Recent Advances and Future Perspectives on Microfluidic Liquid Handling

Nguyen, Nam‐Trung; Hejazian, Majid; Ooi, Chin Hong; Kashaninejad, Navid

doi:10.3390/mi8060186

Cited by 146 publications

(110 citation statements)

References 140 publications

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“…Microfluidics is the science and technology of handling a small volume of fluids in the channels with sub-millimetre length scale [67,68]. As a science and technology, microfluidics can be used for various fluid mechanics applications, including slip flow in superhydrophobic microchannels [69,70] and drag reduction [71][72][73].…”

Section: Microfluidic Methods For Spheroid Culturementioning

confidence: 99%

Inventions and Innovations in Preclinical Platforms for Cancer Research

2018

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Three-dimensional (3D) cell culture systems can be regarded as suitable platforms to bridge the huge gap between animal studies and two-dimensional (2D) monolayer cell culture to study chronic diseases such as cancer. In particular, the preclinical platforms for multicellular spheroid formation and culture can be regarded as ideal in vitro tumour models. The complex tumour microenvironment such as hypoxic region and necrotic core can be recapitulated in 3D spheroid configuration. Cells aggregated in spheroid structures can better illustrate the performance of anti-cancer drugs as well. Various methods have been proposed so far to create such 3D spheroid aggregations. Both conventional techniques and microfluidic methods can be used for generation of multicellular spheroids. In this review paper, we first discuss various spheroid formation phases. Then, the conventional spheroid formation techniques such as bioreactor flasks, liquid overlay and hanging droplet technique are explained. Next, a particular topic of the hydrogel in spheroid formation and culture is explored. This topic has received less attention in the literature. Hydrogels entail some advantages to the spheroid formation and culture such as size uniformity, the formation of porous spheroids or hetero-spheroids as well as chemosensitivity and invasion assays and protecting from shear stress. Finally, microfluidic methods for spheroid formation and culture are briefly reviewed.

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Section: Microfluidic Methods For Spheroid Culturementioning

confidence: 99%

Inventions and Innovations in Preclinical Platforms for Cancer Research

2018

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“…DMF based devices not only overcome the limitations of diffusion based laminar mixing of reagents which is present in continuous microfluidic devices due to low Reynolds number laminar flows; they also offer the ability to have precision control over droplet interactions in a rapid fashion. Several review articles explore the wide field of continuous and discrete microfluidics [47][48][49] • ], as such here we shall limit our discussion to the use of dielectrowetting based DMF devices. A thorough review of dielectrowetting in microfluidics is covered in a recent review by Geng and Cho [50].…”

Section: Dielectrowetting In Microfluidicsmentioning

confidence: 99%

Dielectrowetting: The past, present and future

Edwards

Brown

Newton

et al. 2018

Current Opinion in Colloid & Interface Science

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Liquid dielectrophoresis is a bulk force acting on dipoles within a dielectric liquid inside a non-uniform electric field. When the driving electrodes are interdigitated, bulk liquid dielectrophoresis is converted to an interfacelocalised form capable of modifying the energy balance at an interface. When the interface is a solid-liquid one, the wetting properties of a surface are modified and this approach is known as dielectrowetting. Dielectrowetting has been shown to provide the ability to reversibly modify the contact angle of a liquid droplet with the application of voltage, the strength of which is controlled by the penetration depth of the non-uniform field and permittivities of the fluids involved. Importantly, dielectrowetting provides the ability to create thin liquid films, overcoming the limitation of contact angle saturation present in electrowetting. In this paper, we review the development of dielectrowetting -its origins, the statics and dynamics of dielectrowetted droplets, and the applications of dielectrowetting in microfluidics and optofluidics. Recent developments in the field are also reviewed showing the future directions of this rapidly developing field.

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“…Liquid marbles are a new, but strong, player in the field of digital microfluidics [17]. Microfluidics involves the formation, behavior, and ultimately the control of microliter quantities of fluid.…”

Section: Liquid Marblesmentioning

confidence: 99%