Recent advances in enzyme immobilization based on novel porous framework materials and its applications in biosensing

Feng, Yu; Xu, Ying; Liu, Shucheng; Wu, Di; Su, Zhuoqun; Chen, Guang; Liu, Jianghua; Li, Guoliang

doi:10.1016/j.ccr.2022.214414

Cited by 150 publications

(97 citation statements)

References 181 publications

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“…Colorimetry as one of the most common signal modes has been widely applied in the establishment of biosensors due to its merits of easy read-out, convenient operation, and fast visual measurement through the portable mini-equipment (e.g., smartphone) and even naked eyes. [58][59][60] Colorimetric biosensor as an optical sensor is used for detecting analytes mainly based on the color change during the biochemical reaction induced by analytes. [61,62] Encouraged by its numerous advantages, colorimetric sensing as a very popular sensing model has been used in the construction of CRISPR/Cas systems-based bio/nanosensing.…”

Section: Colorimetric Bio/nanosensingmentioning

confidence: 99%

CRISPR/Cas Systems‐Inspired Nano/Biosensors for Detecting Infectious Viruses and Pathogenic Bacteria

et al. 2022

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Infectious pathogens cause severe human illnesses and great deaths per year worldwide. Rapid, sensitive, and accurate detection of pathogens is of great importance for preventing infectious diseases caused by pathogens and optimizing medical healthcare systems. Inspired by a microbial defense system (i.e., CRISPR/ CRISPR-associated proteins (Cas) system, an adaptive immune system for protecting microorganisms from being attacked by invading species), a great many new biosensors have been successfully developed and widely applied in the detection of infectious viruses and pathogenic bacteria. Moreover, advanced nanotechnologies have also been integrated into these biosensors to improve their detection stability, sensitivity, and accuracy. In this review, the recent advance in CRISPR/Cas systems-based nano/biosensors and their applications in the detection of infectious viruses and pathogenic bacteria are comprehensively reviewed. First of all, the categories and working principles of CRISPR/Cas systems for establishing the nano/biosensors are simply introduced. Then, the design and construction of CRISPR/Cas systems-based nano/biosensors are comprehensively discussed. In the end, attentions are focused on the applications of CRISPR/Cas systems-based nano/biosensors in the detection of infectious viruses and pathogenic bacteria. Impressively, the remaining opportunities and challenges for the further design and development of CRISPR/Cas system-based nano/biosensors and their promising applications are proposed.

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Section: Colorimetric Bio/nanosensingmentioning

confidence: 99%

CRISPR/Cas Systems‐Inspired Nano/Biosensors for Detecting Infectious Viruses and Pathogenic Bacteria

et al. 2022

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“…MOFs embedded biomolecular, especially the composites of MOFs and enzymes, are widely used in sensing and detection due to their diverse structures and functions. [63,71] Their applications in sensing basically involve colorimetric sensing, fluorescence sensing, chemiluminescence sensing and electrochemical sensing. [72] Biosensing is usually used for rapid and convenient detection in a complex environment, which requires high activity, stability and sensitivity of enzymes.…”

Section: Application Of Enzymes-immobilized Mofs Molecule/biomolecule...mentioning

confidence: 99%

Enzyme‐Immobilized Metal‐Organic Frameworks: From Preparation to Application

Wang

et al. 2022

Chemistry An Asian Journal

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As a class of widely used biocatalysts, enzymes possess advantages including high catalytic efficiency, strong specificity and mild reaction condition. However, most free enzymes have high requirements on the reaction environment and are easy to deactivate. Immobilization of enzymes on nanomaterial‐based substrates is a good way to solve this problem. Metal‐organic framework (MOFs), with ultra‐high specific surface area and adjustable porosity, can provide a large space to carry enzymes. And the tightly surrounded protective layer of MOFs can stabilize the enzyme structure to a great extent. In addition, the unique porous network structure enables selective mass transfer of substrates and facilitates catalytic processes. Therefore, these enzyme‐immobilized MOFs have been widely used in various research fields, such as molecule/biomolecule sensing and imaging, disease treatment, energy and environment protection. In this review, the preparation strategies and applications of enzyme‐immobilized MOFs are illustrated and the prospects and current challenges are discussed.

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“…Several authors have published reviews that discuss biocatalysts composed of enzymes and MOFs, addressing the most common methods of synthesis and immobilization of these composites, as well as their several applications [ 86 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 ]. In this scenario, this review intends to update the discussion of MOFs as highly relevant materials for a wide range of applications, as well as to discuss their roles and mechanisms of action as supports for enzymatic immobilization, and the different combinations for the formation of enzyme–MOF composites to diverse ends, such as catalysis, medicine, and in biosensor manufacturing, among others.…”

Section: Introductionmentioning

confidence: 99%

The Chemistry and Applications of Metal–Organic Frameworks (MOFs) as Industrial Enzyme Immobilization Systems

et al. 2022

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Enzymatic biocatalysis is a sustainable technology. Enzymes are versatile and highly efficient biocatalysts, and have been widely employed due to their biodegradable nature. However, because the three-dimensional structure of these enzymes is predominantly maintained by weaker non-covalent interactions, external conditions, such as temperature and pH variations, as well as the presence of chemical compounds, can modify or even neutralize their biological activity. The enablement of this category of processes is the result of the several advances in the areas of molecular biology and biotechnology achieved over the past two decades. In this scenario, metal–organic frameworks (MOFs) are highlighted as efficient supports for enzyme immobilization. They can be used to ‘house’ a specific enzyme, providing it with protection from environmental influences. This review discusses MOFs as structures; emphasizes their synthesis strategies, properties, and applications; explores the existing methods of using immobilization processes of various enzymes; and lists their possible chemical modifications and combinations with other compounds to formulate the ideal supports for a given application.

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Recent advances in enzyme immobilization based on novel porous framework materials and its applications in biosensing

Cited by 150 publications

References 181 publications

CRISPR/Cas Systems‐Inspired Nano/Biosensors for Detecting Infectious Viruses and Pathogenic Bacteria

CRISPR/Cas Systems‐Inspired Nano/Biosensors for Detecting Infectious Viruses and Pathogenic Bacteria

Enzyme‐Immobilized Metal‐Organic Frameworks: From Preparation to Application

The Chemistry and Applications of Metal–Organic Frameworks (MOFs) as Industrial Enzyme Immobilization Systems

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