Recent advance in cell patterning techniques: Approaches, applications and future prospects

Qin, Yitong; Wang, Zhen; Yu, Tao; Chen, Yibao; Ge, Zhixing

doi:10.1016/j.sna.2021.113229

Cited by 18 publications

(13 citation statements)

References 212 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In cell patterning, the conventional homogeneous surface that cells adhere to and grow is replaced by an engineered geometric surface with variable microtopography, allowing control of the size and shape of cells. [50] Cell patterning techniques are divided into physical methods [51] and biochemical methods. [52] These strategies have been used to study fundamental principles of cell biology (such as differentiation, proliferation, and migration) [53] and in drug screening.…”

Section: D Cell Culture Methodsmentioning

confidence: 99%

Recent Advances on Cell Culture Platforms for In Vitro Drug Screening and Cell Therapies: From Conventional to Microfluidic Strategies

Cardoso

Castanheira

Lanceros‐Méndez

et al. 2023

Adv Healthcare Materials

View full text Add to dashboard Cite

The clinical translations of drugs and nanomedicines depend on coherent pharmaceutical research based on biologically accurate screening approaches. Since establishing the 2D in vitro cell culture method, the scientific community has improved cell-based drug screening assays and models. Those advances result in more informative biochemical assays and the development of 3D multicellular models to describe the biological complexity better and enhance the simulation of the in vivo microenvironment. Despite the overall dominance of conventional 2D and 3D cell macroscopic culture methods, they present physicochemical and operational challenges that impair the scale-up of drug screening by not allowing a high parallelization, multidrug combination, and high-throughput screening. Their combination and complementarity with microfluidic platforms enable the development of microfluidics-based cell culture platforms with unequivocal advantages in drug screening and cell therapies. Thus, this review presents an updated and consolidated view of cell culture miniaturization's physical, chemical, and operational considerations in the pharmaceutical research scenario. It clarifies advances in the field using gradient-based microfluidics, droplet-based microfluidics, printed-based microfluidics, digital-based microfluidics, SlipChip, and paper-based microfluidics. Finally, it presents a comparative analysis of the performance of cell-based methods in life research and development to achieve increased precision in the drug screening process.

show abstract

Section: D Cell Culture Methodsmentioning

confidence: 99%

Recent Advances on Cell Culture Platforms for In Vitro Drug Screening and Cell Therapies: From Conventional to Microfluidic Strategies

Cardoso

Castanheira

Lanceros‐Méndez

et al. 2023

Adv Healthcare Materials

View full text Add to dashboard Cite

show abstract

“…Further, the addition of both the interface-induced adhesion and the eliminating maximization ensured the high probability of producing the best cell patterning, which has never been achieved previously. [7,31,32] The application of TH membranes with different definitions intensified the geometric diversity of cell patterns on the chips. In addition, the patterned array can be conveniently applied for on-chip and massive cell investigation based on optical/fluorescent imaging and quantitative analysis.…”

Section: Principle Of Th Membrane-assisted Cell Patterningmentioning

confidence: 99%

“…This progress was entirely not achieved in the previous patterning researches. [7,27,[35][36][37] The perfect background elimination was attributed to both the membrane-assisted cleaning of most adhesive cells and the two rinsing processes being critical for removing the low-adherent and suspended cells.…”

Section: Cell Patterning With Background Eliminationmentioning

confidence: 99%

“…Thus, many methodological approaches of cell patterning have been achieved by global scholars depending on different trapping and adhesion mechanisms associated with both the external force (e.g., hydrodynamic, acoustic, and dielectrophoretic forces) direction [3][4][5][6] and the selective adhesion on the micro/nano-topographical interfaces or modified substrates, DOI: 10.1002/mabi.202300267 which were implemented by electrohydrodynamic jet printing or laser ablation, microstencilling, microcontact printing, microfluidic printing, and so on. [7][8][9][10] The established tools have more or less exhibited attractive manipulation features like simple/rapid trapping, precise positioning, and throughput operation. The superiority of these tools facilitate diverse living cell-based applications (e.g., cell interaction, single cell array, hepatic lobule, tumor, and neuronal network).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Straightforward Cell Patterning with Ultra‐Low Background Using Polydimethylsiloxane Through‐Hole Membranes

Zhou,

Sun,

Xuanyuan

et al. 2023

Macromolecular Bioscience

View full text Add to dashboard Cite

Micropatterning is becoming an increasingly popular tool to realize microscale cell positioning and decipher cell activities and functions under specific microenvironments. However, a facile methodology for building a highly precise cell pattern still remains challenging. In this study, A simple and straightforward method for stable and efficient cell patterning with ultra‐low background using polydimethylsiloxane through‐hole membranes is developed. The patterning process is conveniently on the basis of membrane peeling and routine pipetting. Cell patterning in high quality involving over 97% patterning coincidence and zero residue on the background is achieved. The high repeatability and stability of the established method for multiple types of cell arrangements with different spatial profiles is demonstrated. The customizable cell patterning with ultra‐low background and high diversity is confirmed to be quite feasible and reliable. Furthermore, the applicability of the patterning method for investigating the fundamental cell activities is also verified experimentally. We believe this microengineering advancement has valuable applications in many microscale cell manipulation‐associated research fields including cell biology, cell engineering, cell imaging, and cell sensing.This article is protected by copyright. All rights reserved

show abstract

“…Nanoscience and nanotechnology are nowadays a fertile groundwork of materials and nanoscopic light-matter phenomena that provide unique solutions in endless scenarios. Within this field, the patterning of biomacromolecules points toward a promising scope in biomedical applications such as organ-on-a-chip, , neuronal networks, − drug delivery, and implant coatings among others. It also involves a particularly high impact in biosensing, where the biomolecular patterns are tailored to display nanoscopic phenomena to transduce biorecognition events. , A crucial aspect in this scenario is the development of fast and large-scale methods to fabricate active nanostructures with a high geometrical accuracy.…”

Section: Introductionmentioning

confidence: 99%

Denaturing for Nanoarchitectonics: Local and Periodic UV-Laser Photodeactivation of Protein Biolayers to Create Functional Patterns for Biosensing

Juste-Dolz

Delgado-Pinar

Avella-Oliver

et al. 2022

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

The nanostructuration of biolayers has become a paradigm for exploiting nanoscopic light-matter phenomena for biosensing, among other biomedical purposes. In this work, we present a photopatterning method to create periodic structures of biomacromolecules, based on a local and periodic mild denaturation of protein biolayers mediated by UV-laser irradiation. These nanostructures are constituted by a periodic modulation of the protein activity, so they are free of topographic and compositional changes along the pattern. Herein we introduce the approach, explore the patterning parameters, characterize the resulting structures, and assess their overall homogeneity. This UV-based patterning principle has proven to be an easy, cost-effective, and fast way to fabricate large areas of homogeneous one-dimensional protein patterns (2 min, 15 x 1.2 mm, relative standard deviation ≃ 16%). This work also investigates the implementation of these protein patterns as transducers for 2 diffractive biosensing. Using a model immunoassay, these patterns have demonstrated negligible signal contributions from non-specific bindings and comparable experimental limits of detection in buffer medium and in human serum (53 and 36 ng•mL -1 of unlabelled IgG, respectively).

show abstract

Recent advance in cell patterning techniques: Approaches, applications and future prospects

Cited by 18 publications

References 212 publications

Recent Advances on Cell Culture Platforms for In Vitro Drug Screening and Cell Therapies: From Conventional to Microfluidic Strategies

Recent Advances on Cell Culture Platforms for In Vitro Drug Screening and Cell Therapies: From Conventional to Microfluidic Strategies

Straightforward Cell Patterning with Ultra‐Low Background Using Polydimethylsiloxane Through‐Hole Membranes

Denaturing for Nanoarchitectonics: Local and Periodic UV-Laser Photodeactivation of Protein Biolayers to Create Functional Patterns for Biosensing

Contact Info

Product

Resources

About