Recent advances in bioprinting technologies for engineering hepatic tissue

Agarwal, Tarun; Banerjee, Dishary; Konwarh, Rocktotpal; Esworthy, Timothy; Kumari, Jaya; Onesto, Valentina; Das, Prativa; Lee, Bae Hoon; Wagener, Frank A. D. T. G.; Makvandi, Pooyan; Mattoli, Virgilio; Ghosh, Sudip Kumar; Maiti, Tapas K.; Zhang, Lijie Grace; Özbolat, İbrahim T.

doi:10.1016/j.msec.2021.112013

Cited by 35 publications

(16 citation statements)

References 236 publications

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“…Bioprinting has emerged as a powerful technique to deliver living cells embedded in a biomaterial in an organized pattern to build an intricate 3D structure layer by layer [ 1 , 2 ]. It offers advantages in terms of high repeatability, controllability, throughput, and positioning of multiple cells simultaneously [ 3 , 4 ]. However, proof-of-principle studies with 3D printing have been restricted to simple tissues such as skin and cardiac tissue [ 5 , 6 ], whereas heterogeneous and complex organs, such as the liver, are still challenging to engineer due to biomaterial limitations.…”

Section: Introductionmentioning

confidence: 99%

3D Bioprinting of Multi-Material Decellularized Liver Matrix Hydrogel at Physiological Temperatures

et al. 2022

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Bioprinting is an acclaimed technique that allows the scaling of 3D architectures in an organized pattern but suffers from a scarcity of appropriate bioinks. Decellularized extracellular matrix (dECM) from xenogeneic species has garnered support as a biomaterial to promote tissue-specific regeneration and repair. The prospect of developing dECM-based 3D artificial tissue is impeded by its inherent low mechanical properties. In recent years, 3D bioprinting of dECM-based bioinks modified with additional scaffolds has advanced the development of load-bearing constructs. However, previous attempts using dECM were limited to low-temperature bioprinting, which is not favorable for a longer print duration with cells. Here, we report the development of a multi-material decellularized liver matrix (dLM) bioink reinforced with gelatin and polyethylene glycol to improve rheology, extrudability, and mechanical stability. This shear-thinning bioink facilitated extrusion-based bioprinting at 37 °C with HepG2 cells into a 3D grid structure with a further enhancement for long-term applications by enzymatic crosslinking with mushroom tyrosinase. The heavily crosslinked structure showed a 16-fold increase in viscosity (2.73 Pa s−1) and a 32-fold increase in storage modulus from the non-crosslinked dLM while retaining high cell viability (85–93%) and liver-specific functions. Our results show that the cytocompatible crosslinking of dLM bioink at physiological temperatures has promising applications for extended 3D-printing procedures.

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Section: Introductionmentioning

confidence: 99%

3D Bioprinting of Multi-Material Decellularized Liver Matrix Hydrogel at Physiological Temperatures

et al. 2022

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“…is highly complex and dynamic, which is governed by multiple biochemical, biophysical, and environmental factors. [92][93][94][95][96] Exposure to infectious agents, especially pathogenic bacteria, is one such environmental factor that adversely affects the healing process and delays the recovery of damaged tissue. Agents with antibacterial properties, such as antibiotics and nanoparticles, have been used in tissue-engineered constructs (e.g., bandages, scaffolds, and hydrogels) in the form of coatings, Figure 9.…”

Section: Regenerative Medicinementioning

confidence: 99%

Antimicrobial Ionic Liquid‐Based Materials for Biomedical Applications

Nikfarjam

Ghomi

Agarwal

et al. 2021

Adv Funct Materials

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Excessive and unwarranted administration of antibiotics has invigorated the evolution of multidrug-resistant microbes. There is, therefore, an urgent need for advanced active compounds. Ionic liquids with short-lived ion-pair structures are highly tunable and have diverse applications. Apart from their unique physicochemical features, the newly discovered biological activities of ionic liquids have fascinated biochemists, microbiologists, and medical scientists. In particular, their antimicrobial properties have opened new vistas in overcoming the current challenges associated with combating antibioticresistant pathogens. Discussions regarding ionic liquid derivatives in monomeric and polymeric forms with antimicrobial activities are presented here. The antimicrobial mechanism of ionic liquids and parameters that affect their antimicrobial activities, such as chain length, cation/anion type, cation density, and polymerization, are considered. The potential applications of ionic liquids in the biomedical arena, including regenerative medicine, biosensing, and drug/biomolecule delivery, are presented to stimulate the scientific community to further improve the antimicrobial efficacy of ionic liquids.

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“…Inkjet-based bioprinting utilizes different droplet formation mechanisms; this involves the use of thermal, piezoelectric, acoustic, electromagnetic, or electrohydrodynamic forces to print tissues by successively dropping single droplets of the bioink onto a substrate ( Figure 1A ) ( Matsusaki et al, 2013 ; Cidonio et al, 2019 ; Ma et al, 2020 ). Inkjet-based bioprinting can rapidly fabricate high-resolution structures as it modulates the droplet size more precisely than the filaments generated by extrusion-based bioprinting ( Donderwinkel et al, 2017 ; Ma et al, 2020 ; Agarwal et al, 2021 ). Drops with diameters less than 50 μm have also been synthesized ( Matsusaki et al, 2013 ).…”

Section: Overview Of 3d Bioprintingmentioning

confidence: 99%

Review on Multicomponent Hydrogel Bioinks Based on Natural Biomaterials for Bioprinting 3D Liver Tissues

Kim

Lee

et al. 2022

Front. Bioeng. Biotechnol.

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Three-dimensional (3D)-printed in vitro tissue models have been used in various biomedical fields owing to numerous advantages such as enhancements in cell response and functionality. In liver tissue engineering, several studies have been reported using 3D-printed liver tissue models with improved cellular responses and functions in drug screening, liver disease, and liver regenerative medicine. However, the application of conventional single-component bioinks for the printing of 3D in vitro liver constructs remains problematic because of the complex structural and physiological characteristics of the liver. The use of multicomponent bioinks has become an attractive strategy for bioprinting 3D functional in vitro liver tissue models because of the various advantages of multicomponent bioinks, such as improved mechanical properties of the printed tissue construct and cell functionality. Therefore, it is essential to review various 3D bioprinting techniques and multicomponent hydrogel bioinks proposed for liver tissue engineering to suggest future directions for liver tissue engineering. Accordingly, we herein review multicomponent bioinks for 3D-bioprinted liver tissues. We first describe the fabrication methods capable of printing multicomponent bioinks and introduce considerations for bioprinting. We subsequently categorize and evaluate the materials typically utilized for multicomponent bioinks based on their characteristics. In addition, we also review recent studies for the application of multicomponent bioinks to fabricate in vitro liver tissue models. Finally, we discuss the limitations of current studies and emphasize aspects that must be resolved to enhance the future applicability of such bioinks.

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Recent advances in bioprinting technologies for engineering hepatic tissue

Cited by 35 publications

References 236 publications

3D Bioprinting of Multi-Material Decellularized Liver Matrix Hydrogel at Physiological Temperatures

3D Bioprinting of Multi-Material Decellularized Liver Matrix Hydrogel at Physiological Temperatures

Antimicrobial Ionic Liquid‐Based Materials for Biomedical Applications

Review on Multicomponent Hydrogel Bioinks Based on Natural Biomaterials for Bioprinting 3D Liver Tissues

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