Recent advances in aptasensors for mycotoxin detection: On the surface and in the colloid

Zhang, Nan; Liu, Boshi; Cui, Xialian; Li, Yutong; Tang, Jing; Wang, Haixia; Zhang, Di; Li, Zheng

doi:10.1016/j.talanta.2020.121729

Cited by 46 publications

(21 citation statements)

References 129 publications

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“…In general, methods allowing aptamer-target binding occurring free in solution, as the MST assay described herein, are the preferred ones because they remove any contribution from matrix binding responsible for reducing aptamer functionality. On the other hand, colorimetric assays, like the MBs one, are widely applied in quantitative detection of mycotoxins, especially in the real-sample applications [29,30,32].…”

Section: Experimental Binding Affinity Of Aptamers To Mycotoxinsmentioning

confidence: 99%

“…A further confirmation of these results comes from the numbers of applications reported in literature for these three families of aptamers. In particular, the most numerous applications to food samples contaminated with mycotoxins were described for OTA aptamers followed by AFB1 aptamers and to a lesser extent for FB1 aptamers [29,30,32], thus suggesting the major versatility of OTA and AFB1 aptamers compared to the other ones.…”

Section: Comparison Of In-silico and Experimental Binding Affinity Of Aptamers To Mycotoxinsmentioning

confidence: 99%

“…To date, these aptamers have been integrated into several applications for mycotoxin analysis, including colorimetric, electrochemical, electrochemiluminescence and fluorescent biosensors, enzyme-linked assays and affinity chromatography approaches. Extensive reviews have been recently published covering the recent developments of aptamer-based biosensors for the detection of mycotoxins [29][30][31][32].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

An In-Silico Pipeline for Rapid Screening of DNA Aptamers against Mycotoxins: The Case-Study of Fumonisin B1, Aflatoxin B1 and Ochratoxin A

et al. 2020

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Aptamers are single-stranded oligonucleotides selected by SELEX (Systematic Evolution of Ligands by EXponential Enrichment) able to discriminate target molecules with high affinity and specificity, even in the case of very closely related structures. Aptamers have been produced for several targets including small molecules like mycotoxins; however, the high affinity for their respective target molecules is a critical requirement. In the last decade, the screening through computational methods of aptamers for their affinity against specific targets has greatly increased and is becoming a commonly used procedure due to its convenience and low costs. This paper describes an in-silico approach for rapid screening of ten ssDNA aptamer sequences against fumonisin B1 (FB1, n = 3), aflatoxin B1 (AFB1, n = 2) and ochratoxin A (OTA, n = 5). Theoretical results were compared with those obtained by testing the same aptamers by fluorescent microscale thermophoresis and by magnetic beads assay for their binding affinity (KD) revealing a good agreement.

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Section: Experimental Binding Affinity Of Aptamers To Mycotoxinsmentioning

confidence: 99%

Section: Comparison Of In-silico and Experimental Binding Affinity Of Aptamers To Mycotoxinsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

An In-Silico Pipeline for Rapid Screening of DNA Aptamers against Mycotoxins: The Case-Study of Fumonisin B1, Aflatoxin B1 and Ochratoxin A

et al. 2020

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“…Colorimetric biosensors based on aptamers have demonstrated their sensitivity and selectivity, in addition to their effective potential for rapid onsite diagnosis without complicated instrumentation. However, the colorimetric aptasensor has some limitations such as the influence of color from samples; the time-consuming nature of the fabrication process [57,58]; the difficulty in employing it in multiple-target assays, which are highly demanded in clinical diagnosis [59]; and the small range of optimized pH solutions [60].…”

Section: Colorimetric Aptasensormentioning

confidence: 99%

Recent Advances in Aptamer Sensors

Shaban

Kim

2021

Sensors

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Recently, aptamers have attracted attention in the biosensing field as signal recognition elements because of their high binding affinity toward specific targets such as proteins, cells, small molecules, and even metal ions, antibodies for which are difficult to obtain. Aptamers are single oligonucleotides generated by in vitro selection mechanisms via the systematic evolution of ligand exponential enrichment (SELEX) process. In addition to their high binding affinity, aptamers can be easily functionalized and engineered, providing several signaling modes such as colorimetric, fluorometric, and electrochemical, in what are known as aptasensors. In this review, recent advances in aptasensors as powerful biosensor probes that could be used in different fields, including environmental monitoring, clinical diagnosis, and drug monitoring, are described. Advances in aptamer-based colorimetric, fluorometric, and electrochemical aptasensing with their advantages and disadvantages are summarized and critically discussed. Additionally, future prospects are pointed out to facilitate the development of aptasensor technology for different targets.

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“…Zhang et al [57] reviewed the newly released mycotoxin aptasensors, with the aim to provide indications concerning practical applications and tailored design of aptasensors for mycotoxins and other analytes.…”

Section: Toxinsmentioning

confidence: 99%

Electrochemical Biosensors in Food Safety: Challenges and Perspectives

Curulli

2021

Preprint

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Safety and quality are key issues for the food industry. Consequently, there is a growing demand to preserve the food chain and products against substances toxic, harmful to human health such as contaminants, allergens, toxins, or pathogens. For this reason, it is mandatory to develop highly sensitive, reliable, rapid, and cost-effective sensing systems/devices such as electrochemical sensors/biosensors. Generally, conventional techniques are limited by long time of analyses, expensive and complex procedures, and they require skilled personnel. Therefore, the development of performant electrochemical biosensors can significantly support the screening of food chain and products. Here, we report some of the recent developments in this area and analyze the contributions produced by electrochemical biosensors in the food screening and the challenges to address.

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Recent advances in aptasensors for mycotoxin detection: On the surface and in the colloid

Cited by 46 publications

References 129 publications

An In-Silico Pipeline for Rapid Screening of DNA Aptamers against Mycotoxins: The Case-Study of Fumonisin B1, Aflatoxin B1 and Ochratoxin A

An In-Silico Pipeline for Rapid Screening of DNA Aptamers against Mycotoxins: The Case-Study of Fumonisin B1, Aflatoxin B1 and Ochratoxin A

Recent Advances in Aptamer Sensors

Electrochemical Biosensors in Food Safety: Challenges and Perspectives

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