Recent advances in gold nanoparticle-based lateral flow immunoassay for the detection of bacterial infection

Gupta, Yachana; Ghrera, Aditya Sharma

doi:10.1007/s00203-021-02357-9

Cited by 35 publications

(19 citation statements)

References 112 publications

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“…Linear proportionality of this plot indicates that magnitude of current changes linearly with the change in analyte concentration, while the negative slope of this plot indicates that oxidation current decreases with the increase of analyte on the paper strip. The linear proportionality of the current value and PCT concentration in the range from 1 to 60 ng ml -1 corresponds to the equation (1), showing the regression coefficient value of 0.97 and sensitivity value of 0.695 A ml ng -1 .…”

Section: Electrochemical Characterization Of Lfa Strip and Response Studiesmentioning

confidence: 80%

“…According to WHO report, antibiotic-resistant bacteria pose a risk to public health. Therefore, for BI's diagnosis and immediate response, some on-site detection technique is required [1]. For the clinical utility of BI specific biomarkers, numerous immunoassays like time-resolved fluoro-immunoassay (TRFIA) [2,3], chemiluminescence immunoassays (CLIA) [4], immunochromatographic tests (ICT), and enzyme-linked immunosorbent assays (ELISA) [5] have been presented in a wide variety of research applications for diagnosis purposes.…”

Section: Introductionmentioning

confidence: 99%

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Electrochemical studies of lateral flow assay test results for procalcitonin detection

Gupta¹,

Kalpana²,

Ghrera³

2021

J. Electrochem. Sci. Eng.

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In this study, the lateral flow assay (LFA) has been developed for the detection of bacterial infection (BI) by specific biomarker procalcitonin (PCT), without a need for complicated instrumentations and technical expertise. For the development of the assay, gold nanoparticles (AuNP) and their conjugates with antibodies specific to the model antigen PCT are assessed. Polyclonal antibody (pAb) labelled with gold nanoparticles (AuNP) to obtain the AuNP-pAb complex and the specific monoclonal antibody (mAb) have been dropped at the test zone. This complex is placed over the conjugate line of the LFA strip. In the absence of PCT or the presence of other biomarkers, the test line remained colourless, which revealed the specificity of assay towards PCT among a pool of various analytes. Herein, observations have been made through two different platforms for quantitative and qualitative analysis for the detection of PCT biomarker. The qualitative analysis has been performed on the basis of appearance red color in the test band, while for quantitative analysis, a novel approach has been adopted. Herein, the nitrocellulose membrane (paper strip) is cut out from the LFA strip and used for electrochemical studies under similar solution conditions. Different paper strips presented different cyclic voltammograms (CV) that could be correlated to varying PCT concentrations captured at the test line of the paper strip. The qualitative detection limit for PCT using this LFA was determined to be 2 ng/ml and the quantitative detection limit was 1 ng/ml. The electrochemical response studies of the paper strip by CV technique revealed the sensitivity value of 0.695 mA ng/ml.

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Section: Electrochemical Characterization Of Lfa Strip and Response Studiesmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Electrochemical studies of lateral flow assay test results for procalcitonin detection

Gupta¹,

Kalpana²,

Ghrera³

2021

J. Electrochem. Sci. Eng.

Self Cite

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“…The sandwich format is used to detect large targets that have at least two epitopes (binding sites). In contrast, the competitive format is used to detect small targets with a single epitope [ 27 ].…”

Section: Lateral Flow Immunoassaymentioning

confidence: 99%

Quantum Dot-Based Lateral Flow Immunoassay as Point-of-Care Testing for Infectious Diseases: A Narrative Review of Its Principle and Performance

et al. 2022

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Infectious diseases are the world’s greatest killers, accounting for millions of deaths worldwide annually, especially in low-income countries. As the risk of emerging infectious diseases is increasing, it is critical to rapidly diagnose infections in the early stages and prevent further transmission. However, current detection strategies are time-consuming and have exhibited low sensitivity. Numerous studies revealed the advantages of point-of-care testing, such as those which are rapid, user-friendly and have high sensitivity and specificity, and can be performed at a patient’s bedside. The Lateral Flow Immunoassay (LFIA) is the most popular diagnostic assay that fulfills the POCT standards. However, conventional AuNPs-LFIAs are moderately sensitive, meaning that rapid detection remains a challenge. Here, we review quantum dot (QDs)-based LFIA for highly sensitive rapid diagnosis of infectious diseases. We briefly describe the principles of LFIA, strategies for applying QDs to enhance sensitivity, and the published performance of the QD-LFIA tested against several infectious diseases.

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“…Antibiotics are widely used in treating human and animal infections due to their unique antibacterial properties. 1 However, since most antibiotics are challenging for organisms to fully digest, they and their metabolites are discharged into the environment through excretions and urine. 2,3 Even though antibiotic concentrations are oen detected at trace levels in the environment, they can still induce antibiotic resistance genes at a low concentration.…”

Section: Introductionmentioning

confidence: 99%

An efficient removal mechanism for different hydrophilic antibiotics from aquatic environments by Cu–Al–Fe–Cr quasicrystals

Zhang

Tian

et al. 2022

RSC Adv.

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Recent advances in gold nanoparticle-based lateral flow immunoassay for the detection of bacterial infection

Cited by 35 publications

References 112 publications

Electrochemical studies of lateral flow assay test results for procalcitonin detection

Electrochemical studies of lateral flow assay test results for procalcitonin detection

Quantum Dot-Based Lateral Flow Immunoassay as Point-of-Care Testing for Infectious Diseases: A Narrative Review of Its Principle and Performance

An efficient removal mechanism for different hydrophilic antibiotics from aquatic environments by Cu–Al–Fe–Cr quasicrystals

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