Recent advances in bio-orthogonal and dynamic crosslinking of biomimetic hydrogels

Arkenberg, Matthew R.; Nguyen, Han; Lin, Chien‐Chi

doi:10.1039/d0tb01429j

Cited by 73 publications

(59 citation statements)

References 170 publications

(210 reference statements)

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“…Therefore, the design of dynamic hydrogels that could modulate the biomechanical and chemical properties over time (4D hydrogels) could recreate the TME changes over different disease stages. The introduction of post‐gelation modifications in hydrogels by click chemistry or enzymes [ 181 ] or the use of stimuli‐sensitive biomaterials [ 182 ] to obtain new dynamic 4D hydrogels is starting to be explored in the field of tissue engineering, and this knowledge could be applicable to the development of more biomimetic 3D in vitro models. Another aspect that should be explored is the source of Col I and the combination of different collagen types.…”

Section: Engineering the Tme Using Protein‐based Hydrogelsmentioning

confidence: 99%

Proteinaceous Hydrogels for Bioengineering Advanced 3D Tumor Models

et al. 2021

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The establishment of tumor microenvironment using biomimetic in vitro models that recapitulate key tumor hallmarks including the tumor supporting extracellular matrix (ECM) is in high demand for accelerating the discovery and preclinical validation of more effective anticancer therapeutics. To date, ECM‐mimetic hydrogels have been widely explored for 3D in vitro disease modeling owing to their bioactive properties that can be further adapted to the biochemical and biophysical properties of native tumors. Gathering on this momentum, herein the current landscape of intrinsically bioactive protein and peptide hydrogels that have been employed for 3D tumor modeling are discussed. Initially, the importance of recreating such microenvironment and the main considerations for generating ECM‐mimetic 3D hydrogel in vitro tumor models are showcased. A comprehensive discussion focusing protein, peptide, or hybrid ECM‐mimetic platforms employed for modeling cancer cells/stroma cross‐talk and for the preclinical evaluation of candidate anticancer therapies is also provided. Further development of tumor‐tunable, proteinaceous or peptide 3D microtesting platforms with microenvironment‐specific biophysical and biomolecular cues will contribute to better mimic the in vivo scenario, and improve the predictability of preclinical screening of generalized or personalized therapeutics.

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Section: Engineering the Tme Using Protein‐based Hydrogelsmentioning

confidence: 99%

Proteinaceous Hydrogels for Bioengineering Advanced 3D Tumor Models

et al. 2021

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“…Reaction partners in bioorthogonal reactions do not occur in nature or cross-react with the common functional groups present in biology. Bioorthogonal chemistries have been extensively reviewed elsewhere, and interested readers are directed to several excellent recent reviews [28,[95][96][97].…”

Section: Secondary Crosslinking For Improved Stabilitymentioning

confidence: 99%

Supramolecular Host–Guest Hydrogels for Corneal Regeneration

Madl

Myung

2021

Gels

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Over 6.2 million people worldwide suffer from moderate to severe vision loss due to corneal disease. While transplantation with allogenic donor tissue is sight-restoring for many patients with corneal blindness, this treatment modality is limited by long waiting lists and high rejection rates, particularly in patients with severe tissue damage and ocular surface pathologies. Hydrogel biomaterials represent a promising alternative to donor tissue for scalable, nonimmunogenic corneal reconstruction. However, implanted hydrogel materials require invasive surgeries and do not precisely conform to tissue defects, increasing the risk of patient discomfort, infection, and visual distortions. Moreover, most hydrogel crosslinking chemistries for the in situ formation of hydrogels exhibit off-target effects such as cross-reactivity with biological structures and/or result in extractable solutes that can have an impact on wound-healing and inflammation. To address the need for cytocompatible, minimally invasive, injectable tissue substitutes, host–guest interactions have emerged as an important crosslinking strategy. This review provides an overview of host–guest hydrogels as injectable therapeutics and highlights the potential application of host–guest interactions in the design of corneal stromal tissue substitutes.

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“…[213] Hydrogel fabrication routinely involves chemical modification of macromer precursors to suit the application of well-characterized chemical crosslinking strategies such as free-radical initiated photocrosslinking, Michael-type addition, Diels-Alder, and strain promoted azide-alkyne cycloaddition "click" reactions among others, which yield 3D hydrogels that are cytocompatible and possess tunable biophysical properties. [214] The most common materials for 3D cell culture are collagen and Matrigel, which have been widely used to evaluate glioma cell morphology, invasion, proliferation, and stemness. [39,[215][216][217][218][219][220][221][222] These studies elucidated the effects of matrix concentration, composition, pore size, secreted factors, and the effect that other cells have on glioma cell invasion.…”

Section: Hydrogel Scaffolds and In Vitro Platform Technologiesmentioning

confidence: 99%

Glycomaterials to Investigate the Functional Role of Aberrant Glycosylation in Glioblastoma

Tondepu

Karumbaiah

2021

Adv Healthcare Materials

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Glioblastoma (GBM) is a stage IV astrocytoma that carries a dismal survival rate of ≈10 months postdiagnosis and treatment. The highly invasive capacity of GBM and its ability to escape therapeutic challenges are key factors contributing to the poor overall survival rate. While current treatments aim to target the cancer cell itself, they fail to consider the significant role that the GBM tumor microenvironment (TME) plays in promoting tumor progression and therapeutic resistance. The GBM tumor glycocalyx and glycan-rich extracellular matrix (ECM), which are important constituents of the TME have received little attention as therapeutic targets. A wide array of aberrantly modified glycans in the GBM TME mediate tumor growth, invasion, therapeutic resistance, and immunosuppression. Here, an overview of the landscape of aberrant glycan modifications in GBM is provided, and the design and utility of 3D glycomaterials are discussed as a tool to evaluate glycan-mediated GBM progression and therapeutic efficacy. The development of alternative strategies to target glycans in the TME can potentially unveil broader mechanisms of restricting tumor growth and enhancing the efficacy of tumor-targeting therapeutics.

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Recent advances in bio-orthogonal and dynamic crosslinking of biomimetic hydrogels

Abstract: In recent years, dynamic, ‘click’ hydrogels have been applied in numerous biomedical applications. Owing to the mild, cytocompatible, and highly specific reaction kinetics, a multitude of orthogonal handles have been...

Cited by 73 publications

References 170 publications

Proteinaceous Hydrogels for Bioengineering Advanced 3D Tumor Models

Proteinaceous Hydrogels for Bioengineering Advanced 3D Tumor Models

Supramolecular Host–Guest Hydrogels for Corneal Regeneration

Glycomaterials to Investigate the Functional Role of Aberrant Glycosylation in Glioblastoma

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