Recent advances in capillary electrophoresis separation of monosaccharides, oligosaccharides, and polysaccharides

Mantovani, Veronica; Galeotti, Fabio; Maccari, Francesca; Volpi, Nicola

doi:10.1002/elps.201700290

Cited by 98 publications

(49 citation statements)

References 107 publications

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“…Despite CE enabling high-resolution separation of heterogeneous mixtures of oligosaccharides [ 72 ], only a few works report CE analysis of AOS. The recent developments in CE techniques for carbohydrate analysis has previously been reviewed [ 73 ]. Polysaccharide analysis using carbohydrate gel electrophoresis (PACE) is a common separation technique used to separate oligosaccharides from natural products [ 74 ].…”

Section: Chromatographic Separation Of Oligosaccharidesmentioning

confidence: 99%

Oligosaccharides Derived from Red Seaweed: Production, Properties, and Potential Health and Cosmetic Applications

et al. 2018

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Because of their potential use as functional ingredients in human nutrition, oligosaccharides derived from natural sources are receiving paramount consideration. Red seaweed, a proven rich source of agar and carrageenan, is one of the most abundantly present sources of such oligosaccharides. Agaro-oligosaccharides (AOS) and carrageenan-oligosaccharides (COS) are produced from agar and carrageenan, respectively, through chemical and enzymatic hydrolyses. Enzymatic hydrolysis of agar and carrageenan into oligosaccharides is preferred in industrial production because of certain problems associated with chemical hydrolysis, including the release of high amounts of monosaccharides and undesirable toxic products, such as furfural. AOS and COS possess many biological activities, including prebiotic, immuno-modulatory, anti-oxidant, and anti-tumor activities. These activities are related to their chemical structure, molecular weight, degree of polymerization, and the flexibility of the glycosidic linkages. Therefore, the structure–function relationship and the mechanisms occurring during the specific biological applications of AOS and COS are discussed herein. Moreover, the chromatographic separation, purification, and characterization of AOS and COS are also part of this review. This piece of writing strives to create a new perspective on the potential applications of AOS and COS in the functional food and pharmaceutical industry.

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Section: Chromatographic Separation Of Oligosaccharidesmentioning

confidence: 99%

Oligosaccharides Derived from Red Seaweed: Production, Properties, and Potential Health and Cosmetic Applications

et al. 2018

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“…Capillary electrophoresis (CE) and gas chromatography (GC) are techniques with high sensitivity and resolution used for quantification of HMOs. However, these techniques often require derivatization, complicating the sample preparation (Balogh et al., ; Galeotti et al., ; Mantovani, Galeotti, Maccari, & Volpi, ).…”

Section: Introductionmentioning

confidence: 99%

Quantifying the human milk oligosaccharides 2’‐fucosyllactose and 3‐fucosyllactose in different food applications by high‐performance liquid chromatography with refractive index detection

Christensen

Skov

Lendal

et al. 2020

Journal of Food Science

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In recent years, production of biosynthesized human milk oligosaccharides (HMOs) has become scalable to industrial standards. As a result, infant formula fortified with 2'-fucosyllactose (2'-FL), the most abundant HMO in human breast milk, is now commercially available. 2'-FL and 3-fucosyllactose (3-FL), another abundant HMO, are thought to be beneficial for infant health and development. Products containing HMOs are projected to expand in the future, showing the need for robust, easily applicable analytical methods for the quantitative assessment of HMOs in different food applications. We present here a validated high-performance liquid chromatography method for the quantification of 2'-FL and 3-FL in whole milk, infant formula, and cereal bars. The sample preparation was simple dispersion and extraction of the sample. The samples were analyzed by hydrophilic interaction liquid chromatography with refractive index detection and a runtime of 19 min. The method had a high degree of linearity (R 2 > 0.9995) in the range 0.2 to 12 mg/mL. The recovery for 2'-FL was 88% to 105% and for 3-FL 94% to 112%. The limit of detection (LOD) for whole milk was 0.1 mg/mL for 2'-FL and 0.2 mg/mL for 3-FL. In infant formula and cereal bars, the LOD was 0.6 mg/g for both 2'-FL and 3-FL. To show the practical application of this method, it was successfully utilized in stability studies of 2'-FL and 3-FL in whole milk, UHT milk, and yoghurt. The method provides a means of simultaneous and robust quantification of 2'-FL and 3-FL in various food matrices with high accuracy and high reproducibility.Practical Application: 2'-Fucosyllactose (2'-FL) and 3-fucosyllactose (3-FL) are two of the most abundant human milk oligosaccharides (HMOs) present in human breast milk. We present a fast HPLC method for the robust quantification of these two compounds in infant formula, whole milk, UHT milk, cereal bars, and yoghurt. This method can easily be set up by food producers and researchers to analyze the dosage of 2'-FL and 3-FL in their product or perform shelf life studies in different food applications.

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“…Capillary electrophoresis (CE) is a powerful method for the separation and determination of various analytes such as amino acids, amines, steroids, nucleobases, nucleosides, nucleotides, peptides, proteins, and carbohydrates . Its separation efficiency is significantly higher compared to high performance liquid chromatography (HPLC).…”

Section: Introductionmentioning

confidence: 99%

Multifunction covalent coatings for separation of amino acids, biogenic amines, steroid hormones, and ketoprofen enantiomers by capillary electrophoresis and capillary electrochromatography

Кравченко

Колобова

Карцова

2020

Separation Science Plus

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In this study, the preparation process of multifunctional coating based on imidazole and β-cyclodextrin has been developed. The synthesis includes several steps: activation of fused silica capillary walls via alkali, then process of silylation and finally functionalization with imidazole and β-cyclodextrin derivative. The latter step was carried over 22 h at 100 • C at 750 mg/mL concentration of p-toluenesulfonyl-β-cyclodextrin.It was established that the coatings thus obtained are stable within pH 2-7 range but decompose in alkaline medium. The stationary phase developed in this study allows simultaneous separation of hydrophobic and hydrophilic analytes. Chiral separation of ketoprofen enantiomers was also achieved with the newly developed covalent coating.It was found that covalent coating based on imidazole and β-cyclodextrin exceeded the dynamic coatings of similar structure in terms of repeatability, analysis time and the limits of detection.

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Recent advances in capillary electrophoresis separation of monosaccharides, oligosaccharides, and polysaccharides

Cited by 98 publications

References 107 publications

Oligosaccharides Derived from Red Seaweed: Production, Properties, and Potential Health and Cosmetic Applications

Oligosaccharides Derived from Red Seaweed: Production, Properties, and Potential Health and Cosmetic Applications

Quantifying the human milk oligosaccharides 2’‐fucosyllactose and 3‐fucosyllactose in different food applications by high‐performance liquid chromatography with refractive index detection

Multifunction covalent coatings for separation of amino acids, biogenic amines, steroid hormones, and ketoprofen enantiomers by capillary electrophoresis and capillary electrochromatography

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