Recent Advances in Fruit Species Transformation

Akdemir, Hülya; Gago, Jorge; Gallego, Pedro Pablo Ferrer; Çiftçi, Yelda Özden

doi:10.5772/35139

Cited by 2 publications

(2 citation statements)

References 201 publications

(168 reference statements)

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“…Many of the species and cultivars of apple perform poorly towards the development of transgenic tissues because of inefficacious regeneration system (Akdemir et al, 2012. Increased leaf regeneration efficiency remains to be the most imperative factor for development of a transformation system in apple while using A. tumefaciens vectors.…”

Section: Discussionmentioning

confidence: 99%

IN VITRO REGENERATION AND TRANSFORMATION OF APPLE (Malus domestica Borkh.) ROOTSTOCK MALLING 7 USING RICE CHITINASE GENE

Sharma¹,

Modgil²,

Sharma³

2017

JEBAS

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In most of the fruit crops, the low frequency of Agrobacterium mediated genetic transformation hampers the respective transgenics production. Hence, it is pre-requisite to understand and optimize the different parameters involved directly or indirectly in Agrobacterium mediated transformation. A. tumefaciens strain LBA4404 harboring the transforming vector pCAMBARchi11 containing the rice chitinase gene (chi11) and hygromycin phosphotransferase (hpt) and phosphinothricin acetyl transferase (bar) genes was used for carrying out genetic transformation of apple (Malus x domestica Borkh.) rootstock M7. Two explants viz. leaf and internodal segments were involved in regeneration and resulted 24.37% and 60.58% shoot regeneration, respectively. The obtained putative transformants were selected on full strength MS medium containing 5 mg l -1 hygromycin and 500 mg l -1 cefotaxime. Two days pre-culturing and 96 hours co-cultivation was found effective for procuring maximum number of hygromycin resistant shoots. Putative transgenic shoots were multiplied separately and rooted on root induction medium containing 5 mg l -1 hygromycin. Further, PCR analysis was done using chitinase gene specific primers and two apple transgenic lines confirmed the integration of chi11 gene by yielding 237 bp and 584 bp amplified products, respectively.

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Section: Discussionmentioning

confidence: 99%

IN VITRO REGENERATION AND TRANSFORMATION OF APPLE (Malus domestica Borkh.) ROOTSTOCK MALLING 7 USING RICE CHITINASE GENE

Sharma¹,

Modgil²,

Sharma³

2017

JEBAS

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“…As a perennial plantation crop the use of continuous groundwater irrigation is commonplace but can lead to build-up mineral salts in the rooting zone reducing the production potential (Tóth et al 2008). (Flaishman et al 2008a;Akdemir et al 2012). Genetic and transformation methodologies offer the opportunity for the production of transgenic cultivars with improved agronomic characteristics, such as disease resistance, fruit storability, and enhanced fruit quality and flavour (Azafadi 2012;Meneses and Orellana 2013;Bakhsh and Hussain 2015).…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning and expression of a vacuolar Na+/H+ antiporter gene (AgNHX1) in fig (Ficus carica L.) under salt stress

Metwali

Soliman

Fuller

et al. 2015

Plant Cell Tiss Organ Cult

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Soil salinity can be a major limiting factor for productivity in agriculture and forestry and in order to fully utilize saline lands productively in plantation forestry for fig production, the genetic modification of tree species for salt tolerance may be required. Na ? /H ? antiporters have been suggested to play important roles in salt tolerance in plants.Here, we isolated AgNHX1 a vacuolar Na ? /H ? antiporter from a halophytic species Atriplex gmelini and introduced it into fig (Ficus carica L.) cv. Black Mission via Agrobacterium-mediated transformation. Leaf discs explants of fig were co-cultivated for 2 days with Agrobacterium tumefaciens strain LBA 4404 harboring the binary vector pBI121 containing the AgNHX1 gene and the hpt selectable marker gene which encodes hygromycin phosphotransferase. Explants were cultured on MS medium containing 30 mg L -1 hygromycin, 3 % sucrose, 0.2 mg L -1 kinetin and 2.0 mg L -1 2,4-dichlorophenoxyacetic acid solidified with 2.5 g L -1 phytagel in darkness for callus formation. The calli were cultured on MS medium containing 2.0 mg L -1 zeatin riboside in combination with 0.4 mg L -1 indole acetic acid in the light for plant regeneration. Putative regenerated transformant shoots were confirmed by polymerase chain reaction (PCR) and Southern hybridization for the AgNHX1 gene. Reverse transcriptase polymerase chain reaction analysis indicated that the gene was highly expressed in transgenic plants, but the degree of this expression varied among transformants. Overexpression of the AgNHX1 gene conferred high tolerance to salt stress and transgenic fig plants overexpressing AgNHX1 developed normally under salinity conditions compared to those of non-transgenic plants. Salt treated transgenic plants contained high proline and K ? but less Na ? compared to non-transgenic control plants.

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Recent Advances in Fruit Species Transformation

Cited by 2 publications

References 201 publications

IN VITRO REGENERATION AND TRANSFORMATION OF APPLE (Malus domestica Borkh.) ROOTSTOCK MALLING 7 USING RICE CHITINASE GENE

IN VITRO REGENERATION AND TRANSFORMATION OF APPLE (Malus domestica Borkh.) ROOTSTOCK MALLING 7 USING RICE CHITINASE GENE

Molecular cloning and expression of a vacuolar Na+/H+ antiporter gene (AgNHX1) in fig (Ficus carica L.) under salt stress

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