Recent Advances in Intermediate Filaments—Volume 1

Saéz, Ángela; González-Granado, José-María

doi:10.3390/ijms23105308

Cited by 5 publications

(2 citation statements)

References 19 publications

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“…S100β results indicate not only increases in the immunoreactivity of nuclear and cytoplasmic compartments suggesting downstream events associated with cascades (Davis and Syapin, 2004;Donato et al, 2009) but also a sort of protein relocalization that seems to be associated with the response to injury as previously described (Díaz-Amarilla et al, 2011;Jiménez-Riani et al, 2017). Such re-localization between different cellular compartments was previously seen in cytoplasmic or membrane proteins found as part of the nucleoskeleton (Philimonenko et al, 2004;Pellegrini and Budman, 2005;McCrea and Gottardi, 2016;Saez and Gonzalez-Granado, 2022) or as nuclear transcriptional coactivators or corepressors (Philimonenko et al, 2004;Pellegrini and Budman, 2005;Hobbs et al, 2016;McCrea and Gottardi, 2016).…”

Section: Effects Of Etoh And/or Cts On the Astrocyte Status And Membr...supporting

confidence: 73%

Astrocyte DNA damage and response upon acute exposure to ethanol and corticosterone

Reyes-Ábalos,

Álvarez-Zabaleta,

Olivera-Bravo

et al. 2024

Front. Toxicol.

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Introduction: Astrocytes are the glial cells responsible for brain homeostasis, but if injured, they could damage neural cells even deadly. Genetic damage, DNA damage response (DDR), and its downstream cascades are dramatic events poorly studied in astrocytes.Hypothesis and methods: We propose that 1 h of 400 mmol/L ethanol and/or 1 μmol/L corticosterone exposure of cultured hippocampal astrocytes damages DNA, activating the DDR and eliciting functional changes. Immunolabeling against γH2AX (chromatin DNA damage sites), cyclin D1 (cell cycle control), nuclear (base excision repair, BER), and cytoplasmic (anti-inflammatory functions) APE1, ribosomal nucleolus proteins together with GFAP and S100β plus scanning electron microscopy studies of the astrocyte surface were carried out.Results: Data obtained indicate significant DNA damage, immediate cell cycle arrest, and BER activation. Changes in the cytoplasmic signals of cyclin D1 and APE1, nucleolus number, and membrane-attached vesicles strongly suggest a reactivity like astrocyte response without significant morphological changes.Discussion: Obtained results uncover astrocyte genome immediate vulnerability and DDR activation, plus a functional response that might in part, be signaled through extracellular vesicles, evidencing the complex influence that astrocytes may have on the CNS even upon short-term aggressions.

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Section: Effects Of Etoh And/or Cts On the Astrocyte Status And Membr...supporting

confidence: 73%

Astrocyte DNA damage and response upon acute exposure to ethanol and corticosterone

Reyes-Ábalos,

Álvarez-Zabaleta,

Olivera-Bravo

et al. 2024

Front. Toxicol.

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“…Intermediate filaments (IFs) are a large family of structural polypeptides which are expressed only in metazoans. In humans, there are about 70 different IF genes that are divided into 6 types (type I to VI) based on physiochemical characteristics, sequence homology and tissue-specific expression [ 1 ]. Keratins are heteropolymers of type I and type II IFs and are expressed primarily in epithelia [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis Reveals Vimentin-Induced Disruption of Cell–Cell Associations Augments Breast Cancer Cell Migration

Usman

Jamal

Bushaala

et al. 2022

Cells

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In advanced metastatic cancers with reduced patient survival and poor prognosis, expression of vimentin, a type III intermediate filament protein is frequently observed. Vimentin appears to suppress epithelial characteristics and augments cell migration but the molecular basis for these changes is not well understood. Here, we have ectopically expressed vimentin in MCF-7 and investigated its genomic and functional implications. Vimentin changed the cell shape by decreasing major axis, major axis angle and increased cell migration, without affecting proliferation. Vimentin downregulated major keratin genes KRT8, KRT18 and KRT19. Transcriptome-coupled GO and KEGG analyses revealed that vimentin-affected genes were linked to either cell–cell/cell-ECM or cell cycle/proliferation specific pathways. Using shRNA mediated knockdown of vimentin in two cell types; MCF-7FV (ectopically expressing) and MDA-MB-231 (endogenously expressing), we identified a vimentin-specific signature consisting of 13 protein encoding genes (CDH5, AXL, PTPRM, TGFBI, CDH10, NES, E2F1, FOXM1, CDC45, FSD1, BCL2, KIF26A and WISP2) and two long non-coding RNAs, LINC00052 and C15ORF9-AS1. CDH5, an endothelial cadherin, which mediates cell–cell junctions, was the most downregulated protein encoding gene. Interestingly, downregulation of CDH5 by shRNA significantly increased cell migration confirming our RNA-Seq data. Furthermore, presence of vimentin altered the lamin expression in MCF-7. Collectively, we demonstrate, for the first time, that vimentin in breast cancer cells could change nuclear architecture by affecting lamin expression, which downregulates genes maintaining cell–cell junctions resulting in increased cell migration.

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Role of mechanotransduction in stem cells and cancer progression

Dey,

Adhikary,

Gopan

et al. 2024

Cancer Stem Cells and Signaling Pathways

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Recent Advances in Intermediate Filaments—Volume 1

Abstract: We would like to make readers of the second edition of the Special Issue from the International Journal of Molecular Sciences on the Recent Advances in Intermediate Filaments aware of the content of the first edition on this same topic [...]

Cited by 5 publications

References 19 publications

Astrocyte DNA damage and response upon acute exposure to ethanol and corticosterone

Astrocyte DNA damage and response upon acute exposure to ethanol and corticosterone

Transcriptome Analysis Reveals Vimentin-Induced Disruption of Cell–Cell Associations Augments Breast Cancer Cell Migration

Role of mechanotransduction in stem cells and cancer progression

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