The study aimed to investigate and compare characteristics of Bombyx mori silk film coated with mimicked re-self assembly collagen type I under various conditions concentration [0.25,0.5,1.0,2.0 mg/ml]. The thin films consist of collagen fibrils and result from the self-assembly of collagen. The morphological characteristics observed by scanning electron microscopy (SEM) showed that when increased in collagen concentration, collagen became increase the size of the fibrils. The structure and thermal behavior all kind of silk coated with collagen were determined by FT-IR and DSC instrument. FT-IR spectra showed both spectra of silk and collagens are assigned to β-sheet conformation of silk fibroin and collagen type I. The stability thermal peak shifted to slightly higher temperature by increasing collagen concentration content. Mechanical properties of silk film coated collagen demonstrated that coating collagen at concentration 1-2 mg/ml resulted in modification of mechanical features, with increased maximum load, stress, strain, and Young's modulus .