Recent Advances and Current Trends in Transmission Tomographic Diffraction Microscopy

Abstract

Optical microscopy techniques are among the most used methods in biomedical sample characterization. In their more advanced realization, optical microscopes demonstrate resolution down to the nanometric scale. These methods rely on the use of fluorescent sample labeling in order to break the diffraction limit. However, fluorescent molecules’ phototoxicity or photobleaching is not always compatible with the investigated samples. To overcome this limitation, quantitative phase imaging techniques have been proposed. Among these, holographic imaging has demonstrated its ability to image living microscopic samples without staining. However, for a 3D assessment of samples, tomographic acquisitions are needed. Tomographic Diffraction Microscopy (TDM) combines holographic acquisitions with tomographic reconstructions. Relying on a 3D synthetic aperture process, TDM allows for 3D quantitative measurements of the complex refractive index of the investigated sample. Since its initial proposition by Emil Wolf in 1969, the concept of TDM has found a lot of applications and has become one of the hot topics in biomedical imaging. This review focuses on recent achievements in TDM development. Current trends and perspectives of the technique are also discussed.

Label-Free Three-Dimensional Morphological Characterization of Cell Death Using Holographic Tomography

“…Holographic tomography (HT) [ 22 , 23 , 24 , 25 ] is a powerful label-free imaging technique that enables the observation and quantitative analysis of cells and their internal organelles in three dimensions (3D) based on refractive index (RI). The RI distribution of a cell is sensitively related to its mass distribution [ 26 ], and different organelles exhibit distinct RI distributions.…”