Recent advances in gold nanoparticles for biomedical applications: from hybrid structures to multi-functionality

Jeong, Hyeon‐Ho; Choi, Eun‐Mi; Ellis, Elizabeth M.; Lee, Tung‐Chun

doi:10.1039/c9tb00557a

Cited by 122 publications

(100 citation statements)

References 187 publications

(232 reference statements)

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“…Gold nanoparticles are arguably the most versatile nanomaterials reported up to now [45]. Due to the biological and chemical properties, their wide applications range from drug delivery to cancer therapy [46][47][48].…”

Section: Discussionmentioning

confidence: 99%

Proteome interrogation using gold nanoprobes to identify targets of arctigenin in fish parasites

Tan

et al. 2020

J Nanobiotechnol

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Gold nanoparticles (GNPs) are one of the most widely used nanomaterials in various fields. Especially, the unique chemical and physical properties make them as the promising candidates in drug target identification, unfortunately, little is known about their application in parasites. In this paper, GNPs were employed as new solid support to identify drug targets of natural bioactive compound arctigenin (ARG) against fish monogenean parasite Gyrodactylus kobayashi. Before target identification, GNPs with ARG on the surface showed the ability to enter the live parasites even the nucleus or mitochondria, which made the bound compounds capable of contacting directly with target proteins located anywhere of the parasites. At the same time, chemically modified compound remained the anthelminthic efficacy against G. kobayashii. The above results both provide assurance on the reliability of using GNPs for drug target-binding specificity. Subsequently, by interrogating the cellular proteome in parasite lysate, myosin-2 and UNC-89 were identified as the potential direct target proteins of ARG in G. kobayashii. Moreover, results of RNA-seq transcriptomics and iTRAQ proteomics indicated that myosin-2 expressions were down-regulated after ARG bath treatment both in transcript and protein levels, but for UNC-89, only in mRNA level. Myosin-2 is an important structural muscle protein expressed in helminth tegument and its identification as our target will enable further inhibitor optimization towards future drug discovery. Furthermore, our findings demonstrate the power of GNPs to be readily applied to other parasite drugs of unknown targets, facilitating more broadly therapeutic drug design in any pathogen or disease model.

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Section: Discussionmentioning

confidence: 99%

Proteome interrogation using gold nanoprobes to identify targets of arctigenin in fish parasites

Tan

et al. 2020

J Nanobiotechnol

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Section: General Considerations Of Gold Nanoparticlesmentioning

confidence: 99%

“…The use of stabilizing agents is necessary to prevent the aggregation of the gold particles. The state-of-the-art in AuNP synthesis has been thoroughly reviewed in the comprehensive work by Zhao et al [8] and in some recent publications [9,10]. Several varieties of nanoparticles with biomedical relevance have been developed in recent years, including metal nanoparticles, polymeric nanoparticles, liposomes, micelles, quantum dots, and dendrimers.…”

Section: General Considerations Of Gold Nanoparticlesmentioning

confidence: 99%

Gold Nanoparticles: A New Golden Era in Oncology?

et al. 2020

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In recent years, the spectrum of possible applications of gold in diagnostics and therapeutic approaches in clinical practice has changed significantly, becoming surprisingly broad. Nowadays, gold-based therapeutic agents are used in the therapy of multiple human diseases, ranging from degenerative to infectious diseases and, in particular, to cancer. At the basis of these performances of gold, there is the development of new gold-based nanoparticles, characterized by a promising risk/benefit ratio that favors their introduction in clinical trials. Gold nanoparticles appear as attractive elements in nanomedicine, a branch of modern clinical medicine, which combines high selectivity in targeting tumor cells and low toxicity. Thanks to these peculiar characteristics, gold nanoparticles appear as the starting point for the development of new gold-based therapeutic strategies in oncology. Here, the new gold-based therapeutic agents developed in recent years are described, with particular emphasis on the possible applications in clinical practice as anticancer agents, with the aim that their application will give rise to a new golden age in oncology and a breakthrough in the fight against cancer.

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“…GNPs in complexes with bioreceptor molecules (antibodies, aptamers, etc.) are used as analytical markers [11,12]. To obtain nanoconjugates, a large number of covalent sewing techniques have been developed, but for biological macromolecules, physical adsorption methods remain the most popular [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

A Mechanism of Gold Nanoparticle Aggregation by Immunoglobulin G Preparation

et al. 2020

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Conjugates of gold nanoparticles (GNPs) and antibodies are widely used in various fields of biochemistry and microbiology. However, the procedure for obtaining such conjugates remains precarious, and the properties of conjugates differ significantly for different antibody clones. One of the most common problems is the aggregation of GNPs in the course of their conjugation with antibodies. This article considers an example of the conjugation of monoclonal antibodies with non-stable aggregating product. The composition of the antibody preparation was studied using electrophoresis, asymmetrical flow field-flow fractionation, and ultracentrifugation. It was shown that the component that causes the aggregation of the GNPs is the light chains of immunoglobulins that appear due to the spontaneous decay of the antibodies. After separation of the fraction with a molecular weight of less than 30 kDa, stable conjugates of antibodies with GNPs were obtained. The high functional activity of the obtained conjugates was confirmed by immunochromatography.

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Recent advances in gold nanoparticles for biomedical applications: from hybrid structures to multi-functionality

Abstract: Hybrid gold nanoparticles for biomedical applications are reviewed in the context of a novel classification framework and illustrated by recent examples.

Cited by 122 publications

References 187 publications

Proteome interrogation using gold nanoprobes to identify targets of arctigenin in fish parasites

Proteome interrogation using gold nanoprobes to identify targets of arctigenin in fish parasites

Gold Nanoparticles: A New Golden Era in Oncology?

A Mechanism of Gold Nanoparticle Aggregation by Immunoglobulin G Preparation

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