Recent Advances in linear and nonlinear Raman spectroscopy I

Kiefer, W.

doi:10.1002/jrs.1902

Cited by 137 publications

(80 citation statements)

References 303 publications

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“…Furthermore, the detail in Raman spectra provides information on the molecular properties and composition of the sample which can be used for identification and quality control purposes. 7,8 When combined with chemometric data analysis, Raman spectroscopy also enables accurate quantitative assessment of the chemical composition of complex mixtures. The continual development of instrumentation and chemometric tools, has advanced the analysis of increasingly complex samples in many different application areas.…”

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confidence: 99%

A stainless steel multi‐well plate (SS‐MWP) for high‐throughput Raman analysis of dilute solutions

Ryder¹,

Vincentis²,

Ryan³

et al. 2010

J Raman Spectroscopy

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The use of Raman spectroscopy for the qualitative and quantitative analysis of dilute aqueous solutions is of interest to the biopharmaceutical manufacturing sector. However, the inherent weakness of the Raman effect, coupled with spectral variability due to spurious signals from sample holders, can produce significant problems for chemometric‐based high‐throughput assays. Therefore, there is a need for a multi‐well sample holder that ensures robust and repeatable measurements, in particular from dilute aqueous solutions such as cell culture media. Here we demonstrate the efficacy of a novel, electropolished, stainless steel multi‐well plate (SS‐MWP) sample holder with 96 wells for dilute aqueous solution analysis. A comprehensive study of the spectroscopic behaviour was carried out and comparisons made with multi‐well plates fabricated from polystyrene, polypropylene, and aluminium. A key factor in the validation studies is the use of intrinsically weak Raman scattering systems, e.g. water and dilute glucose solutions. The data collected show that the SS‐MWPs are much superior in terms of robustness, resistance to chemical attack, and measurement reproducibility and as such are the ideal sample holders for Raman analysis of dilute solutions. Copyright © 2010 John Wiley & Sons, Ltd.

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mentioning

confidence: 99%

A stainless steel multi‐well plate (SS‐MWP) for high‐throughput Raman analysis of dilute solutions

Ryder¹,

Vincentis²,

Ryan³

et al. 2010

J Raman Spectroscopy

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“…The 695 cm −1 band also characterizes the C2'-endo/anti conformation of B-DNA in aqueous solution (Kiefer, 2007). In Magnesium ion -DNA spectrum the 695 cm −1 band of metal free DNA shifts in the order 695→693→690→687→688 cm −1 at all the concentrations studied.…”

Section: Resultsmentioning

confidence: 88%

Structural Analysis of DNA Interactions with Magnesium Ion Studied by Raman Spectroscopy

M.¹

2011

American Journal of Biochemistry and Biotechnology

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Problem statement:In the present study, FT Raman spectroscopy had been used to extend our knowledge about Magnesium ion -DNA interactions at various volume ratios (1:50, 1:20, 1:10 and 1:5). Approach: The analysis of FT Raman data supported the existence of structural specificities in the interaction and also the stability of DNA secondary structure. Results: Results from the Raman spectra clearly indicate that the interaction of Magnesium ion with DNA is mainly through the phosphate groups of DNA with negligible change of the B-conformation of DNA at all the volume ratios studied. For example, band at 1079 cm −1 is assigned to the symmetrical stretching vibration of the nucleic acid phosphodioxy (PO-2) group. This band in the order 1079→ 1075→ 1070→ 1066→ 1063 cmat all Magnesium ion DNA concentrations studied. Similarly, Raman band at 845 cm −1 due to antisymmetrical phosphodiester (O-P-O) stretching of DNA. Conclusion: Magnesium ion interaction with the DNA phosphate is weak in comparison to interactions with the bases. On the other hand, the Raman signature of B-DNA is largely unperturbed by magnesium ion, suggesting much weaker interactions.

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“…The denominator ofρ (3) ac gives the saturation of the coherenceρ (3) bc when the laser fields become arbitrarily large. For strong field the spectrum may be affected by the focusing of the laser fields through the Ω 1,2 (r) = ℘ · E 1,2 (r)/ in the denominator.…”

Section: Integral Form Of the Scattered Fieldmentioning

confidence: 99%

“…The CARS process [1,2] has been used for developing a versatile real-time detection technique in spectroscopy and microscopy [3]. In particularly, backscattered ultra-violet CARS incorporated on LIDAR system [4] is promising for remote detection of molecular species present in hazardous biological aerosols [5,6].…”

Section: Introductionmentioning

confidence: 99%

Near-Field and Particle Size Effects in Coherent Raman Scattering

Ooi¹

2011

PIER

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Abstract-Nonlinear optical processes have been used for sensitive detection of chemicals, optical imaging and spectral analysis of small particles. We have developed an exact theoretical framework to study the angular dependence of coherent anti-Stokes Raman scattering (CARS) intensity in the near field and far field for nanoparticle and microparticle. We obtain exact analytical solution for the CARS signal valid for arbitrary detection distance. Interesting angular dependence is found for nanoparticle, especially with near field detection. The study includes the effects of focused lasers and particle size on the CARS intensity distribution. We find that the detection distance and particle size do not affect the spectroscopic peaks of CARS. However, interference of reflected waves in nanoparticle can produce a dip in the backscattered spectrum.

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Recent Advances in linear and nonlinear Raman spectroscopy I

Cited by 137 publications

References 303 publications

A stainless steel multi‐well plate (SS‐MWP) for high‐throughput Raman analysis of dilute solutions

A stainless steel multi‐well plate (SS‐MWP) for high‐throughput Raman analysis of dilute solutions

Structural Analysis of DNA Interactions with Magnesium Ion Studied by Raman Spectroscopy

Near-Field and Particle Size Effects in Coherent Raman Scattering

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