Recent Advances in Design of Fluorescence-Based Assays for High-Throughput Screening

Abstract

Duan and Y. Liu. W. Zhong directed the review layout design, coordinated the writing efforts, and prepared the sections of Introduction and outlook and opportunities sections.

Rücktitelbild: Droplet Precise Self‐Splitting on Patterned Adhesive Surfaces for Simultaneous Multidetection (Angew. Chem. 26/2020)

“…Microdroplet-based microreactors require efficient separation of desired microdroplets and maintenance of their mutual independence during the whole reaction process. [30][31][32] Using the droplet self-splitting method, ac ontrollable number of microdroplets can be separated, and the arrangement of the microdroplets is designable through the high-adhesive pattern design. Meanwhile,t here is no matter exchange between the microdroplets in the droplet self-splitting process.C oncurrently,t he deposited microdroplets are fully isolated by the physical barrier of the low-adhesive stripes.…”

Spherical Gold Nanoparticles (AuNPs) Formation by Selected Intracellular Protein Fraction of Fungi

“…conditions of 25 ml P2 dosage (protein concentration of 0.87 mg/ml), pH=4, 30 ℃ and 0.5 mM AuCl4 -, the recovery of AuNPs by 10-30 kDa fraction rose to 94.64%, Size range of them changed from 8-30 nm to 6-25 nm, and the morphology of nanoscale particles was well controlled (changed from 87% to 93.5%, spherical shapes)(Figure 4). Shape and size control of AuNPs are useful in a wide range of applications, likes gold nanoclusters (smaller than 2 nm) can be used as fluorescent probes[2] and networked gold nanowires showed higher activity of catalytic properties[25]. Our study successfully obtained 10-30 kDa fraction of IPE from P. sanguineus, more monodisperse AuNPs were synthesized and 93.5% of AuNPs were spherical shapes, which provided the basis for improving the performance of AuNPs.…”

“…Gold nanoparticles (AuNPs) are the tiny particles of gold with a diameter of 1-100 nm. It possesses distinct physicochemical properties such as high electron density, dielectric properties, and good biocompatibility, gaining interest in technological applications such as sensors [1,2], electronic devices [3], bioimaging [4,5], and catalysis [6,7]. It is well known that the size and shape of AuNPs play a vital role in their performance and application, giving those remarkable electrical and optical properties and meeting the application of practical technology.…”

“…could be assigned to Au (0) and Au (I) respectively. The small amount of Au (I) (5%) presenting on the surface of the AuNPs helped to stabilize the particles electrostatically against aggregation in solution, also indicating the formation of AuNPs through intermediate Au (I) species[2]. The C1s core level centered at 284.6 eV, 286.5 eV and 288.9 eV corresponded to C-C bond, C-N bond, and the carbonyl bond respectively and the peak at 285.4 eV was caused by an indefinite carbon atom (Figure 6B).…”

Hairy Fluorescent Nanospheres Based on Polyelectrolyte Brush for Highly Sensitive Determination of Cu(II)

“…Heavy metal ion determination and removal are becoming more and more important. Copper (Cu(II)), a crucial cofactor for multiple enzymes, plays an critical role in bone formation together with certain proteins [1,2], but it is considered a pollutant when the concentration is over a critical level in the environment. It is easy for Cu(II) to form a complex that can change the molecular structure of a protein and break hydrogen bonds.…”