Recent advances and trends in sample preparation and chemical modification for glycan analysis

Kinoshita, Mitsuhiro; Yamada, Keita

doi:10.1016/j.jpba.2021.114424

Cited by 18 publications

(9 citation statements)

References 185 publications

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“…On the other hand, the purification of procainamide labeled N- glycans may also affect the data obtained from N- glycan analysis. In the literature, many purification methods, including different interactions between N- glycans and adsorbents, have been introduced [ 21 , 22 ]. For HILIC-FLD-based N- glycosylation analysis, hydrophilic-interaction-based sorbents have commonly been employed for the purifications [ 23 ].…”

Section: Resultsmentioning

confidence: 99%

Comparison of denaturing agent effects in enzymatic N-glycan release for human plasma N-glycan analysis

KAYILI¹,

SAKHTA²,

SALİH³

2022

Turkish Journal of Chemistry

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Glycosylation is an essential posttranslational modification observed in the living proteome. Glycosylation profiles in glycoproteins can change in commonly observed diseases such as cancer. Identifying these changes is crucial in discovering new biomarkers for the early diagnosis of cancer. One of the main steps of N -glycan analysis is to release N -glycans from glycoproteins by specific enzymes. The study compares common denaturing agent combinations used in N -glycan release methods. In the study, human plasma was used to test the release methods of N -glycans containing different detergent combinations. The released N -glycans were labeled with the procainamide tag, purified using cellulose-containing solid-phase extraction cartridges, and analyzed by high-performance liquid chromatography-hydrophilic interaction liquid chromatography equipped with fluorescence detection (HPLC-HILIC-FLD). The results showed that the sodium dodecyl sulfate and sodium deoxycholate (SDS + SDC) detergent combination provided the highest average FLD signal areas and intensities in the N -glycan analysis. The protocol with SDS resulted in more reproducible average FLD signal areas and intensities. It was also found that the average signal FLD signal areas and intensities of the detected N -glycans were reduced when SDS and SDC were used with 1,4-dithiothreitol (DTT) reducing agents. In addition, deglycosylation of glycoproteins with various denaturing agents resulted in relatively minor variation in human plasma N -glycosylation profiles.

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Section: Resultsmentioning

confidence: 99%

Comparison of denaturing agent effects in enzymatic N-glycan release for human plasma N-glycan analysis

KAYILI¹,

SAKHTA²,

SALİH³

2022

Turkish Journal of Chemistry

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“…These glycosylation enrichment methods facilitate the purification of EVs for subsequent analysis. The advantages and disadvantages of these enrichment techniques are extensively discussed in the fields of glycoproteomics and glycomics and will not be further elaborated upon here. ,− The following section primarily focuses on reviewing the analytical methods for glycosylation.…”

Section: The Research Of Glycosylation In Evsmentioning

confidence: 99%

Comprehensive Overview the Role of Glycosylation of Extracellular Vesicles in Cancers

Wu,

Gao

2023

ACS Omega

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Extracellular vesicles (EVs) are membranous structures secreted by various cells carrying diverse biomolecules. Recent advancements in EV glycosylation research have underscored their crucial role in cancer. This review provides a global overview of EV glycosylation research, covering aspects such as specialized techniques for isolating and characterizing EV glycosylation, advances on how glycosylation affects the biogenesis and uptake of EVs, and the involvement of EV glycosylation in intracellular protein expression, cellular metastasis, intercellular interactions, and potential applications in immunotherapy. Furthermore, through an extensive literature review, we explore recent advances in EV glycosylation research in the context of cancer, with a focus on lung, colorectal, liver, pancreatic, breast, ovarian, prostate, and melanoma cancers. The primary objective of this review is to provide a comprehensive update for researchers, whether they are seasoned experts in the field of EVs or newcomers, aiding them in exploring new avenues and gaining a deeper understanding of EV glycosylation mechanisms. This heightened comprehension not only enhances researchers’ knowledge of the pathogenic mechanisms of EV glycosylation but also paves the way for innovative cancer diagnostic and therapeutic strategies.

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“…Kinoshita and Yamada [ 165 ] in their very recent review summarized the trends in modern sample preparation and chemical modification methods for the structural and quantitative glycan analyses together with their challenges and advantages. Further up-to-date information concerning the glycan labeling can be thus found there.…”

Section: Glycan Separationmentioning

confidence: 99%

Liquid chromatography and capillary electrophoresis in glycomic and glycoproteomic analysis

et al. 2022

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Glycosylation is one of the most significant and abundant post-translational modifications in cells. Glycomic and glycoproteomic analyses involve the characterization of oligosaccharides (glycans) conjugated to proteins. Glycomic and glycoproteomic analysis is highly challenging because of the large diversity of structures, low abundance, site-specific heterogeneity, and poor ionization efficiency of glycans and glycopeptides in mass spectrometry (MS). MS is a key tool for characterization of glycans and glycopeptides. However, MS alone does not always provide full structural and quantitative information for many reasons, and thus MS is combined with some separation technique. This review focuses on the role of separation techniques used in glycomic and glycoproteomic analyses, liquid chromatography and capillary electrophoresis. The most important separation conditions and results are presented and discussed. Graphical abstract

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Recent advances and trends in sample preparation and chemical modification for glycan analysis

Cited by 18 publications

References 185 publications

Comparison of denaturing agent effects in enzymatic N-glycan release for human plasma N-glycan analysis

Comparison of denaturing agent effects in enzymatic N-glycan release for human plasma N-glycan analysis

Comprehensive Overview the Role of Glycosylation of Extracellular Vesicles in Cancers

Liquid chromatography and capillary electrophoresis in glycomic and glycoproteomic analysis

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