Recent advances in biotechnology for marine enzymes and molecules

Zhang, Jingyu; Jiang, Lan; Chen, Xiangyin; Lv, Kangjie; Basiony, Mostafa; Zhu, Guoliang; Karthik, Loganathan; Ouyang, Liming; Zhang, Lixin; Liu, Xueting

doi:10.1016/j.copbio.2021.05.009

Cited by 16 publications

(14 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, the α-amylase from C. baekdonensis B30 is unique among the genomes of Celeribacter, as the protein shared only 24.15% sequence identity compared to its reference homologue from C. halophilus ZXM137 T . α-Amylase is one of the oldest industrial enzymes and is used in various applications, including starch liquefaction and saccharification processes, food and beverage production, as an additive in textile detergents, paper processing, animal feed formulation, and bioethanol production [1,65,69]. Recently, α-amylase has also been applied as catalyst in the wastewater bioremediation, medicinal tablet formulation, and pharmaceutical biofilm inhibitory products [69].…”

Section: Analysis Of Cazymes and Mining Of Ghsmentioning

confidence: 99%

See 1 more Smart Citation

Genome Analysis of Celeribacter sp. PS-C1 Isolated from Sekinchan Beach in Selangor, Malaysia, Reveals Its β-Glucosidase and Licheninase Activities

et al. 2022

View full text Add to dashboard Cite

A halophilic marine bacterial strain, PS-C1, was isolated from Sekinchan beach in Selangor, Malaysia. The 16S rRNA gene sequence analysis indicated that strain PS-C1 was associated with the genus Celeribacter. To date, there have been no reports on enzymes from the genus Celeribacter. The present study reports on the cellular features of Celeribacter sp. PS-C1, its annotated genome sequence, and comparative genome analyses of Celeribacter glycoside hydrolase (GH) enzymes. The genome of strain PS-C1 has a size of 3.87 Mbp and a G+C content of 59.10%, and contains 3739 protein-coding genes. Detailed analysis using the Carbohydrate-Active enZYmes (CAZy) database revealed that Celeribacter genomes harboured at least 12 putative genes encoding industrially important GHs that are grouped as cellulases, β-glucanases, hemicellulases, and starch-degrading enzymes. Herein, the potential applications of these enzymes are discussed. Furthermore, the activities of two types of GHs (β-glucosidase and licheninase) in strain PS-C1 were demonstrated. These findings suggest that strain PS-C1 could be a reservoir of novel GH enzymes for lignocellulosic biomass degradation.

show abstract

Section: Analysis Of Cazymes and Mining Of Ghsmentioning

confidence: 99%

“…Marine environments are home to complex and diverse microorganisms that are yet to be discovered through appropriate microbial investigations. Currently, marinederived halophilic bacteria are being explored to harness their novel enzymes and bioactive compounds as substitutes for many industrial applications [1][2][3][4].…”

Section: Introductionmentioning

confidence: 99%

Genome Analysis of Celeribacter sp. PS-C1 Isolated from Sekinchan Beach in Selangor, Malaysia, Reveals Its β-Glucosidase and Licheninase Activities

et al. 2022

View full text Add to dashboard Cite

show abstract

“…I37C (Chiba et al, 2009), Rhodobacteraceae sp. (Novak et al, 2013a), Psychromonas such as barophilicity, salt tolerance, cold adaptability, hyperthermostability, chemoselectivity, stereoselectivity, and regioselectivity (Thippeswamy et al, 2021;Zhang J. et al, 2021). The marine environment is therefore expected to be an important source of novel enzymes.…”

Section: Isolation Sources and Classification Of 2-haloacid Dehalogenasesmentioning

confidence: 99%

Mini Review: Advances in 2-Haloacid Dehalogenases

et al. 2021

View full text Add to dashboard Cite

The 2-haloacid dehalogenases (EC 3.8.1.X) are industrially important enzymes that catalyze the cleavage of carbon–halogen bonds in 2-haloalkanoic acids, releasing halogen ions and producing corresponding 2-hydroxyl acids. These enzymes are of particular interest in environmental remediation and environmentally friendly synthesis of optically pure chiral compounds due to their ability to degrade a wide range of halogenated compounds with astonishing efficiency for enantiomer resolution. The 2-haloacid dehalogenases have been extensively studied with regard to their biochemical characterization, protein crystal structures, and catalytic mechanisms. This paper comprehensively reviews the source of isolation, classification, protein structures, reaction mechanisms, biochemical properties, and application of 2-haloacid dehalogenases; current trends and avenues for further development have also been included.

show abstract

“…Besides their ecological roles, marine microorganisms provide great opportunities for biotechnological exploitation as sources of enzymes (e.g., hydrolases, DNA polymerases, and oxidoreductases), bioactive molecules (e.g., antimicrobials and nutraceuticals), polymers (e.g., polysaccharides and polyhydroxyalkanoates), and biofuels (e.g., microalgae) which can be used in industries, medicine, and research [1,[3][4][5]. Among the numerous marine-derived products, enzymes feature prominently due to their continuously increasing demand on the global market.…”

Section: Introductionmentioning

confidence: 99%

Bioprospecting for Novel Bacterial Sources of Hydrolytic Enzymes and Antimicrobials in the Romanian Littoral Zone of the Black Sea

et al. 2022

View full text Add to dashboard Cite

Marine microorganisms have evolved a large variety of metabolites and biochemical processes, providing great opportunities for biotechnologies. In the search for new hydrolytic enzymes and antimicrobial compounds with enhanced characteristics, the current study explored the diversity of cultured and uncultured marine bacteria in Black Sea water from two locations along the Romanian coastline. Microbial cell density in the investigated samples varied between 65 and 12.7 × 103 CFU·mL−1. The total bacterial community identified by Illumina sequencing of 16S rRNA gene comprised 185 genera belonging to 46 classes, mainly Gammaproteobacteria, Alphaproteobacteria, Flavobacteriia, and 24 phyla. The 66 bacterial strains isolated on seawater-based culture media belonged to 33 genera and showed variable growth temperatures, growth rates, and salt tolerance. A great fraction of these strains, including Pseudoalteromonas and Flavobacterium species, produced extracellular proteases, lipases, and carbohydrases, while two strains belonging to the genera Aquimarina and Streptomyces exhibited antimicrobial activity against human pathogenic bacteria. This study led to a broader view on the diversity of microbial communities in the Black Sea, and provided new marine strains with hydrolytic and antimicrobial capabilities that may be exploited in industrial and pharmaceutical applications.

show abstract

Recent advances in biotechnology for marine enzymes and molecules

Cited by 16 publications

References 75 publications

Genome Analysis of Celeribacter sp. PS-C1 Isolated from Sekinchan Beach in Selangor, Malaysia, Reveals Its β-Glucosidase and Licheninase Activities

Genome Analysis of Celeribacter sp. PS-C1 Isolated from Sekinchan Beach in Selangor, Malaysia, Reveals Its β-Glucosidase and Licheninase Activities

Mini Review: Advances in 2-Haloacid Dehalogenases

Bioprospecting for Novel Bacterial Sources of Hydrolytic Enzymes and Antimicrobials in the Romanian Littoral Zone of the Black Sea

Contact Info

Product

Resources

About