Recent Advances in Hormonal Induction of Sex-Reversal in Fish

Pandian, T. J.; Santhakumar, Kirankumar

doi:10.1300/j028v13n03_02

Cited by 49 publications

(29 citation statements)

References 81 publications

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“…George and Pandian, '96). Hormone administration through discrete immersion technique has been more widely practiced during recent years, as it is equally effective (e.g., Piferrer et al, '94), cheaper cost-wise, and less polluting (Pandian and Kirankumar, 2002). This research work was undertaken to understand the causative factors responsible for low survival, delayed sexual maturity, and reduced fecundity of hormonally sex-reversed fish and to find out whether the…”

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confidence: 99%

Effect on growth and reproduction of hormone immersed and masculinized fighting fish Betta splendens

Santhakumar

Pandian

2002

J. Exp. Zool.

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To produce all-male progenies in the fighting fish, Betta splendens, six groups of fry were subjected to discrete immersion treatment at different 17alpha-methyltestosterone (MT) doses (viz. 100, 200, 500, 700, 900, and 1,000 microg/l) for a constant duration (3 hr/day) and frequency (second, fifth, and eighth day after hatching). The treatment at 900 microg/l led to 98% masculinization and 71% survival at sexual maturity. Treated groups, which showed significant deviation from the 1:1 sex ratio, were classified into two different series: S1 and S2. The groups that showed nearly cent-percent masculinization were classified as S1, and the other groups were classified as S2. The S1 males showed remarkably slower growth and attained 3.5 cm total length compared to 6.0 cm attained by a normal male. The S2 males attained 5.4 cm total length. Apart from these morphological defects, both S1 and S2 males suffered functional (decreased sperm count and sperm motility) and behavioral defects (incomplete embracing during mating) in their reproductive ability, leading to approximately 50% and 30% reduction in fecundity per mating, respectively. The cumulative fecundity loss suffered by the S1 male during its active reproductive phase is discussed. When normal and sex-reversed males were presented, a female preferred the former. Progeny testing of the sex-reversed males showed the occurrence of 12.75% males, indicating the possible role of autosomal genes in the sex determination mechanism of this species. Discrete immersion treatment at optimal/super-optimal doses ensured not only a higher percentage of masculinization, but also a higher frequency of homogametic males (XX).

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Effect on growth and reproduction of hormone immersed and masculinized fighting fish Betta splendens

Santhakumar

Pandian

2002

J. Exp. Zool.

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“…Genome manipulation (for reviews see Felip et al 2001;Komen & Thorgaard 2007) and hormonal sex reversal experiments (for review see Pandian & Kirankumar 2003) were used to obtain indications about the presence of strong sex chromosomal systems. In four groups of gynogenetic turbot individuals the proportion of females was found to be 69, 75, 90 and 100% (Baynes et al 2006;Cal et al 2006b).…”

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confidence: 99%

Sex-associated DNA markers from turbot

Casas¹,

Sánchez²,

Orbán³

2011

Marine Biology Research

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The genomes of male and female turbot, Psetta maxima (Linnaeus, 1758), were screened for sex-specific sequences by comparative random amplified polymorphic DNA (RAPD) assays performed on pooled samples. As turbot females grow much faster than males, there is an increasing interest in differentiating between the sexes, and such markers would enable the sexing of individuals even at early stages of development. Four sex-associated DNA markers, with molecular sexing efficiencies ranging from 77 to 90%, were identified and further characterized. Two markers were identified from pooled female samples, one from pooled male samples (both sets collected from the wild), whereas a fourth marker was found in the female pool of farm-bred siblings. Parallel application of the three markers isolated from wild-caught individuals yielded a combined molecular sexing efficiency of 90% in males and 83.3% in females. While the three markers isolated from the wild should be useful to predict the sex in natural turbot populations, the fourth marker showed sex-association limited only to the family where it was isolated from. Nonetheless, it can be used for molecular sexing of brooders originating from the target family and potentially their offspring as well. The application of such molecular sex markers with limited scope is a potential strategy in aquaculture for those species for which no universal sex marker is available.

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“…Pandian and Kirankumar (2003) stated that hormonally sex-reversed fish suffered from low survival and functional deficiencies. However, in these experiments, all dead fish were weak and thin, indicating that the probable cause of mortality was starvation.…”

Section: Discussionmentioning

confidence: 99%

Progress in Studies on Hormonal Sex Reversal and Genetic Sex Control in Black Crappie

Cuevas‐Uribe

Gomelsky

Mims

et al. 2009

Reviews in Fisheries Science

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Sex control can solve the problem of stunted black crappie populations in small impoundments. The main objectives of the present study were (1) to identify sex-reversed males of black crappie from a previously obtained androgen-treated group using test crosses, and (2) to develop broodstock of sex-reversed males by masculinization of fish from those crosses. An additional objective of the study was to try to identify sex-specific RAPD markers, which might be used for identification of sex-reversed males. The progenies resulting from test crosses were divided into two groups: Group I (control) fish were raised without hormonal treatment, while Group II fish were subjected to androgen (MT) treatment. Seven progenies were obtained from the cross of preliminary androgen-treated males with normal females. Six of seven progenies had sex ratio in Groups I close to 1:1, and one progeny consisted of females only. This shows that only one male, which generated this progeny, was a sex-reversed homogametic fish (XX) while the other males were heterogametic normal (XY) fish. In Group II the androgen 17α-methyltestosterone (MT) was orally administered to crappie with an artificial diet (30 mg/kg) for 40 days beginning 35 days post hatching; androgen-treated groups consisted of 95-100% males. Sex-specific random-amplified polymorphic DNA markers were not identified in black crappie.

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Recent Advances in Hormonal Induction of Sex-Reversal in Fish

Cited by 49 publications

References 81 publications

Effect on growth and reproduction of hormone immersed and masculinized fighting fish Betta splendens

Effect on growth and reproduction of hormone immersed and masculinized fighting fish Betta splendens

Sex-associated DNA markers from turbot

Progress in Studies on Hormonal Sex Reversal and Genetic Sex Control in Black Crappie

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