Recent Advances in Electrical Impedance Sensing Technology for Single-Cell Analysis

Zhang, Zhao; Huang, Xiaowen; Liu, Ke; Lan, Tiancong; Wang, Zixin; Zhu, Zhen

doi:10.3390/bios11110470

Cited by 27 publications

(17 citation statements)

References 151 publications

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“…This narrow microchannel allowed for a more accurate impedance measurement of human polymorphonuclear leukocytes and teleost fish red blood cells. Subsequently, the electrical and equivalent circuit models of single cells were established [3,4].…”

Section: Introductionmentioning

confidence: 99%

A Review on Microfluidics-Based Impedance Biosensors

et al. 2023

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Electrical impedance biosensors are powerful and continuously being developed for various biological sensing applications. In this line, the sensitivity of impedance biosensors embedded with microfluidic technologies, such as sheath flow focusing, dielectrophoretic focusing, and interdigitated electrode arrays, can still be greatly improved. In particular, reagent consumption reduction and analysis time-shortening features can highly increase the analytical capabilities of such biosensors. Moreover, the reliability and efficiency of analyses are benefited by microfluidics-enabled automation. Through the use of mature microfluidic technology, complicated biological processes can be shrunk and integrated into a single microfluidic system (e.g., lab-on-a-chip or micro-total analysis systems). By incorporating electrical impedance biosensors, hand-held and bench-top microfluidic systems can be easily developed and operated by personnel without professional training. Furthermore, the impedance spectrum provides broad information regarding cell size, membrane capacitance, cytoplasmic conductivity, and cytoplasmic permittivity without the need for fluorescent labeling, magnetic modifications, or other cellular treatments. In this review article, a comprehensive summary of microfluidics-based impedance biosensors is presented. The structure of this article is based on the different substrate material categorizations. Moreover, the development trend of microfluidics-based impedance biosensors is discussed, along with difficulties and challenges that may be encountered in the future.

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Section: Introductionmentioning

confidence: 99%

A Review on Microfluidics-Based Impedance Biosensors

et al. 2023

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“…Flow cytometry enables the improved understanding of cell functions, the measurement of biophysical information on individual cells, and the characterization of cell heterogeneity in a large population, − which provides important tools for fundamental biological research and clinical diagnosis . As a novel method for precise cell manipulation and detection, microfluidics provides new insights for realizing the high-throughput cell focusing, counting, and identification functions of conventional flow cytometers due to the offered advantages of low device cost, simple operation, low sample consumption, and parallel processing capacity. , In a microfluidic flow cytometer, the fluorescent signals, optical images, , and impedance changes , of cells could be measured in a high-throughput and continuous-flow manner when the cells flow through the detection region. The key prerequisite for enabling the accurate cytometry detection is the three-dimensional (3D) focusing of cells, which makes the cells pass through the detection region one by one at fixed cross-sectional positions and avoids the detection errors caused by the position variation and the simultaneous existence of multiplex cells in the detection region …”

Section: Introductionmentioning

confidence: 99%

Controllable Size-Independent Three-Dimensional Inertial Focusing in High-Aspect-Ratio Asymmetric Serpentine Microchannels

Zhou

Zhu

et al. 2022

Anal. Chem.

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High-throughput three-dimensional (3D) focusing of cells is the key prerequisite for enabling accurate microfluidic cell detection and analysis. In this work, we develop a high-aspectratio asymmetric serpentine (HARAS) microchannel for single-line inertial focusing of particles and cells at the 3D center of the channel. The mechanism of 3D focusing is explored by numerical simulation, and the focusing performances of differently sized particles are characterized experimentally at different flow rates. The results demonstrate the outstanding 3D single-line focusing capability of our HARAS microchannel. In addition, the phenomena of size-independent and position-controllable focusing over wide flow rates are observed. Finally, the applicability of our HARAS microchannel for processing real biological cells is validated by the 3D single-line focusing of A549 cells and MCF-7 cells. Our work overcomes the issue of off-centered focusing of most previous works and provides new insights into the 3D focusing in inertial microfluidics. The proposed HARAS microchannel is extremely easy for mass production and may provide a stable, high-throughput, and position-controllable scheme for subsequent single-cell detection and analysis.

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“…We used a trapezoidal spiral channel for the separation of CTCs and background RBCs as the first stage at the top, followed by a two-stage square serpentine channel for further removal of RBCs and purification of the target sample solution in the middle and at the bottom, as shown in Figure 1. Through the flow rate reduction with the integration of spiral and serpentine channels, a variety of detection methods such as impedance detection [25] and imaging [26,27] can be applied at the channel collection outlet. Therefore, the multistage sorting chip can realize high flow rate input and low flow rate output and meet the requirements of medical diagnosis for throughput and detection.…”

Section: Introductionmentioning

confidence: 99%

3D-Stacked Multistage Inertial Microfluidic Chip for High-Throughput Enrichment of Circulating Tumor Cells

Huang

Sun

et al. 2022

Cyborg Bionic Syst

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Whether for cancer diagnosis or single-cell analysis, it remains a major challenge to isolate the target sample cells from a large background cell for high-efficiency downstream detection and analysis in an integrated chip. Therefore, in this paper, we propose a 3D-stacked multistage inertial microfluidic sorting chip for high-throughput enrichment of circulating tumor cells (CTCs) and convenient downstream analysis. In this chip, the first stage is a spiral channel with a trapezoidal cross-section, which has better separation performance than a spiral channel with a rectangular cross-section. The second and third stages adopt symmetrical square serpentine channels with different rectangular cross-section widths for further separation and enrichment of sample cells reducing the outlet flow rate for easier downstream detection and analysis. The multistage channel can separate 5 μ m and 15 μ m particles with a separation efficiency of 92.37% and purity of 98.10% at a high inlet flow rate of 1.3 mL/min. Meanwhile, it can separate tumor cells (SW480, A549, and Caki-1) from massive red blood cells (RBCs) with a separation efficiency of >80%, separation purity of >90%, and a concentration fold of ~20. The proposed work is aimed at providing a high-throughput sample processing system that can be easily integrated with flowing sample detection methods for rapid CTC analysis.

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Recent Advances in Electrical Impedance Sensing Technology for Single-Cell Analysis

Cited by 27 publications

References 151 publications

A Review on Microfluidics-Based Impedance Biosensors

A Review on Microfluidics-Based Impedance Biosensors

Controllable Size-Independent Three-Dimensional Inertial Focusing in High-Aspect-Ratio Asymmetric Serpentine Microchannels

3D-Stacked Multistage Inertial Microfluidic Chip for High-Throughput Enrichment of Circulating Tumor Cells

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