Recent Advances in Analytical Methods on Lipoprotein Subclasses: Calculation of Particle Numbers from Lipid Levels by Gel Permeation HPLC Using “Spherical Particle Model”

Okazaki, Mitsuyo; Yamashita, Shizuya

doi:10.5650/jos.ess16020

Cited by 65 publications

(89 citation statements)

References 99 publications

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“…Considering the diameters of LDL particles, medium, small, and very small LDLs (F9—F13) are consistent with the classification of small dense LDL particles [13]. Very large HDLs (F14 and F15), large HDL (F16), and medium HDL (F17) correspond to HDL2, and small HDL (F18) and very small HDL (F19 and F20) to HDL3 [15]. The proportion of the cholesterol level in each lipoprotein fraction to total cholesterol level (cholesterol proportion) was calculated using the following formula: Cholesterol proportion (%) = cholesterol level in each lipoprotein fraction (mg/dl) / total cholesterol level (mg/dl) ×100.…”

Section: Methodssupporting

confidence: 74%

“…Serum soluble α-Klotho (Klotho) and FGF23 levels were measured using an immunoassay kit (human soluble α-Klotho assay kit, Immuno-Biological Laboratories Co., Gunma, Japan; FGF-23 ELISA Kit, Kainosu laboratories Inc., Tokyo, Japan) at Skylight Biotech Inc, Akita, Japan. Lipoprotein fractions were analyzed by HPGPC as previously described [13–15]. HPGPC was carried out to simultaneously measure cholesterol levels and particle numbers in lipoprotein fractions.…”

Section: Methodsmentioning

confidence: 99%

“…To evaluate the roles of lipoproteins in CKD patients, measurements of not only lipoprotein cholesterol levels but also lipoprotein particle size are required. We previously established a method of high-performance gel permeation chromatography (HPGPC), with which we can separate lipoproteins into 20 fractions, and reported an association between lipid profiles and peripheral artery disease in peritoneal dialysis patients [13–15]. Therefore, the aims of this cohort study were to investigate (1) the lipid profiles at subclass levels in CKD patients, (2) the relationships between lipid profiles and CKD progression, and (3) the relationships between lipid profiles, ankle-brachial index (ABI) as a marker of atherosclerosis in the peripheral artery, and (4) levels of the CKD-MBD-related markers (Klotho and FGF23) using HPGPC.…”

Section: Introductionmentioning

confidence: 99%

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Association of High-Density Lipoprotein Subclasses with Chronic Kidney Disease Progression, Atherosclerosis, and Klotho

Kanda

Okazaki

et al. 2016

PLoS ONE

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BackgroundAtherosclerosis is often a complication of chronic kidney disease (CKD) because of dyslipidemia and CKD-mineral and bone disorder. High-density lipoproteins (HDLs) are grouped into various subclasses composed of multiple proteins and lipids, and their transformation is altered in CKD. We investigated the roles of lipoprotein subclasses in CKD progression, and atherosclerosis, and the relationships with Klotho and fibroblast growth factor (FGF) 23.MethodsSeventy-one CKD patients were enrolled in this prospective cohort study in Japan. The proportions of cholesterol level to total cholesterol level (cholesterol proportion) and lipoprotein particle numbers in 20 lipoprotein fractions were measured by a newly developed high-performance gel permeation chromatography.ResultsDiabetic nephropathy was observed in 23.9% of the patients. The mean age was 75.0 years and estimated glomerular filtration rate (eGFR) was 17.2 ml/min./1.73m2. The lipoprotein particle numbers in small HDLs were higher in Stage 4 group than in Stage 5 group (p = 0.002). Multivariate regression analysis adjusted for baseline characteristics showed that the cholesterol proportions in very small HDLs were associated with eGFR change rate [F19 β = -17.63, p = 0.036] and ABI [F19 β = 0.047, p = 0.047] in Stage 4 group, and that serum soluble α-Klotho level was associated with the lipoprotein particle numbers in very small HDLs [F19 β = 0.00026, p = 0.012; F20 β = 0.00041, p = 0.036] in Stage 5 group.ConclusionsThis study showed that HDL subclasses are associated with CKD progression, ABI, and Klotho level in CKD-stage-specific manner.

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Section: Methodssupporting

confidence: 74%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Association of High-Density Lipoprotein Subclasses with Chronic Kidney Disease Progression, Atherosclerosis, and Klotho

Kanda

Okazaki

et al. 2016

PLoS ONE

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“…LipoSEARCH ® uses two tandem TSK-like columns packed with polymethacrylate beads and measures simultaneously cholesterol and TG concentrations of the separated lipoproteins. Detection is performed by absorbance at 550 nm after enzymatic reaction of TG and cholesterol [50]. Automated data-processing software then deconvolutes chromatographic peaks and calculates the lipid concentrations associated with each lipoprotein subclass.…”

Section: Gel Permeation (Gp) Chromatographymentioning

confidence: 99%

“…Automated data-processing software then deconvolutes chromatographic peaks and calculates the lipid concentrations associated with each lipoprotein subclass. Corresponding particle numbers can additionally be derived by the dedicated software using an algorithm developed and patented by Okazaki in 2015 that calculates particle number concentrations from TG and cholesterol concentrations and particle size [50,51].…”

Section: Gel Permeation (Gp) Chromatographymentioning

confidence: 99%

Advanced lipoprotein testing for cardiovascular diseases risk assessment: a review of the novel approaches in lipoprotein profiling

Clouet-Foraison

Gaie-Levrel

Gillery

et al. 2017

Clinical Chemistry and Laboratory Medicine (CCLM)

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Abstract:With the increasing prevalence of cardiovascular diseases (CVD) worldwide, finding reliable and clinically relevant biomarkers to predict acute cardiovascular events has been a major aim of the scientific and medical community. Improvements of the understanding of the pathophysiological pathways of the disease highlighted the major role of lipoprotein particles, and these past decades have seen the emergence of a number of new methodologies to separate, measure and quantitate lipoproteins. Those methods, also known as advanced lipoprotein testing methods (ALT), have gained acceptance in the field of CVD risk assessment and have proven their clinical relevance. In the context of worldwide standardization and harmonization of biological assays, efforts have been initiated toward standardization of ALT methods. However, the complexity of lipoprotein particles and the multiple approaches and methodologies reported to quantify them have rendered these initiatives a critical issue. In this context and to better understand these challenges, this review presents a summary of the major methods available for ALT with the aim to point out the major differences in terms of procedures and quantities actually measured and to discuss the resulting comparability issues.

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Oxidized Low-Density Lipoprotein: Preparation, Validation, and Use in Cell Models

Angayarkanni,

Anand Babu

2024

Methods in Molecular Biology

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Recent Advances in Analytical Methods on Lipoprotein Subclasses: Calculation of Particle Numbers from Lipid Levels by Gel Permeation HPLC Using “Spherical Particle Model”

Cited by 65 publications

References 99 publications

Association of High-Density Lipoprotein Subclasses with Chronic Kidney Disease Progression, Atherosclerosis, and Klotho

Association of High-Density Lipoprotein Subclasses with Chronic Kidney Disease Progression, Atherosclerosis, and Klotho

Advanced lipoprotein testing for cardiovascular diseases risk assessment: a review of the novel approaches in lipoprotein profiling

Oxidized Low-Density Lipoprotein: Preparation, Validation, and Use in Cell Models

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