Recent advances in cell imaging and cytotoxicity of intracellular stimuli-responsive nanomaterials

Zhang, Wenbo; Gao, Changyou

doi:10.1007/s11434-015-0952-3

Cited by 27 publications

(11 citation statements)

References 55 publications

(78 reference statements)

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“…Precise detection of enzyme activity and its location within living cells provides a vital opportunity to unravel enzyme function in the biological process, which is essential for the diagnosis and treatment of diseases. , A myriad of analytical methods capable of detecting different enzyme activities have been actively reported. − Among them, fluorescence imaging with a fluorogenic probe is particularly attractive as its fluorescence is selectively activated by a target enzyme, whose catalytic ability could trigger continuous turnover of many molecules of probe. This could provide low background but signal amplification for sensitive and real-time measurement of various enzymes in living cells, such as β-galactosidase, − matrix metalloproteinase (MMP), − caspases, − cyclooxygenase-2 (COX-2), , and fibroblast activation protein-α (FAPα) …”

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confidence: 99%

Lysosome-Targeting Fluorogenic Probe for Cathepsin B Imaging in Living Cells

Wang

Feng

et al. 2016

Anal. Chem.

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Cathepsin B (CTB) is a lysosomal protease which has been recognized as a promising biomarker for many malignant tumors, and accurate detection of its activity is important in early diagnosis of cancers and predicting metastasis. Herein, we reported a lysosome-targeting fluorogenic small-molecule probe for fluorescence imaging of lysosomal CTB in living cancer cells by incorporating a CTB-recognitive peptide substrate Cbz-Lys-Lys-p-aminobenzyl alcohol (Cbz-Lys-Lys-PABA) and a lysosome locating group morpholine. We demonstrated that the probe could be efficiently activated by CTB to generate ∼73-fold enhancement in fluorescence under acidic lysosomal environment (pH 4.5-6.0), allowing for high sensitivity and specificity to detect CTB. Fluorescence imaging results showed selective accumulation and fluorescence turn-on in the lysosomes of cancer cells, which were capable of reporting on lysosomal CTB activity in cancer cells and normal tissue cells. This study highlights the potential of using a lysosome-targeting group to design a sensitive and specific fluorogenic probe for fluorescence imaging of lysosomal CTB in living cells.

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confidence: 99%

Lysosome-Targeting Fluorogenic Probe for Cathepsin B Imaging in Living Cells

Wang

Feng

et al. 2016

Anal. Chem.

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“…It is characterized by high sensitivity, is free of radioactive irradiation, and has the capacity for real-time imaging to obtain quantitative information about molecular targets [9]. Activatable fluorescence imaging probes that amplify the fluorescent signal upon interaction with a certain enzyme have several advantages for in vivo applications, including low background signal and high specificity to detect enzyme activity [10]. Moreover, activatable fluorescence imaging probes can also detect and monitor enzyme activity in real time.…”

Section: Activatable Optical Imaging Probesmentioning

confidence: 99%

Molecular imaging of enzyme activity in vivo using activatable probes

Yan

2016

Science Bulletin

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“…3-Amino-phenylboronic acid was used as the anchoring ligand to bind the b-lactamase specifically. N-isopropylacrylamide, as the thermoresponsive monomer, played a role on controllable capture and release towards blactamase [9]. It also should be noted that the target b- lactamase was captured by using the multiple interaction, ''recognition sites'' shape complementary and boronic acid affinity.…”

Section: Research Highlightmentioning

confidence: 99%