Recent advances in biological sample preparation methods coupled with chromatography, spectrometry and electrochemistry analysis techniques

Niu, Zheyu; Zhang, Weiwei; Chen, Yu; Jun, Zhang; Wen, Yingying

doi:10.1016/j.trac.2018.02.005

Cited by 145 publications

(80 citation statements)

References 267 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The conventional LLE and SPE methods are widely used to analyze biological samples [76]. However, these sample pretreatment techniques are associated with drawbacks such as complicated operations, time-consuming, the requirement of relatively large amounts of sample and organic solvents, strong matrix effect (the interference and influence of matrix on analyte analysis) and the difficulty to automate.…”

Section: Common Pretreatment Methods In Plant Hormone Analysismentioning

confidence: 99%

Recent developments and emerging trends of mass spectrometric methods in plant hormone analysis: a review

et al. 2020

View full text Add to dashboard Cite

Plant hormones are naturally occurring small molecule compounds which are present at trace amounts in plant. They play a pivotal role in the regulation of plant growth. The biological activity of plant hormones depends on their concentrations in the plant, thus, accurate determination of plant hormone is paramount. However, the complex plant matrix, wide polarity range and low concentration of plant hormones are the main hindrances to effective analyses of plant hormone even when state-of-the-art analytical techniques are employed. These factors substantially influence the accuracy of analytical results. So far, significant progress has been realized in the analysis of plant hormones, particularly in sample pretreatment techniques and mass spectrometric methods. This review describes the classic extraction and modern microextraction techniques used to analyze plant hormone. Advancements in solid phase microextraction (SPME) methods have been driven by the ever-increasing requirement for dynamic and in vivo identification of the spatial distribution of plant hormones in real-life plant samples, which would contribute greatly to the burgeoning field of plant hormone investigation. In this review, we describe advances in various aspects of mass spectrometry methods. Many fragmentation patterns are analyzed to provide the theoretical basis for the establishment of a mass spectral database for the analysis of plant hormones. We hope to provide a technical guide for further discovery of new plant hormones. More than 140 research studies on plant hormone published in the past decade are reviewed, with a particular emphasis on the recent advances in mass spectrometry and sample pretreatment techniques in the analysis of plant hormone. The potential progress for further research in plant hormones analysis is also highlighted.

show abstract

Section: Common Pretreatment Methods In Plant Hormone Analysismentioning

confidence: 99%

Recent developments and emerging trends of mass spectrometric methods in plant hormone analysis: a review

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Recently, salivary diagnostics shows excellent promise as a convenient means for the early detection, prognosis, and monitoring post-therapy status, as saliva is 99% water and can be easily manipulated [ 12 ]. Screening biomarkers in the saliva is advantageous because the sample extraction is simple, noninvasive, stress-free, cost-effective, and precise.…”

Section: Analytesmentioning

confidence: 99%

Recent Advances in Portable Biosensors for Biomarker Detection in Body Fluids

2020

View full text Add to dashboard Cite

A recent development in portable biosensors allows rapid, accurate, and on-site detection of biomarkers, which helps to prevent disease spread by the control of sources. Less invasive sample collection is necessary to use portable biosensors in remote environments for accurate on-site diagnostics and testing. For non- or minimally invasive sampling, easily accessible body fluids, such as saliva, sweat, blood, or urine, have been utilized. It is also imperative to find accurate biomarkers to provide better clinical intervention and treatment at the onset of disease. At the same time, these reliable biomarkers can be utilized to monitor the progress of the disease. In this review, we summarize the most recent development of portable biosensors to detect various biomarkers accurately. In addition, we discuss ongoing issues and limitations of the existing systems and methods. Lastly, we present the key requirements of portable biosensors and discuss ideas for functional enhancements.

show abstract

“…Generally, these procedures are focused on removing interferents from the matrix, and on extracting/pre-concentrating target analytes [13]. The most common sample preparation techniques are conventional solid-phase extraction (SPE) and liquid-liquid extraction (LLE), which were proposed more than 50 years ago.…”

Section: Introductionmentioning

confidence: 99%

Multidimensional Liquid Chromatography Employing a Graphene Oxide Capillary Column as the First Dimension: Determination of Antidepressant and Antiepileptic Drugs in Urine

et al. 2020

View full text Add to dashboard Cite

Human mental disorders can be currently classified as one of the most relevant health topics. Including in this are depression and anxiety, which can affect us at any stage of life, causing economic and social problems. The treatments involve cognitive psychotherapy, and mainly the oral intake of pharmaceutical antidepressants. Therefore, the development of analytical methods for monitoring the levels of these drugs in biological fluids is critical. Considering the current demand for sensitive and automated analytical methods, the coupling between liquid chromatography and mass spectrometry, combined with suitable sample preparation, becomes a useful way to improve the analytical results even more. Herein we present an automated multidimensional method based on high-performance liquid chromatography-tandem mass spectrometry using a lab-made, graphene-based capillary extraction column connected to a C8 analytical column to determined five pharmaceutical drugs in urine. A method enhancement was performed by considering the chromatographic separation and the variables of the loading phase, loading time, loading flow, and injection volume. Under optimized conditions, the study reports good linearity with R2 > 0.98, and limits of detection in the range of 0.5–20 µg L−1. Afterward, the method was applied to the direct analysis of ten untreated urine samples, reporting traces of citalopram in one of them. The results suggest that the proposed approach could be a promising alternative that provides direct and fully automated analysis of pharmaceutical drugs in complex biological matrices.

show abstract

Recent advances in biological sample preparation methods coupled with chromatography, spectrometry and electrochemistry analysis techniques

Cited by 145 publications

References 267 publications

Recent developments and emerging trends of mass spectrometric methods in plant hormone analysis: a review

Recent developments and emerging trends of mass spectrometric methods in plant hormone analysis: a review

Recent Advances in Portable Biosensors for Biomarker Detection in Body Fluids

Multidimensional Liquid Chromatography Employing a Graphene Oxide Capillary Column as the First Dimension: Determination of Antidepressant and Antiepileptic Drugs in Urine

Contact Info

Product

Resources

About