae Plant Species Using Microextraction by Packed Sorbent

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