Recent advances in bioprinting technologies for engineering different cartilage-based tissues

Agarwal, Tarun; Chiesa, Irene; Presutti, Dario; Irawan, Vincent; Vajanthri, Kiran Yellappa; Costantini, Marco; Nakagawa, Yasuhiro; Tan, Sheri-Ann; Makvandi, Pooyan; Zare, Ehsan Nazarzadeh; Sharifi, Esmaeel; Maria, Carmelo De; Ikoma, Toshiyuki; Maiti, Tapas K.

doi:10.1016/j.msec.2021.112005

Cited by 34 publications

(29 citation statements)

References 165 publications

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“…Traditional scaffold fabrication techniques such as foam processing, solution casting, and freeze-drying have limited control on the chemistry, macrostructure, and porosity of final products. Electrospinning and 3D bioprinting are two advanced manufacturing technologies for making desirable tissue engineering scaffolds [ 235 , 236 ]. Scaffolds prepared using these two techniques are hollow matrices that support cell structures and improve cell adhesion and proliferation due to their highly porous geometry which facilitate the transport of oxygen, nutrients, and biological wastes.…”

Section: Fabrication Methodsmentioning

confidence: 99%

Advanced Hydrogels for Cartilage Tissue Engineering: Recent Progress and Future Directions

et al. 2021

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Cartilage is a tension- and load-bearing tissue and has a limited capacity for intrinsic self-healing. While microfracture and arthroplasty are the conventional methods for cartilage repair, these methods are unable to completely heal the damaged tissue. The need to overcome the restrictions of these therapies for cartilage regeneration has expanded the field of cartilage tissue engineering (CTE), in which novel engineering and biological approaches are introduced to accelerate the development of new biomimetic cartilage to replace the injured tissue. Until now, a wide range of hydrogels and cell sources have been employed for CTE to either recapitulate microenvironmental cues during a new tissue growth or to compel the recovery of cartilaginous structures via manipulating biochemical and biomechanical properties of the original tissue. Towards modifying current cartilage treatments, advanced hydrogels have been designed and synthesized in recent years to improve network crosslinking and self-recovery of implanted scaffolds after damage in vivo. This review focused on the recent advances in CTE, especially self-healing hydrogels. The article firstly presents the cartilage tissue, its defects, and treatments. Subsequently, introduces CTE and summarizes the polymeric hydrogels and their advances. Furthermore, characterizations, the advantages, and disadvantages of advanced hydrogels such as multi-materials, IPNs, nanomaterials, and supramolecular are discussed. Afterward, the self-healing hydrogels in CTE, mechanisms, and the physical and chemical methods for the synthesis of such hydrogels for improving the reformation of CTE are introduced. The article then briefly describes the fabrication methods in CTE. Finally, this review presents a conclusion of prevalent challenges and future outlooks for self-healing hydrogels in CTE applications.

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Section: Fabrication Methodsmentioning

confidence: 99%

Advanced Hydrogels for Cartilage Tissue Engineering: Recent Progress and Future Directions

et al. 2021

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“…Limited material selection Corbel et al, 2011;Agarwal et al, 2021 PEGDA, poly(ethylene glycol) diacrylate; RNTK, lysine-functionalized rosette nanotubes.…”

Section: Recent Advancements 3d Printingmentioning

confidence: 99%

“…At present, there are many studies using FDM to produce a PCL framework and then combining hydrogels to fabricate composite meniscal scaffolds. However, FDM techniques suffer from poor surface quality and difficulties in combining biopolymers owing to their high extrusion temperatures ( Agarwal et al, 2021 ). The SLA technique is characterized by high resolution; however, a limited selection of photopolymers restricts its application in tissue engineering, and it has not yet been applied in meniscal tissue engineering ( Qu et al, 2021 ).…”

Section: Recent Advancementsmentioning

confidence: 99%

Meniscal Regenerative Scaffolds Based on Biopolymers and Polymers: Recent Status and Applications

Yang

et al. 2021

Front. Cell Dev. Biol.

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Knee menisci are structurally complex components that preserve appropriate biomechanics of the knee. Meniscal tissue is susceptible to injury and cannot heal spontaneously from most pathologies, especially considering the limited regenerative capacity of the inner avascular region. Conventional clinical treatments span from conservative therapy to meniscus implantation, all with limitations. There have been advances in meniscal tissue engineering and regenerative medicine in terms of potential combinations of polymeric biomaterials, endogenous cells and stimuli, resulting in innovative strategies. Recently, polymeric scaffolds have provided researchers with a powerful instrument to rationally support the requirements for meniscal tissue regeneration, ranging from an ideal architecture to biocompatibility and bioactivity. However, multiple challenges involving the anisotropic structure, sophisticated regenerative process, and challenging healing environment of the meniscus still create barriers to clinical application. Advances in scaffold manufacturing technology, temporal regulation of molecular signaling and investigation of host immunoresponses to scaffolds in tissue engineering provide alternative strategies, and studies have shed light on this field. Accordingly, this review aims to summarize the current polymers used to fabricate meniscal scaffolds and their applications in vivo and in vitro to evaluate their potential utility in meniscal tissue engineering. Recent progress on combinations of two or more types of polymers is described, with a focus on advanced strategies associated with technologies and immune compatibility and tunability. Finally, we discuss the current challenges and future prospects for regenerating injured meniscal tissues.

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“…The emergence of cartilage tissue engineering has led to a surge in biomaterial demand. The objective in designing biomaterials is to provide a good environment for cell growth and mechanically support regenerated tissue [6,7]. As a kind of biomaterial, hydrogel has been widely studied as an alternative to cartilage tissue due to its inherent water content, high hydrophilicity, and viscoelasticity similar to cartilage tissue [8].…”

Section: Introductionmentioning

confidence: 99%

Development and Evaluation of Gellan Gum/Silk Fibroin/Chondroitin Sulfate Ternary Injectable Hydrogel for Cartilage Tissue Engineering

et al. 2021

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Hydrogel is in the spotlight as a useful biomaterial in the field of drug delivery and tissue engineering due to its similar biological properties to a native extracellular matrix (ECM). Herein, we proposed a ternary hydrogel of gellan gum (GG), silk fibroin (SF), and chondroitin sulfate (CS) as a biomaterial for cartilage tissue engineering. The hydrogels were fabricated with a facile combination of the physical and chemical crosslinking method. The purpose of this study was to find the proper content of SF and GG for the ternary matrix and confirm the applicability of the hydrogel in vitro and in vivo. The chemical and mechanical properties were measured to confirm the suitability of the hydrogel for cartilage tissue engineering. The biocompatibility of the hydrogels was investigated by analyzing the cell morphology, adhesion, proliferation, migration, and growth of articular chondrocytes-laden hydrogels. The results showed that the higher proportion of GG enhanced the mechanical properties of the hydrogel but the groups with over 0.75% of GG exhibited gelling temperatures over 40 °C, which was a harsh condition for cell encapsulation. The 0.3% GG/3.7% SF/CS and 0.5% GG/3.5% SF/CS hydrogels were chosen for the in vitro study. The cells that were encapsulated in the hydrogels did not show any abnormalities and exhibited low cytotoxicity. The biochemical properties and gene expression of the encapsulated cells exhibited positive cell growth and expression of cartilage-specific ECM and genes in the 0.5% GG/3.5% SF/CS hydrogel. Overall, the study of the GG/SF/CS ternary hydrogel with an appropriate content showed that the combination of GG, SF, and CS can synergistically promote articular cartilage defect repair and has considerable potential for application as a biomaterial in cartilage tissue engineering.

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Recent advances in bioprinting technologies for engineering different cartilage-based tissues

Cited by 34 publications

References 165 publications

Advanced Hydrogels for Cartilage Tissue Engineering: Recent Progress and Future Directions

Advanced Hydrogels for Cartilage Tissue Engineering: Recent Progress and Future Directions

Meniscal Regenerative Scaffolds Based on Biopolymers and Polymers: Recent Status and Applications

Development and Evaluation of Gellan Gum/Silk Fibroin/Chondroitin Sulfate Ternary Injectable Hydrogel for Cartilage Tissue Engineering

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