Recellularized rat testis scaffolds with embryoid bodies cells: a promising approach for tissue engineering

Batista, Vitória Frias; Matias, Gustavo de Sá Schiavo; Carreira, Ana Cláudia Oliveira; Smith, Lawrence C.; Rodrigues, Rafaela; Araujo, Michelle Silva; Silva, Dara Rúbia Souza; Moraes, Felipe de Jesus; Garcia, Joaquim Mansano; Miglino, María Angélica

doi:10.1080/19396368.2021.2007554

Cited by 2 publications

(2 citation statements)

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“…The scaffold also provides a basis for performing tissue recellularization by culturing isolated cells in vitro or in the host in vivo by cell migration ( 223 ). Several types of stem cells can be used in the differentiation of germ cells, especially in cases of disturbances in spermatogonia ( 60 ).…”

Section: Discussionmentioning

confidence: 99%

“…The extracellular matrix is a major player in this process, as the communication and molecular signaling of the components of the ECM with the spermatozoon not only provides structural support to the gamete, but also plays an active role in maintaining its viability, as well as in the transport and distribution of essential substances to the gamete (248). Recellularized testicular ECM may be a promissing tool for future new approaches for testicular cell differentiation applied for assisted reproduction techniques and infertility treatments (223) Decellularized scaffolds 100mm slices were decellularized with 1% SDS immersed for 24 hours.…”

Section: Spermatogonial Stem Cellsmentioning

confidence: 99%

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Biomaterials for Testicular Bioengineering: How far have we come and where do we have to go?

et al. 2023

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Traditional therapeutic interventions aim to restore male fertile potential or preserve sperm viability in severe cases, such as semen cryopreservation, testicular tissue, germ cell transplantation and testicular graft. However, these techniques demonstrate several methodological, clinical, and biological limitations, that impact in their results. In this scenario, reproductive medicine has sought biotechnological alternatives applied for infertility treatment, or to improve gamete preservation and thus increase reproductive rates in vitro and in vivo. One of the main approaches employed is the biomimetic testicular tissue reconstruction, which uses tissue-engineering principles and methodologies. This strategy pursues to mimic the testicular microenvironment, simulating physiological conditions. Such approach allows male gametes maintenance in culture or produce viable grafts that can be transplanted and restore reproductive functions. In this context, the application of several biomaterials have been proposed to be used in artificial biological systems. From synthetic polymers to decellularized matrixes, each biomaterial has advantages and disadvantages regarding its application in cell culture and tissue reconstruction. Therefore, the present review aims to list the progress that has been made and the continued challenges facing testicular regenerative medicine and the preservation of male reproductive capacity, based on the development of tissue bioengineering approaches for testicular tissue microenvironment reconstruction.

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Section: Discussionmentioning

confidence: 99%

Section: Spermatogonial Stem Cellsmentioning

confidence: 99%