Recent advances in enzyme immobilization techniques: Metal-organic frameworks as novel substrates

Mehta, Jyotsana; Bhardwaj, Neha; Bhardwaj, Sanjeev K.; Kim, Ki‐Hyun; Deep, Akash

doi:10.1016/j.ccr.2016.05.007

Cited by 377 publications

(183 citation statements)

References 109 publications

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“…However, the applications of enzymes in industry are limited because of the high cost, specific reaction conditions and the fragile nature of enzymes themselves. To overcome these problems, there are two main directions: 1) synthesizing biocompatible materials to mimic the functions of nature enzymes; and 2) stabilizing nature enzymes into unnatural materials to remain their functions and activities . Up to now, bulk MOFs have already been reported in both two directions .…”

Section: Artificial Enzymesmentioning

confidence: 99%

Two‐Dimensional Metal‐Organic Framework Nanosheets: A Rapidly Growing Class of Versatile Nanomaterials for Gas Separation, MALDI‐TOF Matrix and Biomimetic Applications

Yang

2018

Chemistry A European J

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Two-dimensional (2D) metal-organic framework (MOF) nanosheets, as an emerging type of 2D materials, attract numerous attention due to their unique properties. First, the ultrathin thickness and nanoscale of the materials results in homogeneous dispersion in aqueous solution, giving the materials more opportunities to be utilized in solution chemistry, especially beneficial to the biomimetic catalysis and bio-related analytical applications. Second, the large surface area and accessible active sites of the MOF nanosheets are favorable to the binding between materials and the substrate, leading to their superior performance in catalysis, sensing and enzyme inhibition. Third, the suitable sizes of nanopores on the 2D MOF nanosheets give them the abilities to act as membranes for highly selective and energy-saving gas separation. This minireview covers the synthesis, characterization as well as bio-related and separation applications of 2D MOF nanosheets.

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Section: Artificial Enzymesmentioning

confidence: 99%

Two‐Dimensional Metal‐Organic Framework Nanosheets: A Rapidly Growing Class of Versatile Nanomaterials for Gas Separation, MALDI‐TOF Matrix and Biomimetic Applications

Yang

2018

Chemistry A European J

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“…金属有机骨架材料(MOFs)作为一种无机-有机纳米晶体材料, 因其具有多孔骨架结构, 比表面积大, 化学稳定性好, 热稳定性好等优势, 近年来开始成为新型的固相载体材料用于酶的固定化而备受青睐, 这也为 MOFs 材料的应用开辟了新的领域 [5,6] .…”

Section: 引言unclassified

Immobilization of Chloroperoxidase in Metal Organic Framework and Its Catalytic Performance

Zhao¹,

Hu²,

Li³

et al. 2017

Acta Chim. Sinica

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A rapid and efficient preparation of CPO@ZIF-8 by "one pot " method at 30 ℃ in aqueous solution is presented in this paper. The structure of zeolitic imidazolate frameworks (ZIF-8) was constructed while chloroperoxidase (CPO) was incorporated into the channel. Mild reaction conditions ensure maintaining the enzyme activity in the preparation of immobilized CPO. The synthesis of CPO@ZIF-8 was performed by mixing zinc nitrate solution and polyvinylpyrrolidone solution (PVP, M w : 10000, 10 mg/mL, 400 μL), chloroperoxidase (CPO) (0.214 mmol/L, 500 μL) and 2-methylimidazole (1.25 mol/L, 25 mL) and stirring for 15 min at 30 ℃, followed by washing and centrifuging for 3 cycles at 4 ℃ for 8 min. The structure of CPO@ZIF-8 was characterized by scanning electron microscope (SEM), transmission electron microscopy (TEM) and X-ray diffraction (XRD), indicating that the incorporation of enzyme molecules did not affect the crystal structure of ZIF-8. To further confirm the incorporation of enzyme into ZIF-8, CPO was labeled by fluorescent probes fluorescein isothiocyanate (FITC) and subjected to the same procedure to synthesize the FITC-CPO@ZIF-8. Confocal laser scanning microscopy (CLSM) proved that CPO was distributed evenly and embedded in the whole framework of CPO@ZIF-8. Compared with the method of preparing ZIF-8 firstly, and then immobilizing enzyme molecule by physical adsorption, the immobilization efficiency of enzyme was enhanced by introducing the enzyme into the whole framework, moreover, the catalytic efficiency of the immobilized CPO was increased due to high specific surface area of ZIF-8. The catalytic performance of the CPO@ZIF-8 was evaluated by the conversion rate of 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS). The rigid shielding environment provided by the three-dimensional channel of ZIF-8 effectively improved the thermal stability, pH stability, and tolerance to organic solvents of the CPO@ZIF-8 under harsh reaction conditions compared with the free enzyme. When incubated at 50 ℃, 60 ℃, 70 ℃, 80 ℃ and 90 ℃ for 1 h, 97.1%, 87.8%, 80.2%, 68.1% and 41.5% of the activity of CPO@ZIF-8 were reserved. When incubated at 50 ℃, 60 ℃, 70 ℃ and 80 ℃ for 3 h, there were still 91.4%, 77.8%, 64.7% and 50.3% of the activity remained. The tolerance of CPO@ZIF-8 to organic solvent DMF, methanol and methyl cyanide was enhanced to 30%～40%.

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“…Zinc cation has a distorted tetrahedral coordination environment built by two N atoms of two C5 ligands and two O atoms of two bdc 2− anions ( Figure 1). Zn-N bond lengths are 1.9987 (15) (Figure 1). Zinc cations are interconnected via bridging bdc 2− and C5 ligands to form a metal-organic framework ( Figure 2) with a diamond topology (dia) while the overall crystal structure of both 1 and 2 results from a triple interpenetration of such nets (Figure 3) (Figure 1).…”

Section: Crystal Structures Of Compounds 1-4mentioning

confidence: 99%

Section: Crystal Structures Of Compounds 1-4mentioning

confidence: 99%

“…Ongoing research aimed at the design of new metal-organic frameworks (MOFs) is stimulated by their high capacity for gas storage and separation [1][2][3][4][5][6] photo-physical properties [7][8][9][10], sensor capabilities [11], excellent catalytic activity [12,13], medical imaging and drug delivery applications [14,15]. Functional properties of MOFs can be fine-tuned by careful choice of organic linkers, both rigid and flexible ligands are suitable for this aim [16].…”

Section: Introductionmentioning

confidence: 99%

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Synthesis, Crystal Structure, and Luminescent Properties of New Zinc(II) and Cadmium(II) Metal-Organic Frameworks Based on Flexible Bis(imidazol-1-yl)alkane Ligands

et al. 2016

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New metal-organic frameworks (MOFs) based on zinc and cadmium ions, terephthalic acid, and flexible ligands 1,5-bis(imidazol-1-yl)pentane or 1,6-bis(imidazol-1-yl)hexane were prepared and characterized by X-ray diffraction, thermorgavimetric analysis and IR spectroscopy. The imidazolyl ligands were prepared by a new robust procedure involving the reaction between imidazole and 1,5-dibromopentane or 1,6-dibromohexane in a superbasic medium (KOH in DMSO). MOFs based on 1,5-bis(imidazol-1-yl)pentane had diamond topology (dia) and are triply interpenetrated. Ligands with longer spacer 1,6-bis(imidazol-1-yl)hexane, terephthalate ions and zinc(II) ions formed five-fold interpenetrated metal-organic framework also with dia topology, while cadmium(II) ions with the same ligands formed eight-connected uninodal net with a very rare self-penetrated topological type ilc and a point symbol 4 24 .5.6 3 . The influence of the chemical composition of MOFs on their photoluminescent properties is investigated and discussed in detail.

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Recent advances in enzyme immobilization techniques: Metal-organic frameworks as novel substrates

Cited by 377 publications

References 109 publications

Two‐Dimensional Metal‐Organic Framework Nanosheets: A Rapidly Growing Class of Versatile Nanomaterials for Gas Separation, MALDI‐TOF Matrix and Biomimetic Applications

Two‐Dimensional Metal‐Organic Framework Nanosheets: A Rapidly Growing Class of Versatile Nanomaterials for Gas Separation, MALDI‐TOF Matrix and Biomimetic Applications

Immobilization of Chloroperoxidase in Metal Organic Framework and Its Catalytic Performance

Synthesis, Crystal Structure, and Luminescent Properties of New Zinc(II) and Cadmium(II) Metal-Organic Frameworks Based on Flexible Bis(imidazol-1-yl)alkane Ligands

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