A study on water quality status of Tekala River, Selangor, Malaysia was conducted at two sampling times. A total of six sampling stations were selected along the river representing the upstream (S1 and S2), the middle stream (S3 and S4) and the downstream (S5 and S6) of the Tekala River. In this study, in-situ and ex-situ analyses were conducted to determine the quality of Tekala River. Physical, chemical and biological parameters included biochemical oxygen demand (BOD), chemical oxygen demand (SOD), ammoniacal nitrogen, total suspended solids (TSS), dissolved oxygen (DO), pH, temperature, total dissolved solid (TDS), salinity, conductivity, Escherichia coli, coliform and macroinvertebrate. According to the result obtained from this study, Tekala River is classified under Class I and Class II based on water quality index and National water quality standard. Two-way ANOVA showed a significant difference between parameters (ammoniacal nitrogen, BOD, pH, temperature, conductivity, DO and TDS) of sampling station. Significant difference was found between ammoniacal nitrogen, pH and temperature with sampling time. The correlation test revealed that there is relationship between Escherichia coli with ammoniacal nitrogen, temperature and DO. There is also relationship found between coliform with pH and BOD.