Recent advances in gold-based metal enhanced fluorescence platforms for diagnosis and imaging in the near-infrared

Joyce, Caoimhe; Fothergill, Sarah Madeline; Xie, Fang

doi:10.1016/j.mtadv.2020.100073

Cited by 38 publications

(33 citation statements)

References 97 publications

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“…Such quantitative studies on the plasmon-enhanced fluorescence have led to the development of metal-enhanced fluorescence imaging (Figure B), biosensing (Figure C), and therapeutic devices . PEF bio applications techniques are primarily focused on substrate-based PEF and more recently has explored solution-based enhancement approaches . Among several materials and architectures, NPG has been extensively explored for biosensing and imaging applications, owing to its physical properties such as excellent stability, biocompatibility, and high specific surface area to form self-assembled monolayers from thiols. ,, Particularly, Ahmed et al have demonstrated a rapid, sensitive and quantitative detection of influenza A virus using a hybrid structure of quantum dot (QD) and nanoporous gold leaf (NPGL) .…”

Section: Nanoporous Metals For Enhanced Spectroscopymentioning

confidence: 99%

Nanoporous Metals: From Plasmonic Properties to Applications in Enhanced Spectroscopy and Photocatalysis

et al. 2021

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The field of plasmonics is capable of enabling interesting applications in different wavelength ranges, spanning from the ultraviolet up to the infrared. The choice of plasmonic material and how the material is nanostructured has significant implications for ultimate performance of any plasmonic device. Artificially designed nanoporous metals (NPMs) have interesting material properties including large specific surface area, distinctive optical properties, high electrical conductivity, and reduced stiffness, implying their potentials for many applications. This paper reviews the wide range of available nanoporous metals (such as Au, Ag, Cu, Al, Mg, and Pt), mainly focusing on their properties as plasmonic materials. While extensive reports on the use and characterization of NPMs exist, a detailed discussion on their connection with surface plasmons and enhanced spectroscopies as well as photocatalysis is missing. Here, we report on different metals investigated, from the most used nanoporous gold to mixed metal compounds, and discuss each of these plasmonic materials’ suitability for a range of structural design and applications. Finally, we discuss the potentials and limitations of the traditional and alternative plasmonic materials for applications in enhanced spectroscopy and photocatalysis.

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Section: Nanoporous Metals For Enhanced Spectroscopymentioning

confidence: 99%

Nanoporous Metals: From Plasmonic Properties to Applications in Enhanced Spectroscopy and Photocatalysis

et al. 2021

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“…Metal enhanced fluorescence (MEF) is a phenomenon in which strong surface plasmons of the metal enhances the fluorescence and Raman signals of molecules adsorbed close to the metal surface (Fig. 1 a) [ 23 ]. In a study examining AuNP carrying PSs [ 24 , 25 ], fluorescence of conjugated Ce6 on the AuNPs surface was enhanced threefold, and ROS production was increased 1.4-fold.…”

Section: Introductionmentioning

confidence: 99%

A novel therapeutic strategy of multimodal nanoconjugates for state-of-the-art brain tumor phototherapy

2022

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Background The outcome of phototherapy, including photothermal therapy (PTT) and photodynamic therapy (PDT) for glioblastoma multiforme (GBM), is disappointing due to insufficient photoconversion efficiency and low targeting rate. The development of phototherapeutic agents that target GBM and generate high heat and potent ROS is important to overcome the weak anti-tumor effect. Results In this study, nanoconjugates composed of gold nanoparticles (AuNPs) and photosensitizers (PSs) were prepared by disulfide conjugation between Chlorin e6 (Ce6) and glutathione coated-AuNP. The maximum heat dissipation of the nanoconjugate was 64.5 ± 4.5 °C. Moreover, the proximate conjugation of Ce6 on the AuNP surface resulted in plasmonic crossover between Ce6 and AuNP. This improves the intrinsic ROS generating capability of Ce6 by 1.6-fold compared to that of unmodified-Ce6. This process is called generation of metal-enhanced reactive oxygen species (MERos). PEGylated-lactoferrin (Lf-PEG) was incorporated onto the AuNP surface for both oral absorption and GBM targeting of the nanoconjugate (denoted as Ce6-AuNP-Lf). In this study, we explored the mechanism by which Ce6-AuNP-Lf interacts with LfR at the intestinal and blood brain barrier (BBB) and penetrates these barriers with high efficiency. In the orthotopic GBM mice model, the oral bioavailability and GBM targeting amount of Ce6-AuNP-Lf significantly improved to 7.3 ± 1.2% and 11.8 ± 2.1 μg/kg, respectively. The order of laser irradiation, such as applying PDT first and then PTT, was significant for the treatment outcome due to the plasmonic advantages provided by AuNPs to enhance ROS generation capability. As a result, GBM-phototherapy after oral administration of Ce6-AuNP-Lf exhibited an outstanding anti-tumor effect due to GBM targeting and enhanced photoconversion efficiency. Conclusions The designed nanoconjugates greatly improved ROS generation by plasmonic crossover between AuNPs and Ce6, enabling sufficient PDT for GBM as well as PTT. In addition, efficient GBM targeting through oral administration was possible by conjugating Lf to the nanoconjugate. These results suggest that Ce6-AuNP-Lf is a potent GBM phototherapeutic nanoconjugate that can be orally administered. Graphical Abstract

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“…Fluorescence intensity and fluorophore photostability can be influenced by taking advantage of different metals, compositions, and geometries (Badshah et al., 2020). One of the main metals used in biological applications is gold (Joyce, Fothergill, & Xie, 2020; Niu, Song, He, Na, & Ouyang, 2016).…”

Section: Introductionmentioning

confidence: 99%

Methods for Enhanced Fluorescence Detection of Proteins by using Entrapped Gold Nanoparticles in Membranes

et al. 2022

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Measuring protein levels from biofluids can provide important insight into human health and disease during various physiological and pathological conditions. In many situations, sensitive methods are required for protein quantification because at the early stages of many diseases, proteins in biofluids are present at very low concentrations. Here, a new and simple method is presented in the form of Basic and Alternative Protocols for an immunoassay performed on a nitrocellulose membrane, followed by the addition of gold nanoparticles prior to measuring fluorescence with a microscope. The assay protocol was optimized to achieve 3D metal‐enhanced fluorescence (MEF) with increased antibody‐binding capacity and enhanced fluorescence signals, improving assay sensitivity. Using different concentrations of spiked fluorescently labeled IgGs in pooled normal human plasma, a lower detection limit of 29 ng/ml was achieved. This limit of detection was found to be a thousand‐fold lower than the conventional 2D assay and one order of magnitude lower than when the assay was performed on a 3D membrane without MEF. This method provides an easy way to improve immunoassay sensitivity, and it can be simply transferred to other labs. It also can extend to fluorescence detection of other analytes beyond proteins. © 2022 Wiley Periodicals LLC. Basic Protocol: Assay in nitrocellulose membrane with entrapped AuNPs using commercially available AuNPs Alternative Protocol: Assay in nitrocellulose membrane with entrapped AuNPs using lab‐made AuNPs

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Recent advances in gold-based metal enhanced fluorescence platforms for diagnosis and imaging in the near-infrared

Cited by 38 publications

References 97 publications

Nanoporous Metals: From Plasmonic Properties to Applications in Enhanced Spectroscopy and Photocatalysis

Nanoporous Metals: From Plasmonic Properties to Applications in Enhanced Spectroscopy and Photocatalysis

A novel therapeutic strategy of multimodal nanoconjugates for state-of-the-art brain tumor phototherapy

Methods for Enhanced Fluorescence Detection of Proteins by using Entrapped Gold Nanoparticles in Membranes

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