Recent Advances in Droplet Microfluidics

Ding, Yun; Howes, Philip D.; deMello, Andrew J.

doi:10.1021/acs.analchem.9b05047

Cited by 224 publications

(172 citation statements)

References 220 publications

(326 reference statements)

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“…The behavior of the droplets for the different applied potentials shown in Fig. 3 can be described qualitatively by the Lippmann-Young equation (eqn (1) in the ESI †). 61,62 This describes the change of the contact angle θ and the wetting properties cos θ depending on the applied electrical potential.…”

Section: Resultsmentioning

confidence: 97%

“…In particular, droplet microfluidics, where femto-to microliter samples are encapsulated by an immiscible carrier fluid, is used for various applications including high-throughput screenings in chemical synthesis or bioassays. [1][2][3][4][5][6] An important aspect to make droplet microfluidics a valuable tool for versatile applications is the choice of a suitable detection technology. For this purpose, mass spectrometry is becoming increasingly popular as it is nearly universally usable and does not require labeling steps.…”

Section: Introductionmentioning

confidence: 99%

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How electrospray potentials can disrupt droplet microfluidics and how to prevent this

et al. 2020

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Section: Resultsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

How electrospray potentials can disrupt droplet microfluidics and how to prevent this

et al. 2020

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“…It is thus found that the maximum strain εmax = [4. [5][6] at c = 0.1c * , while εmax = [1][2] at c =10c * . This contrast supports the claim of incomplete stretch in the semi-dilute solutions.…”

Section: Capillary-driven Regimementioning

confidence: 99%

“…Droplet microfluidics has been one of the fastest growing areas of microfluidics in the past two decades, with far-reaching applications [1][2][3][4][5][6]. Microdroplets provide isolated micro-scale compartments for versatile functions in chemical, biological, medical, and material sciences [1][2][3][4][7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Breakup Dynamics of Semi-dilute Polymer Solutions in a Microfluidic Flow-focusing Device

Xue

Chen

et al. 2020

Micromachines

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Droplet microfluidics involving non-Newtonian fluids is of great importance in both fundamental mechanisms and practical applications. In the present study, breakup dynamics in droplet generation of semi-dilute polymer solutions in a microfluidic flow-focusing device were experimentally investigated. We found that the filament thinning experiences a transition from a flow-driven to a capillary-driven regime, analogous to that of purely elastic fluids, while the highly elevated viscosity and complex network structures in the semi-dilute polymer solutions induce the breakup stages with a smaller power-law exponent and extensional relaxation time. It is elucidated that the elevated viscosity of the semi-dilute solution decelerates filament thinning in the flow-driven regime and the incomplete stretch of polymer molecules results in the smaller extensional relaxation time in the capillary-driven regime. These results extend the understanding of breakup dynamics in droplet generation of non-Newtonian fluids and provide guidance for microfluidic synthesis applications involving dense polymeric fluids.

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“…[ 70 ] Therefore, the microfluidics technology has incomparable superiority in preparing multilayer architectures and facilitates the standardization and parallelization of artificial compartments. [ 71 ]…”

Section: Compartmentmentioning

confidence: 99%

Emerging Advances of Cell‐Free Systems toward Artificial Cells

et al. 2020

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models have attracted considerable attentions in order to unravel the inherent complexity. Although engineering modern cells has achieved some progress, [2] the arbitrary elimination of genomic information could probably remove some distinguished characteristics (top-down strategy). Building chemical systems that resemble the structure and behaviors of living cells by stepwise integration of pivotal components is also conceivable (bottom-up strategy). [3] These synthetic systems with biomimetic cellularity provide conceptual pathway from inanimate matter to artificial life, which are often known as artificial cells. [4] Due to the lack of full comprehension about the molecular mechanism of living cells, laborious reconstitution of complicated networks would be difficult and the disparities of protein behaviors in the artificial cells have been observed. Herein, we prefer to construct artificial cells by preserving certain endogenous machinery already existing in living cells. Such principle is conducible for maintaining essential features of living cells while leaving design space for synthetic biologists to study specific cellular behavior in bottom-up manner. [5] Cell-free system, also referred to as in vitro transcription and translation system is a universal toolbox to study the organisms of cell biology. [6] Cell-free extracts inherit most of the endogenous machinery including functional macromolecules as well as metabolic networks and the addition of desired resources could initiate coupled protein expressions. Indeed, the first appearance of cell-free systems could date back to 1961 when Nirenberg and Matthaei deciphered the genetic codes. [7] Featured by the accessible utilization of molecular machinery, cellfree systems could circumvent the frangibility and membrane barrier of biological cells. The transformative development of protein synthesis using recombinant elements (PURE) system with well-defined components greatly enhances the systemic manipulation and controllability. [8] In principle, the quintessence behind cell-free system is the central dogma that reflects the flow of genetic information, from plasmid DNA to messenger RNA (mRNA), to eventually make functional proteins. As such, artificial cells encapsulated with cell-free system could serve as the valuable candidate to construct numerous biological processes. [9] The powerful ability of protein expressions, which is deprived in other synthetic systems, offers advantageous scenario for enzymatic processes that are also guided by flexible and programmable regulations of gene circuit. [10] The execution of larger genetic programs could further permit the construction of complex biochemical systems. [11] More importantly, Artificial cells that mimic the architectural and functional characteristics of living cells not only shed light on the physical principle of life but also facilitate development in the areas such as cell engineering and biomedicine. Cell-free systems carry out the central dogma that refers to the transcription and translation ...

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Recent Advances in Droplet Microfluidics

Cited by 224 publications

References 220 publications

How electrospray potentials can disrupt droplet microfluidics and how to prevent this

How electrospray potentials can disrupt droplet microfluidics and how to prevent this

Breakup Dynamics of Semi-dilute Polymer Solutions in a Microfluidic Flow-focusing Device

Emerging Advances of Cell‐Free Systems toward Artificial Cells

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